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AB83560

Recombinant human Transferrin protein

Recombinant human Transferrin protein

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Recombinant human Transferrin protein is a Human Full Length protein, expressed in HEK 293 cells, with >95%, suitable for SDS-PAGE, FuncS.

別名を表示する

PRO1400, TF, Serotransferrin, Transferrin, Beta-1 metal-binding globulin, Siderophilin

4 Images
Functional Studies - Recombinant human Transferrin protein (AB83560)
  • FuncS

Unknown

Functional Studies - Recombinant human Transferrin protein (AB83560)

Post-translational modifications result in protein heterogeneity. The densitometry scan demonstrates that ab83560 exists in multiple isoforms, which differ according to their level of posttranslational modification. Expression of these isoforms is highly significant for cell biology, as they more closely resemble the native human proteins. The triangle indicates theoretical pI and MW of the protein.

Sandwich ELISA - Recombinant human Transferrin protein (AB83560)
  • sELISA

Unknown

Sandwich ELISA - Recombinant human Transferrin protein (AB83560)

Standard curve for Transferrin (Analyte : ab83560); dilution range 1pg/ml to 1μg/ml using Capture Antibody Mouse monoclonal [HTF-14] to Transferrin (ab769) at 1μg/ml and Detector Antibody Rabbit polyclonal to Transferrin (ab9538) at 0.5μg/ml.

SDS-PAGE - Recombinant human Transferrin protein (AB83560)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human Transferrin protein (AB83560)

A sample of ab83560 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris HCl 2D gel. Approximately 40 μg of protein was load; Gel was stained using Deep Purple™. Spot train indicates presence of multiple isoforms of Transferrin. Spots within the spot train were cut from the gel and identified as Transferrin by protein mass fingerprinting.

SDS-PAGE - Recombinant human Transferrin protein (AB83560)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human Transferrin protein (AB83560)

Lane 1 – MW markers; Lane 2 – ab83560; Lane 3 – ab83560 treated with PNGase F to remove potential N-linked glycans; Lane 4 – ab83560 treated with a glycosidase cocktail to remove potential N- and Olinked glycans. Approximately 5 μg of protein was loaded per lane; Gel was stained using Coomassie.

Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. A further drop in MW after treatment with the glycosidase cocktail indicates the presence of O-linked glycans. Additional bands in lane 3 and lane 4 are glycosidase enzymes.

Key facts

精製度

>95% SDS-PAGE

発現系

HEK 293 cells

タグ

Tag free

アプリケーション

FuncS, SDS-PAGE

applications

生物活性

Yes

生物学的活性

The activity of ab83560 was measured by its ability to support the growth of HepG2 cells under conditions of reduced serum. Typically 0.1 to 1 μg enhances cell proliferation.

アクセッション番号

P02787

アニマルフリー

No

キャリアフリー

No

種

Human

再構成

Reconstitute in 0.5 mL of PBS

バッファー組成

Constituents: 10% Trehalose, 1% Human serum albumin

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Under reducing conditions this protein migrates as a broad band between 70-80 kDa in SDS-PAGE due to post-translational modifications, in particular glycosylation. This compares with unmodified form that has a predicted MW of 75.2 kDa. ab83560 consists of 5-20% carbohydrate by weight. This protein separates into a number of isoforms with a pI between 6.0-8.0 in 2D PAGE due to modifications, in particular glycosylation. This compares with the unmodified form that has a predicted pI of 6.8.</p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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配列情報

