Recombinant human Transferrin protein (ab83560)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant human Transferrin protein
Transferrin タンパク質・ペプチド 製品一覧 -
生理活性
The activity of ab83560 was measured by its ability to support the growth of HepG2 cells under conditions of reduced serum. Typically 0.1 to 1 µg enhances cell proliferation.
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精製度
> 95 % SDS-PAGE. -
発現系
HEK 293 cells -
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
Theoretical sequence: VPDKTVRWCAVSEHEATKCQSFRDHMKSVIPSDGPSVACVKKASY L DCIRAIAANEADAVTLDAGLVYDAYLAPNNLKPVVAEFYGSKED PQTF YYAVAVVKKDSGFQMNQLRGKKSCHTGLGRSAGWNIPIGLL YCDLPEP RKPLEKAVANFFSGSCAPCADGTDFPQLCQLCPGCGCS TLNQYFGYSG AFKCLKNGAGDVAFVKHSTIFENLANKADRDQYEL LCLDNTRKPVDEY KDCHLAQVPSHTVVARSMGGKEDLIWELLNQA QEHFGKDKSKEFQLFS SPHGKDLLFKDSAHGFLKVPPRMDAKMYL GYEYVTAIRNLREGTCQEA PTDECKPVKWCALSHHERLKCDEWSV NSVGKIECVSAETTEDCIAKIM NGEADAMSLDGGFVYIAGKCGLV PVLAENYNKSDNCEDTPEAGYFAVA VVKKSASDLTWDNLKGKKSC HTAVGRTAGWNIPMGLLYNKINHCRFDE FFSEGCAPGSKKDSSLC KLCMGSGLNLCEPNNKEGYYGYTGAFRCLVE KGDVAFVKHQTVPQ NTGGKNPDPWAKNLNEKDYELLCLDGTRKPVEEY ANCHLARAPNH AVVTRKDKEACVHKILRQQQHLFGSNVTDCSGNFCLF RSETKDLL FRDDTVCLAKLHDRNTYEKYLGEEYVKAVGNLRKCSTSSL LEACT FRRP
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab83560 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
Constituents: 10% Trehalose, 1% Human serum albumin
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
関連情報
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別名
- Apotransferrin
- Beta 1 metal binding globulin
- Beta-1 metal-binding globulin
see all -
機能
Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. -
組織特異性
Expressed by the liver and secreted in plasma. -
関連疾患
Defects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia. -
配列類似性
Belongs to the transferrin family.
Contains 2 transferrin-like domains. -
細胞内局在
Secreted. - Information by UniProt
画像
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Sandwich ELISA - Recombinant human Transferrin protein (ab83560)
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SDS-PAGE - Recombinant human Transferrin protein (ab83560)Lane 1 – MW markers; Lane 2 – ab83560; Lane 3 – ab83560 treated with PNGase F to remove potential N-linked glycans; Lane 4 – ab83560 treated with a glycosidase cocktail to remove potential N- and Olinked glycans. Approximately 5 μg of protein was loaded per lane; Gel was stained using Coomassie.
Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. A further drop in MW after treatment with the glycosidase cocktail indicates the presence of O-linked glycans. Additional bands in lane 3 and lane 4 are glycosidase enzymes. -
SDS-PAGE - Recombinant human Transferrin protein (ab83560)A sample of ab83560 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris HCl 2D gel. Approximately 40 μg of protein was load; Gel was stained using Deep Purple™. Spot train indicates presence of multiple isoforms of Transferrin. Spots within the spot train were cut from the gel and identified as Transferrin by protein mass fingerprinting. -
Functional Studies - Recombinant human Transferrin protein (ab83560)Post-translational modifications result in protein heterogeneity. The densitometry scan demonstrates that ab83560 exists in multiple isoforms, which differ according to their level of posttranslational modification. Expression of these isoforms is highly significant for cell biology, as they more closely resemble the native human proteins. The triangle indicates theoretical pI and MW of the protein.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab83560 は論文での使用が確認できていません。