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AB157345

Recombinant human TRAIL protein

Recombinant human TRAIL protein

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(1 Publication)

Recombinant human TRAIL protein is a Human Fragment protein, in the 95 to 281 aa range, expressed in Escherichia coli, with >90%, < 0.1 EU/µg endotoxin level, suitable for SDS-PAGE, WB, FuncS.

別名を表示する

CD253, APO2L, TRAIL, TNFSF10, Tumor necrosis factor ligand superfamily member 10, Apo-2 ligand, TNF-related apoptosis-inducing ligand, Apo-2L, Protein TRAIL

2 Images
Western blot - Recombinant human TRAIL protein (AB157345)
  • WB

Lab

Western blot - Recombinant human TRAIL protein (AB157345)

All lanes:

Western blot - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [6F7] (<a href='/products/primary-antibodies/ddddk-tag-binds-to-flag-tag-sequence-antibody-6f7-ab213519'>ab213519</a>) at 1 µg/mL

Lanes 1 and 3:

Western blot - Recombinant human RANKL protein (Active) (<a href='/products/proteins-peptides/recombinant-human-rankl-protein-active-ab157289'>ab157289</a>) at 0.5 µg

Lanes 2 and 4:

Western blot - Recombinant human RANKL protein (Active) (<a href='/products/proteins-peptides/recombinant-human-rankl-protein-active-ab157289'>ab157289</a>) at 0.1 µg

Lane 3:

Western blot - Recombinant human TRAIL protein (ab157345) at 0.5 µg

Lane 4:

Western blot - Recombinant human TRAIL protein (ab157345) at 0.1 µg

Secondary

All lanes:

Goat Anti-Rat IgG H&L (IRDye® 800CW ) (<a href='/products/unavailable/goat-rat-igg-hl-irdye-800cw-ab253031'>ab253031</a>) at 1/20000 dilution

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SDS-PAGE - Recombinant human TRAIL protein (AB157345)
  • SDS-PAGE

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SDS-PAGE - Recombinant human TRAIL protein (AB157345)

SDS PAGE analysis of ab157345 (1μg).

Key facts

精製度

>90% SDS-PAGE

エンドトキシンレベル

< 0.1 EU/µg

発現系

Escherichia coli

タグ

DDDDK tag N-Terminus

アプリケーション

SDS-PAGE, FuncS, WB

applications

生物活性

Yes

生物学的活性

Active

アクセッション番号

P50591

アニマルフリー

No

キャリアフリー

Yes

種

Human

再構成

Reconstitute in 100 µL of water

バッファー組成

Constituents: PBS

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>(100ng, colorimetric).</p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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製品の詳細

Binds to Human and mouse TRAIL receptors and Human osteoprotegerin (OPG). Induces apoptosis in a concentration range of 1-100ng/ml if applied with a cross-linking enhancer.
Note: ab157345 does not significantly induce apoptosis in the absence of an enhancer.
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配列情報

[{"sequence":"TSEETISTVQEKQQNISPLVRERGPQRVAAHITGTRGRSNTLSSPNSKNEKALGRKINSWESSRSGHSFLSNLHLRNGELVIHEKGFYYIYSQTYFRFQEEIKENTKNDKQMVQYIYKYTSYPDPILLMKSARNSCWSKDAEYGLYSIYQGGIFELKENDRIFVSVTNEHLIDMDHEASFFGAFLVG","proteinLength":"Fragment","predictedMolecularWeight":"28 kDa","actualMolecularWeight":null,"aminoAcidEnd":281,"aminoAcidStart":95,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P50591","tags":[{"tag":"DDDDK","terminus":"N-Terminus"}]}]
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出荷温度及び保存条件

出荷温度
Blue Ice
短期保存温度
-20°C
長期保存温度
-20°C
True
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

TRAIL also known as TNF-related apoptosis-inducing ligand plays an important role in inducing apoptosis. This protein has a mass of around 32 kDa and is expressed across various tissues including immune cells. TRAIL interacts with specific receptors on the cell surface to initiate a signaling cascade that results in programmed cell death. It is an attractive therapeutic target because of its selective ability to induce apoptosis in cancer cells while sparing most normal cells.
Biological function summary

TRAIL's function extends beyond inducing cell death. It is an important member of the tumor necrosis factor (TNF) superfamily which regulates immune responses and inflammation. TRAIL can form a homotrimeric complex allowing it to bind effectively to death receptors on target cells specifically DR4 and DR5 triggering a series of downstream signaling events. This interaction is significant in modulating immune surveillance and tumor suppression.

Pathways

TRAIL is integral to both intrinsic and extrinsic apoptosis pathways. Through these pathways TRAIL is closely related to FADD and caspase-8 which are important for apoptosis induction. The extrinsic pathway involves binding to TRAIL death receptors leading to DISC formation while the intrinsic pathway involves mitochondrial events mediated by members of the Bcl-2 protein family.

TRAIL has a significant connection with cancer and autoimmune diseases. TRAIL plays a dual role in cancer where it acts as a tumor suppressor but can also promote tumor progression if cancer cells become resistant to its effects. In cancer TRAIL’s interaction with proteins like FLIP can alter the apoptotic potential of the cells. In autoimmune diseases TRAIL's dysregulation can contribute to tissue damage due to increased immune activity highlighting its complex role in maintaining immune balance.
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製品の性状

製品の状態

Lyophilized

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一般的な情報

機能

Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG (PubMed : 10549288, PubMed : 26457518). Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.

配列の類似性

Belongs to the tumor necrosis factor family.

翻訳後修飾

Tyrosine phosphorylated by PKDCC/VLK.

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製品プロトコール

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ターゲットの情報

Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG (PubMed : 10549288, PubMed : 26457518). Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.
See full target information TNFSF10
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of immunology research 2022:5582811 PubMed35600049

2022

A Substance P (SP)/Neurokinin-1 Receptor Axis Promotes Perineural Invasion of Pancreatic Cancer and Is Affected by lncRNA LOC389641.

Applications

Unspecified application

Species

Unspecified reactive species

Tengfei Ji,Keqiang Ma,Hongsheng Wu,Tiansheng Cao
View all publications
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