Recombinant Human UBE2M/UBC12 protein (ab185866)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Suitable for: HPLC, SDS-PAGE
製品の詳細
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製品名
Recombinant Human UBE2M/UBC12 protein
UBE2M/UBC12 タンパク質・ペプチド 製品一覧 -
精製度
> 95 % SDS-PAGE.
Purity is greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. -
エンドトキシン・レベル
< 1.000 Eu/µg -
発現系
Escherichia coli -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
MIKLFSLKQQKKEEESAGGTKGSSKKASAAQLRIQKDINELNLPKTCDIS FSDPDDLLNFKLVICPDEGFYKSGKFVFSFKVGQGYPHDPPKVKCETMVY HPNIDLEGNVCLNILREDWKPVLTINSIIYGLQYLFLEPNPEDPLNKEAA EVLQNNRRLFEQNVQRSMRGGYIGSTYFERCLK -
予測される分子量
21 kDa -
領域
1 to 183
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab185866 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
HPLC
SDS-PAGE
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製品の状態
Liquid -
備考
This product was previously labelled as UBE2M
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on Dry Ice. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 1.19% HEPES, 0.03% DTT, 0.88% Sodium chloride, 10% Glycerol (glycerin, glycerine)
関連情報
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別名
- hUbc12
- NEDD8 carrier protein
- NEDD8 conjugating enzyme Ubc12
see all -
機能
Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation. -
パスウェイ
Protein modification; protein neddylation. -
配列類似性
Belongs to the ubiquitin-conjugating enzyme family. UBC12 subfamily. -
ドメイン
Both the N-terminal docking peptide and the catalytic core domain must bind the UBA3-NAE1 complex simultaneously for optimal transfer of NEDD8. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab185866 は 1 報の論文で使用されています。
- Shi W et al. E7 Peptide Enables BMSC Adhesion and Promotes Chondrogenic Differentiation of BMSCs Via the LncRNA H19/miR675 Axis. Bioengineering (Basel) 10:N/A (2023). PubMed: 37508808