AB269091

Recombinant human UBE1 protein (Active)

Name: Recombinant human UBE1 protein (Active) (ab269091) | Abcam
Brand: Abcam Limited
SKU: AB269091
Price: 49000 JPY
Availability: InStock

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Recombinant human UBE1 protein (Active) is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with >95%, suitable for SDS-PAGE, FuncS.

別名を表示する

A1S9T, UBE1, UBA1, Ubiquitin-like modifier-activating enzyme 1, Protein A1S9, Ubiquitin-activating enzyme E1

2 Images

Functional Studies - Recombinant human UBE1 protein (Active) (AB269091)

FuncS

Supplier Data

Functional Studies - Recombinant human UBE1 protein (Active) (AB269091)

The specific activity of ab269091 was 110 nmol/min/mg in a ubiquitinating assay using wild-type ubiquitin protein as substrate.

SDS-PAGE - Recombinant human UBE1 protein (Active) (AB269091)

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human UBE1 protein (Active) (AB269091)

SDS-PAGE analysis of ab269091.

Key facts

精製度

>95% SDS-PAGE

発現系

Baculovirus infected Sf9 cells

アプリケーション

SDS-PAGE, FuncS

applications

生物活性

Yes

生物学的活性

The specific activity of ab269091 was 110 nmol/min/mg in a ubiquitinating assay using wild-type ubiquitin protein as substrate.

アクセッション番号

P22314

アニマルフリー

キャリアフリー

種

Human

バッファー組成

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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配列情報

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P22314","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]

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出荷温度及び保存条件

出荷温度

Dry Ice

短期保存温度

-80°C

長期保存温度

-80°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

True

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製品の性状

製品の状態

Liquid

補足情報

Affinity purified.

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一般的な情報

機能

Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system (PubMed : 1447181, PubMed : 1606621, PubMed : 33108101). Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP (PubMed : 1447181). Essential for the formation of radiation-induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites (PubMed : 22456334).

配列の類似性

Belongs to the ubiquitin-activating E1 family.

翻訳後修飾

ISGylated.

細胞内局在性

Mitochondrion

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information UBA1

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Abcam product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Recombinant human UBE1 protein (Active)