JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB167962

Recombinant Human UBC3B protein

Recombinant Human UBC3B protein

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human UBC3B protein is a Human Full Length protein, in the 2 to 238 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, WB.

別名を表示する

CDC34B, UBC3B, UBE2R2, Ubiquitin-conjugating enzyme E2 R2, E2 ubiquitin-conjugating enzyme R2, Ubiquitin carrier protein R2, Ubiquitin-conjugating enzyme E2-CDC34B, Ubiquitin-protein ligase R2

1 Images
SDS-PAGE - Recombinant Human UBC3B protein (AB167962)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human UBC3B protein (AB167962)

SDS Page analysis of ab167962

Key facts

精製度

>90% SDS-PAGE

発現系

Escherichia coli

タグ

His tag N-Terminus

アプリケーション

WB, SDS-PAGE

applications

生物活性

No

アクセッション番号

Q712K3

アニマルフリー

No

キャリアフリー

No

種

Human

バッファー組成

pH: 7 Preservative: 1.02% Imidazole Constituents: 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.82% Sodium phosphate, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

配列情報

[{"sequence":"AQQQMTSSQKALMLELKSLQEEPVEGFRITLVDESDLYNWEVAIFGPPNTLYEGGYFKAHIKFPIDYPYSPPTFRFLTKMWHPNIYENGDVCISILHPPVDDPQSGELPSERWNPTQNVRTILLSVISLLNEPNTFSPANVDASVMFRKWRDSKGKDKEYAEIIRKQVSATKAEAEKDGVKVPTTLAEYCIKTKVPSNDNSSDLLYDDLYDDDIDDEDEEEEDADCYDDDDSGNEES","proteinLength":"Full Length","predictedMolecularWeight":"32 kDa","actualMolecularWeight":null,"aminoAcidEnd":238,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q712K3","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

出荷温度及び保存条件

出荷温度
Dry Ice
短期保存温度
-80°C
長期保存温度
-80°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
False
style
left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

UBC3B also known as UBE2E2 is a ubiquitin-conjugating enzyme with a molecular mass of approximately 25 kDa. This enzyme plays a pivotal role mechanically in the ubiquitination process where it facilitates the transfer of ubiquitin from a ubiquitin-activating enzyme to substrate proteins. UBC3B is expressed in various tissues including the brain and the reproductive system indicating its broad functional importance in different biological contexts.
Biological function summary

The enzyme takes part in protein degradation processes and regulates the stability of numerous proteins by tagging them for degradation by the proteasome. UBC3B functions as part of multi-protein ubiquitin pathways and interacts with E3 ubiquitin ligases to identify specific proteins for tagging. Its activity helps maintain cellular protein homeostasis and impacts processes like cell cycle regulation and DNA repair.

Pathways

UBC3B is involved in ubiquitin-dependent proteolysis and is critical in the cellular stress response pathways. It collaborates closely with proteins like the E3 ligase HECTD1 within these pathways. This relationship helps regulate protein turnover and the cell's response to stress ensuring damaged or misfolded proteins are efficiently degraded to prevent cellular dysfunction.

Mutations or dysregulation of UBC3B are associated with cancer and neurodegenerative diseases. In cancer the altered ubiquitin pathways can lead to uncontrolled cell proliferation due to disrupted protein degradation. UBC3B interacts with proteins like p53 a tumor suppressor linking it to oncogenic processes. In neurodegenerative diseases the accumulation of ubiquitinated proteins due to erroneous UBC3B activity can cause neuronal cell damage.
style
left-column

製品の性状

製品の状態

Liquid

補足情報

Assessed by densitometry.

style
left-column

一般的な情報

機能

E2 ubiquitin-conjugating enzyme that accepts ubiquitin from an E1 ubiquitin-activating protein, and catalyzes its covalent attachment to other proteins by an E3 ubiquitin-protein ligase complex (PubMed : 12037680, PubMed : 20061386, PubMed : 38326650). In vitro catalyzes monoubiquitination and 'Lys-48'-linked polyubiquitination (PubMed : 12037680, PubMed : 20061386, PubMed : 38326650). Works in collaboration with various Cul1-RING and Cul2-RING E3 ligase complexes (PubMed : 38326650). May be involved in degradation of katenin (PubMed : 12037680).

配列の類似性

Belongs to the ubiquitin-conjugating enzyme family.

style
left-column

製品プロトコール

style
left-column

ターゲットの情報

E2 ubiquitin-conjugating enzyme that accepts ubiquitin from an E1 ubiquitin-activating protein, and catalyzes its covalent attachment to other proteins by an E3 ubiquitin-protein ligase complex (PubMed : 12037680, PubMed : 20061386, PubMed : 38326650). In vitro catalyzes monoubiquitination and 'Lys-48'-linked polyubiquitination (PubMed : 12037680, PubMed : 20061386, PubMed : 38326650). Works in collaboration with various Cul1-RING and Cul2-RING E3 ligase complexes (PubMed : 38326650). May be involved in degradation of katenin (PubMed : 12037680).
See full target information UBE2R2
style
left-column
style
left-column
style
right-column

組合せ製品

Proteins & Peptides

AB269138

Recombinant human Wwp2 protein (Active)

Functional Studies - Recombinant human Wwp2 protein (Active) (AB269138)

  • 0
  • 0
Proteins & Peptides

AB173055

Recombinant Human UBE2Z protein

  • 0
  • 0
Proteins & Peptides

AB140732

Recombinant Human YOD1 protein

SDS-PAGE - Recombinant Human YOD1 protein (AB140732)

  • 0
  • 0
Proteins & Peptides

AB269102

Recombinant Human Ube2H protein (His tag)

SDS-PAGE - Recombinant Human Ube2H protein (His tag) (AB269102)

  • 0
  • 0

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com