AB177256

Recombinant Human UBC6e protein (Tagged)

Name: Recombinant Human UBC6e protein (Tagged) (ab177256) | Abcam
Brand: Abcam Limited
SKU: AB177256
Price: 65000 JPY
Availability: InStock

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Recombinant Human UBC6e protein (Tagged) is a Human Full Length protein, in the 1 to 318 aa range, expressed in Baculovirus infected Sf9 cells, with >70%, suitable for SDS-PAGE, WB.

別名を表示する

NCUBE1, CGI-76, HSPC153, HSPC205, UBE2J1, Ubiquitin-conjugating enzyme E2 J1, E2 ubiquitin-conjugating enzyme J1, Non-canonical ubiquitin-conjugating enzyme 1, Yeast ubiquitin-conjugating enzyme UBC6 homolog E, NCUBE-1, HsUBC6e

1 Images

SDS-PAGE - Recombinant Human UBC6e protein (Tagged) (AB177256)

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human UBC6e protein (Tagged) (AB177256)

SDS-PAGE analysis of ab177256.

Key facts

精製度

>70% Densitometry

発現系

Baculovirus infected Sf9 cells

アプリケーション

SDS-PAGE, WB

applications

生物活性

アクセッション番号

Q9Y385

アニマルフリー

キャリアフリー

種

Human

バッファー組成

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.79% Tris HCl, 0.31% Glutathione, 0.29% Sodium chloride, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

It is recommended to store the protein at -80°C in glycerol to preserve the activity the protein.

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配列情報

[{"sequence":"METRYNLKSPAVKRLMKEAAELKDPTDHYHAQPLEDNLFEWHFTVRGPPDSDFDGGVYHGRIVLPPEYPMKPPSIILLTANGRFEVGKKICLSISGHHPETWQPSWSIRTALLAIIGFMPTKGEGAIGSLDYTPEERRALAKKSQDFCCEGCGSAMKDVLLPLKSGSDSSQADQEAKELARQISFKAEVNSSGKTISESDLNHSFSLTDLQDDIPTTFQGATASTSYGLQNSSAASFHQPTQPVAKNTSMSPRQRRAQQQSQRRLSTSPDVIQGHQPRDNHTDHGGSAVLIVILTLALAALIFRRIYLANEYIFDFEL","proteinLength":"Full Length","predictedMolecularWeight":"70 kDa","actualMolecularWeight":null,"aminoAcidEnd":318,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q9Y385","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]

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出荷温度及び保存条件

出荷温度

Dry Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-80°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

False

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

UBC6e also known as UBE2J2 is a ubiquitin-conjugating enzyme with a molecular mass of approximately 33 kDa. It resides within the membrane of the endoplasmic reticulum (ER) where it functions as an E2 enzyme. UBC6e facilitates the transfer of ubiquitin from E1 activating enzymes to substrate proteins signaling these proteins for degradation. This enzyme's role is essential for controlling protein quality preventing accumulation of misfolded proteins within the ER.

Biological function summary

UBC6e contributes significantly to the ER-associated degradation (ERAD) pathway. The protein operates as part of a larger complex that identifies and targets defective proteins for ubiquitination and subsequent breakdown. Its activity maintains cellular homeostasis by regulating the protein landscape in the ER. The presence of UBC6e ensures only properly folded proteins proceed to the Golgi apparatus reducing the potential for cellular malfunction due to protein misfolding.

Pathways

UBC6e plays a critical role in the unfolded protein response (UPR) and proteostasis networks. The UPR pathway involves proteins like IRE1 and ATF6 working with UBC6e to alleviate ER stress by degrading misfolded proteins. Another pathway related to UBC6e is the proteasome degradation pathway which relies on activities like those of UBC6e and other E2 enzymes to clear misfolded proteins. Together these pathways help the cell manage stress and adapt to environmental changes.

UBC6e has connections with neurodegenerative diseases and certain types of cancer. Its involvement in ERAD relates it to diseases like Alzheimer's where protein misfolding and aggregation are problematic. Furthermore alterations in UBC6e activity intersect with proteins like p53 linking it to cancer progression. Dysregulation of ubiquitination involving UBC6e may contribute to both disease states highlighting its potential as a target for therapeutic intervention.

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製品の性状

製品の状態

Liquid

補足情報

Affinity purified.

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一般的な情報

機能

Catalyzes the covalent attachment of ubiquitin to other proteins. Functions in the selective degradation of misfolded membrane proteins from the endoplasmic reticulum (ERAD) and is essential for cells to recover from ER stress (PubMed : 28321712). Plays a role in MAPKAPK2-dependent translational control of TNF synthesis (PubMed : 24020373). Also acts as a platform for perinuclear positioning of the endosomal system by mediating ubiquitination of SQSTM1 through interaction with the E3 ubiquitin-protein ligase RNF26 (PubMed : 33472082). Plays a role in male fecundity through the interaction with the E3 ubiquitin-protein ligase RNF133 (PubMed : 35831855).. (Microbial infection) Promotes Dengue virus RNA replication by negatively regulating IFN-beta signaling and mediating 'Lys-48'-linked ubiquitination on IRF3 (PubMed : 30157886).

配列の類似性

Belongs to the ubiquitin-conjugating enzyme family.

翻訳後修飾

Phosphorylated at Ser-184 in a cytosolic stress-dependent manner by MAP kinase p38 MAPKAPK2.. Phosphorylated UBE2J1 is rapidly ubiquitinated and subsequently degraded by the proteasome.

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information UBE2J1

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Abcam product promise

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Recombinant Human UBC6e protein (Tagged)