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Recombinant Human Uba6 protein (ab163029)

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SDS-PAGE - Recombinant Human Uba6 protein (ab163029)

    Key features and details

    • Expression system: Wheat germ
    • Purity: >= 80% Purified via GST Tag
    • Tags: GST tag N-Terminus
    • Suitable for: WB, ELISA

    製品の詳細

    • 製品名

      Recombinant Human Uba6 protein
      Uba6 タンパク質・ペプチド 製品一覧

    • 精製度

      >= 80 % Purified via GST Tag.
      Glutathione Sepharose

    • 発現系

      Wheat germ

    • アクセッション番号

      A0AVT1-3

    • タンパク質長

      Full length protein

    • Animal free

      No

    • 由来

      Recombinant

      • 生物種

        Human

      • 配列

        MEGSEPVAAHQGEEASCSSWGTGSTNKNLPIMSTASVEIDDALYSRQRYV LGDTAMQKMAKSHVFLSGMGGLGLEIAKNLVLAGIKAVTIHDTEKCQAWD LGTNFFLSEDDVVNKRNRAEAVLKHIAELNPYVHVTSSSVPFNETTDLSF LDKYQCVVLTEMKLPLQKKINDFCRSQCPPIKFISADVHGIWSRLFCDFG DEFEVLDTTGEEPKEIFISNITQANPGIVTCLENHPHKLETGQFLTFREI NGMTGLNGSIQQITVISPFSFSIGDTTELEPYLHGGIAVQVKTPKTVFFE SLERQLKHPKCLIVDFSNPEAPLEIHTAMLALDQFQEKYSRKPNVGCQQD SEELLKLATSISETLEEKVTIEIYGCPNICLLIHKCSVY

      • 予測される分子量

        68 kDa

      • 領域

        1 to 389

      • タグ

        GST tag N-Terminus

      • 配列の追加情報

        This corresponds to isoform 3 which is shorter than isoform 1 and also has some differences in sequence.

    特性

    Our Abpromise guarantee covers the use of ab163029 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • アプリケーション

      Western blot

      ELISA

    • 製品の状態

      Liquid

    • 備考

       

       

    • Concentration information loading...

    前処理および保存

    • 保存方法および安定性

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.31% Glutathione, 0.79% Tris HCl

    関連情報

    • 別名

      • E1-L2
      • FLJ10808
      • FLJ23367
      • Monocyte protein 4
      • MOP 4
      • MOP-4
      • MOP4
      • UBA 6
      • Uba6
      • UBA6 ubiquitin activating enzyme E1
      • UBA6_HUMAN
      • UBE1L2
      • Ubiquitin activating enzyme 6
      • Ubiquitin activating enzyme E1 like 2
      • Ubiquitin activating enzyme E1 like protein 2
      • Ubiquitin like modifier activating enzyme 6
      • Ubiquitin-activating enzyme 6
      • Ubiquitin-activating enzyme E1-like protein 2
      • Ubiquitin-like modifier-activating enzyme 6
      see all

    • 機能

      Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP. Specific for ubiquitin, does not activate ubiquitin-like peptides. Differs from UBE1 in its specificity for substrate E2 charging. Does not charge cell cycle E2s, such as CDC34. Essential for embryonic development. Required for UBD/FAT10 conjugation. Isoform 2 may play a key role in ubiquitin system and may influence spermatogenesis and male fertility.

    • 組織特異性

      Widely expressed. Isoform 2 is predominantly expressed in testis with higher expression in adult testis than in fetal testis.

    • パスウェイ

      Protein modification; protein ubiquitination.

    • 配列類似性

      Belongs to the ubiquitin-activating E1 family.
    • Information by UniProt

    画像

    • SDS-PAGE - Recombinant Human Uba6 protein (ab163029)
      SDS-PAGE - Recombinant Human Uba6 protein (ab163029)
      ab163029 on a 12.5% SDS-PAGE stained with Coomassie Blue.

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab163029 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab163029 は論文での使用が確認できていません。

    レビューと Q&A

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