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AB162807

Recombinant Human UGT1A1 protein (GST tag N-Terminus)

Recombinant Human UGT1A1 protein (GST tag N-Terminus)

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Recombinant Human UGT1A1 protein (GST tag N-Terminus) is a Human Fragment protein, in the 54 to 159 aa range, expressed in Wheat germ, suitable for ELISA, WB.

別名を表示する

GNT1, UGT1, UGT1A1, UDP-glucuronosyltransferase 1A1, Bilirubin-specific UDPGT isozyme 1, UDP-glucuronosyltransferase 1-1, UDP-glucuronosyltransferase 1A isoform 1, hUG-BR1, UDPGT 1-1, UGT1*1, UGT1-01, UGT1.1

1 Images
SDS-PAGE - Recombinant Human UGT1A1 protein (GST tag N-Terminus) (AB162807)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human UGT1A1 protein (GST tag N-Terminus) (AB162807)

ab162807 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

発現系

Wheat germ

タグ

GST tag N-Terminus

アプリケーション

WB, ELISA

applications

生物活性

No

アクセッション番号

P22309

アニマルフリー

No

キャリアフリー

No

種

Human

バッファー組成

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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配列情報

[{"sequence":"GHEIVVLAPDASLYIRDGAFYTLKTYPVPFQREDVKESFVSLGHNVFENDSFLQRVIKTYKKIKKDSAMLLSGCSHLLHNKELMASLAESSFDVMLTDPFLPCSPI","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":159,"aminoAcidStart":54,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P22309","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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出荷温度及び保存条件

出荷温度
Dry Ice
短期保存温度
-80°C
長期保存温度
-80°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
False
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

UGT1A1 also known as UDP-glucuronosyltransferase 1A1 is an enzyme critical for conjugating bilirubin and various hormones with glucuronic acid. It has a molecular mass of approximately 54 kDa. The enzyme primarily localizes in the liver but is also found in other tissues such as the intestine and kidney. UGT1A1's role involves transforming lipophilic molecules into more water-soluble forms facilitating their excretion from the body.
Biological function summary

UGT1A1 contributes to the detoxification processes by mediating the conjugation of bilirubin a byproduct of red blood cell breakdown. This enzyme is part of the UGT1 complex which includes several enzymes sharing a similar expression pattern and function. Through its activity UGT1A1 helps maintain homeostasis and prevents the buildup of potentially toxic substances in the organism.

Pathways

UGT1A1 is a significant component of the glucuronidation pathway. This pathway is essential for phase II metabolism in the liver working alongside other proteins like UGT1A6 and UGT1A9. The glucuronidation pathway transforms hydrophobic compounds into hydrophilic derivatives for easier elimination. Additionally UGT1A1 activity affects hormone metabolism particularly in the pathways involving estrogen and thyroid hormones.

Mutations or polymorphisms in UGT1A1 relate to conditions such as Gilbert’s syndrome and Crigler-Najjar syndrome. These diseases involve impaired bilirubin clearance leading to jaundice. Connection with other proteins like albumin highlights the collaborative role in bilirubin transport and clearance. Studying UGT1A1 and its interactions continues to be critical for understanding and treating these metabolic disorders.
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製品の性状