[{"sequence":"Theoretical sequence: \r\nVPDKTVRWCAVSEHEATKCQSFRDHMKSVIPSDGPSVACVKKASY\r\nLDCIRAIAANEADAVTLDAGLVYDAYLAPNNLKPVVAEFYGSKED\r\nPQTFYYAVAVVKKDSGFQMNQLRGKKSCHTGLGRSAGWNIPIGLL\r\nYCDLPEPRKPLEKAVANFFSGSCAPCADGTDFPQLCQLCPGCGCS\r\nTLNQYFGYSGAFKCLKNGAGDVAFVKHSTIFENLANKADRDQYEL\r\nLCLDNTRKPVDEYKDCHLAQVPSHTVVARSMGGKEDLIWELLNQA\r\nQEHFGKDKSKEFQLFSSPHGKDLLFKDSAHGFLKVPPRMDAKMYL\r\nGYEYVTAIRNLREGTCQEAPTDECKPVKWCALSHHERLKCDEWSV\r\nNSVGKIECVSAETTEDCIAKIMNGEADAMSLDGGFVYIAGKCGLV\r\nPVLAENYNKSDNCEDTPEAGYFAVAVVKKSASDLTWDNLKGKKSC\r\nHTAVGRTAGWNIPMGLLYNKINHCRFDEFFSEGCAPGSKKDSSLC\r\nKLCMGSGLNLCEPNNKEGYYGYTGAFRCLVEKGDVAFVKHQTVPQ\r\nNTGGKNPDPWAKNLNEKDYELLCLDGTRKPVEEYANCHLARAPNH\r\nAVVTRKDKEACVHKILRQQQHLFGSNVTDCSGNFCLFRSETKDLL\r\nFRDDTVCLAKLHDRNTYEKYLGEEYVKAVGNLRKCSTSSLLEACT\r\nFRRP","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P02787","tags":[]}]
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出荷温度及び保存条件

出荷温度
Blue Ice
短期保存温度
-20°C
長期保存温度
-20°C
保管に関する情報
Avoid freeze / thaw cycle|Reconstitute for long term storage
True
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Transferrin also known as serotransferrin or siderophilin is a glycoprotein with a mass of approximately 80 kDa. It is primarily synthesized in the liver and subsequently secreted into the bloodstream. Transferrin has an important role in iron transport and maintains iron homeostasis by binding and delivering iron to various tissues throughout the body. It can bind two ferric ions (Fe3+) in association with an anion usually bicarbonate. In biological fluids transferrin exists in serum plasma and other extracellular fluids.
Biological function summary

Transferrin facilitates the transportation of iron ions. It delivers iron to cells by binding to transferrin receptors on cell surfaces forming a complex that gets internalized via receptor-mediated endocytosis. Inside the endosomes acidic conditions cause iron to release from transferrin enabling its utilization in cellular processes like DNA synthesis and electron transport. Transferrin itself acts independently and does not form part of a larger protein complex. Variants of transferrin include mouse transferrin bovine transferrin and biotinylated transferrin each with similar function across different species.

Pathways

Transferrin operates centrally in iron metabolism and homeostasis pathways. It functions in coordination with the transferrin receptor 1 (TfR1) which facilitates cellular uptake of the transferrin-iron complex. Additionally transferrin plays a role in the hepcidin regulatory pathway. Hepcidin modulates iron homeostasis by decreasing iron absorption in the intestine and controlling iron release from macrophages and hepatocytes. Transferrin's ability to bind iron connects it to other iron-containing proteins such as ferritin which stores excess iron in cells.

Several iron-related conditions can impact transferrin function including anemia and hemochromatosis. Anemia often occurs when there is insufficient iron delivery leading to inadequate hemoglobin synthesis and reduced oxygen transport. Aberrant transferrin receptor activity affects iron uptake in such conditions. Hemochromatosis characterized by iron overload can occur due to mutations in genes like HFE leading to changes in hepcidin regulation and increased intestinal iron absorption. Transferrin levels and saturation are clinical indicators used to assess iron status in such diseases.
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製品の性状

製品の状態

Lyophilized

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一般的な情報

機能

Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.. (Microbial infection) Serves as an iron source for Neisseria species, which capture the protein and extract its iron for their own use.. (Microbial infection) Serves as an iron source for parasite T.brucei (strain 427), which capture TF via its own transferrin receptor ESAG6 : ESAG7 and extract its iron for its own use.

配列の類似性

Belongs to the transferrin family.

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製品プロトコール

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ターゲットの情報

Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.. (Microbial infection) Serves as an iron source for Neisseria species, which capture the protein and extract its iron for their own use.. (Microbial infection) Serves as an iron source for parasite T.brucei (strain 427), which capture TF via its own transferrin receptor ESAG6 : ESAG7 and extract its iron for its own use.
See full target information TF
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関連製品

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