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AB161390

Recombinant Human UBP43/USP18 protein (GST tag N-Terminus)

Recombinant Human UBP43/USP18 protein (GST tag N-Terminus)

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(1 Publication)

Recombinant Human UBP43/USP18 protein (GST tag N-Terminus) is a Human Fragment protein, in the 273 to 372 aa range, expressed in Wheat germ, suitable for ELISA, WB.

別名を表示する

ISG43, USP18, Ubl carboxyl-terminal hydrolase 18, 43 kDa ISG15-specific protease, ISG15-specific-processing protease, Ubl thioesterase 18, hUBP43

1 Images
SDS-PAGE - Recombinant Human UBP43/USP18 protein (GST tag N-Terminus) (AB161390)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human UBP43/USP18 protein (GST tag N-Terminus) (AB161390)

ab161390 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

発現系

Wheat germ

タグ

GST tag N-Terminus

アプリケーション

WB, ELISA

applications

生物活性

No

アクセッション番号

Q9UMW8

アニマルフリー

No

キャリアフリー

No

種

Human

バッファー組成

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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製品の詳細

This product was previously labelled as UBP43.
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配列情報

[{"sequence":"LYFPQSLDFSQILPMKRESCDAEEQSGGQYELFAVIAHVGMADSGHYCVYIRNAVDGKWFCFNDSNICLVSWEDIQCTYGNPNYHWQETAYLLVYMKMEC","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":372,"aminoAcidStart":273,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q9UMW8","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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出荷温度及び保存条件

出荷温度
Dry Ice
短期保存温度
-80°C
長期保存温度
-80°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
False
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

UBP43 also known as USP18 is a deubiquitinating enzyme that is 43 kilodaltons in mass. This protein removes ubiquitin from ubiquitinated proteins which regulates their degradation and stability. UBP43 is expressed widely in the body with significant levels in the immune and nervous systems. Its alternate name USP18 reflects its inclusion in the ubiquitin-specific protease family highlighting its specific function in modulating protein ubiquitination.
Biological function summary

UBP43 regulates type I interferon signaling and affects immune response. As a negative feedback regulator it inhibits the interferon signaling pathway preventing unnecessary immune reactions. UBP43 does not form a part of a larger protein complex but acts independently to maintain cellular homeostasis. Its activity impacts the function of other proteins involved in response to viral infections.

Pathways

UBP43 plays an important role in the JAK-STAT signaling pathway interacting with proteins involved in cytokine signaling. It influences the pathway by inhibiting the action of interferon-stimulated genes therefore modulating the immune response. UBP43's interaction with ISG15 a ubiquitin-like modifier is also a critical aspect that affects other cellular processes like protein modification and response to inflammatory stimuli.

UBP43 is closely linked to autoimmune diseases and viral infections. Altered UBP43 activity can lead to improper immune responses contributing to conditions such as systemic lupus erythematosus. It also plays a role in the body's defense against viral infections where its dysregulation may affect the efficiency of antiviral responses. The interplay between UBP43 and ISG15 is of particular interest as changes in their interaction could provide insights into the development or progression of these disorders.
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製品の性状

製品の状態

Liquid

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一般的な情報

機能

Interferon-induced ISG15-specific protease that plays a crucial role for maintaining a proper balance of ISG15-conjugated proteins in cells (PubMed : 11788588). Regulates protein ISGylation by efficiently cleaving ISG15 conjugates linked via isopeptide bonds. Regulates T-cell activation and T-helper 17 (Th17) cell differentiation by deubiquitinating TAK1, likely to keep TAK1-TAB complexes in steady conditions (PubMed : 23825189). In turn, restricts activation of NF-kappa-B, NFAT, and JNK as well as expression of IL2 in T-cells after TCR activation (PubMed : 23825189). Acts as a molecular adapter with USP20 to promote innate antiviral response through deubiquitinating STING1 (PubMed : 27801882). Involved also in the negative regulation of the inflammatory response triggered by type I interferon (PubMed : 27325888, PubMed : 28165510). Upon recruitment by STAT2 to the type I interferon receptor subunit IFNAR2 interferes with the assembly of the ternary interferon-IFNAR1-IFNAR2 complex and acts as a negative regulator of the type I interferon signaling pathway (PubMed : 28165510).. Isoform 2. Has enzymatic activity similar to isoform 1 and interferes with type I interferon signaling. Major deISGylation enzyme for nuclear proteins (PubMed : 22170061).

配列の類似性

Belongs to the peptidase C19 family.

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製品プロトコール

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ターゲットの情報

Interferon-induced ISG15-specific protease that plays a crucial role for maintaining a proper balance of ISG15-conjugated proteins in cells (PubMed : 11788588). Regulates protein ISGylation by efficiently cleaving ISG15 conjugates linked via isopeptide bonds. Regulates T-cell activation and T-helper 17 (Th17) cell differentiation by deubiquitinating TAK1, likely to keep TAK1-TAB complexes in steady conditions (PubMed : 23825189). In turn, restricts activation of NF-kappa-B, NFAT, and JNK as well as expression of IL2 in T-cells after TCR activation (PubMed : 23825189). Acts as a molecular adapter with USP20 to promote innate antiviral response through deubiquitinating STING1 (PubMed : 27801882). Involved also in the negative regulation of the inflammatory response triggered by type I interferon (PubMed : 27325888, PubMed : 28165510). Upon recruitment by STAT2 to the type I interferon receptor subunit IFNAR2 interferes with the assembly of the ternary interferon-IFNAR1-IFNAR2 complex and acts as a negative regulator of the type I interferon signaling pathway (PubMed : 28165510).. Isoform 2. Has enzymatic activity similar to isoform 1 and interferes with type I interferon signaling. Major deISGylation enzyme for nuclear proteins (PubMed : 22170061).
See full target information USP18
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

BMC cancer 25:528 PubMed40122823

2025

Deubiquitinase USP18 mediates cell migration, apoptosis and ferroptosis in lung adenocarcinoma by depending on POU4F1/PRKAA2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xinping Pan,Hui Deng
View all publications
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組合せ製品

Cell Lines & Lysates

AB267177

Human USP18 (UBP43) knockout A549 cell line

Immunocytochemistry/ Immunofluorescence - Human USP18 (UBP43) knockout A549 cell line (AB267177)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB268020

Anti-Fibronectin antibody [EPR23110-46]

KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR23110-46] (AB268020)

  • 4
  • 5
Primary Antibodies

AB187156

Anti-USP25 antibody [EPR15019]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-USP25 antibody [EPR15019] (AB187156)

  • 0
  • 0

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