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Recombinant Human Twinkle protein (ab153425)

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SDS-PAGE - Recombinant Human Twinkle protein (ab153425)

    Key features and details

    • Expression system: Wheat germ
    • Tags: GST tag N-Terminus
    • Suitable for: WB, ELISA

    製品の詳細

    • 製品名

      Recombinant Human Twinkle protein

    • 発現系

      Wheat germ

    • タンパク質長

      Full length protein

    • Animal free

      No

    • 由来

      Recombinant

      • 生物種

        Human

      • 配列

        MFGLTKVTDDTLKRFSVRYLRPARSLVFPWFSPGGSGLRGLKLLEAKCQG DGVSYEETTIPRPSAYHNLFGLPLISRRDAEVVLTSRELDSLALNQSTGL PTLTLPRGTTCLPPALLPYLEQFRRIVFWLGDDLRSWEAAKLFARKLNPK RCFLVRPGDQQPRPLEALNGGFNLSRILRTALPAWHKSIVSFRQLREEVL GELSNVEQAAGLRWSRFPDLNRILKGHRKGELTVFTGPTGSGKTTFISEY ALDLCSQGVNTLWGSFEISNVRLARVMLTQFAEGRLEDQLDKYDHWADRF EDLPLYFMTFHGQQSIRTVIDTMQHAVYVYDICHVIIDNLQFMMGHEQLS TDRIAAQDYIIGVFRKFATDNNCHVTLVIHPRKEDDDKELQTASIFGSAK ASQEADNVLILQDRKLVTGPGKRYLQVSKNRFDGDVGVFPLEFNKNSLTF SIPPKNKARLKKIKDDTGPVAKKPSSGKKGATTQNSEICSGQAPTPDQPD TSKRSK

      • 領域

        1 to 506

      • タグ

        GST tag N-Terminus

    特性

    Our Abpromise guarantee covers the use of ab153425 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • アプリケーション

      Western blot

      ELISA

    • 製品の状態

      Liquid
    • Concentration information loading...

    前処理および保存

    • 保存方法および安定性

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.31% Glutathione, 0.79% Tris HCl

    関連情報

    • 別名

      • Ataxin 8
      • Ataxin8
      • ATXN 8
      • ATXN8
      • C10 orf2
      • C10orf 2
      • C10orf2
      • Chromosome 10 open reading frame 2
      • IOSCA
      • mitochondrial
      • MTDPS7
      • PEO
      • PEO 1
      • PEO1
      • PEO1_HUMAN
      • PEOA3
      • Progressive external ophthalmoplegia 1 protein
      • SANDO
      • SCA 8
      • SCA8
      • T7 gp4 like protein with intramitochondrial nucleoid localization
      • T7 gp4-like protein with intramitochondrial nucleoid localization
      • T7 helicase-related protein with intramitochondrial nucleoid localization
      • T7 like mitochondrial DNA helicase
      • T7-like mitochondrial DNA helicase
      • Twinkle protein
      • Twinkle protein, mitochondrial
      • TWINL
      see all

    • 機能

      Involved in mitochondrial DNA (mtDNA) metabolism. Could function as an adenine nucleotide-dependent DNA helicase. Function inferred to be critical for lifetime maintenance of mtDNA integrity. In vitro, forms in combination with POLG, a processive replication machinery, which can use double-stranded DNA (dsDNA) as template to synthesize single-stranded DNA (ssDNA) molecules. May be a key regulator of mtDNA copy number in mammals.

    • 組織特異性

      High relative levels in skeletal muscle, testis and pancreas. Lower levels of expression in the heart, brain, placenta, lung, liver, kidney, spleen, thymus, prostate, ovary, small intestine, colon and leukocytes. Expression is coregulated with MRPL43.

    • 関連疾患

      Progressive external ophthalmoplegia with mitochondrial DNA deletions, autosomal dominant, 3
      Sensory ataxic neuropathy dysarthria and ophthalmoparesis
      Mitochondrial DNA depletion syndrome 7

    • 配列類似性

      Contains 1 SF4 helicase domain.

    • 細胞内局在

      Mitochondrion matrix > mitochondrion nucleoid. Colocalizes with mtDNA in mitochondrial nucleoids, a nucleoproteins complex consisting of a number of copies of proteins associated with mtDNA, probably involved in mtDNA maintenance and expression.
    • Information by UniProt

    画像

    • SDS-PAGE - Recombinant Human Twinkle protein (ab153425)
      SDS-PAGE - Recombinant Human Twinkle protein (ab153425)
      ab153425 on a 12.5% SDS-PAGE stained with Coomassie Blue.

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab153425 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab153425 は論文での使用が確認できていません。

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