製品の状態

Liquid

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一般的な情報

機能

Isoform 1. UDP-glucuronosyltransferase (UGT) that catalyzes phase II biotransformation reactions in which lipophilic substrates are conjugated with glucuronic acid to increase the metabolite's water solubility, thereby facilitating excretion into either the urine or bile (PubMed : 12181437, PubMed : 15472229, PubMed : 18004206, PubMed : 18004212, PubMed : 18719240, PubMed : 19830808, PubMed : 23288867, PubMed : 15231852, PubMed : 21422672, PubMed : 38211441). Essential for the elimination and detoxification of drugs, xenobiotics and endogenous compounds (PubMed : 12181437, PubMed : 18004206, PubMed : 18004212). Catalyzes the glucuronidation of endogenous estrogen hormones such as estradiol, estrone and estriol (PubMed : 15472229, PubMed : 18719240, PubMed : 23288867). Involved in the glucuronidation of bilirubin, a degradation product occurring in the normal catabolic pathway that breaks down heme in vertebrates (PubMed : 17187418, PubMed : 18004206, PubMed : 19830808, PubMed : 24525562). Involved in the glucuronidation of arachidonic acid (AA) and AA-derived eicosanoids including 15-HETE, 20-HETE, PGB1 and F2-isoprostane (8-iso-PGF2alpha) (PubMed : 15231852, PubMed : 38211441). Involved in the glucuronidation of the phytochemical ferulic acid at the phenolic or the carboxylic acid group (PubMed : 21422672). Also catalyzes the glucuronidation the isoflavones genistein, daidzein, glycitein, formononetin, biochanin A and prunetin, which are phytoestrogens with anticancer and cardiovascular properties (PubMed : 18052087, PubMed : 19545173). Involved in the glucuronidation of the AGTR1 angiotensin receptor antagonist losartan, a drug which can inhibit the effect of angiotensin II (PubMed : 18674515). Involved in the biotransformation of 7-ethyl-10-hydroxycamptothecin (SN-38), the pharmacologically active metabolite of the anticancer drug irinotecan (PubMed : 12181437, PubMed : 18004212, PubMed : 20610558).. Isoform 2. Lacks UGT glucuronidation activity but acts as a negative regulator of isoform 1.

配列の類似性

Belongs to the UDP-glycosyltransferase family.

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製品プロトコール

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ターゲットの情報

Isoform 1. UDP-glucuronosyltransferase (UGT) that catalyzes phase II biotransformation reactions in which lipophilic substrates are conjugated with glucuronic acid to increase the metabolite's water solubility, thereby facilitating excretion into either the urine or bile (PubMed : 12181437, PubMed : 15472229, PubMed : 18004206, PubMed : 18004212, PubMed : 18719240, PubMed : 19830808, PubMed : 23288867, PubMed : 15231852, PubMed : 21422672, PubMed : 38211441). Essential for the elimination and detoxification of drugs, xenobiotics and endogenous compounds (PubMed : 12181437, PubMed : 18004206, PubMed : 18004212). Catalyzes the glucuronidation of endogenous estrogen hormones such as estradiol, estrone and estriol (PubMed : 15472229, PubMed : 18719240, PubMed : 23288867). Involved in the glucuronidation of bilirubin, a degradation product occurring in the normal catabolic pathway that breaks down heme in vertebrates (PubMed : 17187418, PubMed : 18004206, PubMed : 19830808, PubMed : 24525562). Involved in the glucuronidation of arachidonic acid (AA) and AA-derived eicosanoids including 15-HETE, 20-HETE, PGB1 and F2-isoprostane (8-iso-PGF2alpha) (PubMed : 15231852, PubMed : 38211441). Involved in the glucuronidation of the phytochemical ferulic acid at the phenolic or the carboxylic acid group (PubMed : 21422672). Also catalyzes the glucuronidation the isoflavones genistein, daidzein, glycitein, formononetin, biochanin A and prunetin, which are phytoestrogens with anticancer and cardiovascular properties (PubMed : 18052087, PubMed : 19545173). Involved in the glucuronidation of the AGTR1 angiotensin receptor antagonist losartan, a drug which can inhibit the effect of angiotensin II (PubMed : 18674515). Involved in the biotransformation of 7-ethyl-10-hydroxycamptothecin (SN-38), the pharmacologically active metabolite of the anticancer drug irinotecan (PubMed : 12181437, PubMed : 18004212, PubMed : 20610558).. Isoform 2. Lacks UGT glucuronidation activity but acts as a negative regulator of isoform 1.
See full target information UDP-glucuronosyltransferase 1A1
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組合せ製品

Primary Antibodies

AB170858

Anti-UGT1A1 antibody [EPR9592]

RabMAb|Recombinant

Western blot - Anti-UGT1A1 antibody [EPR9592] (AB170858)

  • 4
  • 1
Assay Kits

AB273331

UGT Activity Assay / Ligand Screening Kit (Fluorometric)

Functional Studies - UGT Activity Assay / Ligand Screening Kit (Fluorometric) (AB273331)

  • 0
  • 0
Proteins & Peptides

AB159886

Recombinant Human ZP2 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human ZP2 protein (GST tag N-Terminus) (AB159886)

  • 0
  • 0
Proteins & Peptides

AB159808

Recombinant Human ZAN protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human ZAN protein (GST tag N-Terminus) (AB159808)

  • 0
  • 0

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