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AB113402

Recombinant Human UBE2D4 protein (His tag N-Terminus)

Recombinant Human UBE2D4 protein (His tag N-Terminus)

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Recombinant Human UBE2D4 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 147 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

別名を表示する

UBCH5D, UBE2D4, Ubiquitin-conjugating enzyme E2 D4, E2 ubiquitin-conjugating enzyme D4, HBUCE1, Ubiquitin carrier protein D4, Ubiquitin-protein ligase D4

1 Images
SDS-PAGE - Recombinant Human UBE2D4 protein (His tag N-Terminus) (AB113402)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human UBE2D4 protein (His tag N-Terminus) (AB113402)

15% SDS-PAGE of UBE2D4 protein : (1) MW ladder, (2) protein loaded 3ug

Key facts

精製度

>95% SDS-PAGE

Conventional chromatography techniques (anion exchange followed by gel filtration with 20mM Tris pH 7.5, 2mM EDTA).

発現系

Escherichia coli

タグ

His tag N-Terminus

アプリケーション

Mass Spec, SDS-PAGE

applications

生物活性

No

アクセッション番号

Q9Y2X8

アニマルフリー

No

キャリアフリー

No

種

Human

バッファー組成

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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組合せ製品

Proteins & Peptides

AB269094

Recombinant human Rad6 protein (Active)

Functional Studies - Recombinant human Rad6 protein (Active) (AB269094)

  • 0
  • 0
Reagents, Controls & Accessories

AB119211

InstantBlue® Coomassie Protein Stain (ISB1L)

Western blot - InstantBlue® Coomassie Protein Stain (ISB1L) (AB119211)

  • 5
  • 5
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB32072

Anti-c-Myc antibody [Y69] - ChIP Grade

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc antibody [Y69] - ChIP Grade (AB32072)

  • 4
  • 56
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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配列情報

[{"linker":null,"sequence":"MGSSHHHHHHSSGLVPRGSHMALKRIQKELTDLQRDPPAQCSAGPVGDDLFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTKIYHPNINSNGSICLDILRSQWSPALTVSKVLLSICSLLCDPNPDDPLVPEIAHTYKADREKYNRLAREWTQKYAMMALKRIQKELTDLQRDPPAQSAGPVGDDLFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFT","proteinLength":"Full Length","predictedMolecularWeight":"18.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":147,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9Y2X8","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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出荷温度及び保存条件

製品の状態
Liquid
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
False
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

UBE2D4 also known as UBC4/5 homolog is an enzyme with a molecular weight of approximately 16 kDa. This protein functions as a ubiquitin-conjugating enzyme that plays a role in the ubiquitination process helping to transfer ubiquitin from an E1 activating enzyme to an E3 ligase substrate protein. UBE2D4 is expressed in various tissues throughout the body including high expression in skeletal muscle and liver. Its mechanical role is critical in tagging proteins for degradation via the proteasome.
Biological function summary

The ubiquitin-conjugating enzyme plays a role in protein homeostasis by facilitating the ubiquitin-proteasome system a complex that includes E1 E2 and E3 enzymes. UBE2D4 acts in concert with specific E3 ligases to regulate cell cycle progression and transcriptional regulation. This process ensures the removal of misfolded or damaged proteins thereby maintaining protein quality within cells. Its involvement in diverse cellular pathways highlights the enzyme's importance in controlling cellular activities.

Pathways

UBE2D4 operates in the ubiquitin-mediated proteolysis and cell cycle regulatory pathways. These pathways control protein turnover and degradation ensuring cellular equilibrium. UBE2D4 collaborates with E3 ligases like MDM2 within these pathways influencing proteins like p53 which is important for cell cycle control and apoptosis. The interconnectedness with MDM2 emphasizes UBE2D4's role in key cellular events.

Alterations or malfunctions in UBE2D4 activity have linked it to cancer and neurodegenerative diseases. Misregulation of p53 related proteins via UBE2D4 alterations may promote unrestrained cell proliferation seen in cancers. Furthermore abnormal ubiquitination processes associating with pathways including UBE2D4 contribute to the accumulation of protein aggregates in neurodegenerative disorders including Alzheimer's disease. The relationship with MDM2 and p53 proteins highlights UBE2D4's significant role in disease pathogenesis.
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一般的な情報

機能

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11' and 'Lys-48'-linked polyubiquitination.

配列の類似性

Belongs to the ubiquitin-conjugating enzyme family.

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製品プロトコール

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ターゲットの情報

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11' and 'Lys-48'-linked polyubiquitination.
See full target information UBE2D4
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