Recombinant Human TR11B Protein (Active) (ab287918)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Endotoxin level: <= 0.005 Eu/µg
- Active: Yes
- Suitable for: MS, Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant Human TR11B Protein (Active)
Osteoprotegerin タンパク質・ペプチド 製品一覧 -
生理活性
Fully biologically active determined by dose-dependent inhibition of RANKL-mediated NF-kb activation in mouse RAW264.7 NF-kb luciferase reporter cell line.
ED50 is ≤ 56.66, corresponding to a specific activity of 1.8 X 104 units/mg.
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精製度
>= 95 % SDS-PAGE. -
エンドトキシン・レベル
<=0.005 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
Yes -
キャリア・フリー
はい -
由来
Recombinant -
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生物種
Human -
配列
ETFPPKYLHYDEETSHQLLCDKCPPGTYLKQHCTAKWKTVCAPCPDHYYT DSWHTSDECLYCSPVCKELQYVKQECNRTHNRVCECKEGRYLEIEFCLKH RSCPPGFGVVQAGTPERNTVCKRCPDGFFSNETSSKAPCRKHTNCSVFGL LLTQKGNATHDNICSGNSESTQKCGIDVTLCEEAFFRFAVPTKFTPNWLS VLVDNLPGTKVNAESVERIKRQHSSQEQTFQLLKLWKHQNKDQDIVKKII QDIDLCENSVQRHIGHANLTFEQLRSLMESLPGKKVGAEDIEKTIKACKP SDQILKLLSLWRIKNGDQDTLKGLMHALKHSKTYHFPKTVTQSLKKTIRF LHSFTMYKLYQKLFLEMIGNQVQSVKISCL -
予測される分子量
44 kDa -
実際の分子量
44 kDa -
分子量情報
Mass determination by ESI-TOF. Predicted MW is 43674 Da (+/-10 Da by ESI-TOF). Observed MW is 43679.71 Da. -
領域
22 to 401 -
配列の追加情報
N-terminal glycine. Full-length mature chain lacking the signal peptide and pro-peptide.
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特性
Our Abpromise guarantee covers the use of ab287918 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Mass Spectrometry
Functional Studies
SDS-PAGE
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at Room Temperature. Store at Room Temperature.
pH: 7.4
Constituents: 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Monobasic dihydrogen potassium phosphate, 10.26% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Reconstitute with phosphate buffered saline. Store lyophilized form at room temperature. Reconstitute, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw. Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product.
関連情報
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別名
- MGC29565
- OCIF
- OPG
see all -
機能
Acts as decoy receptor for RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local RANKL/OPG ratio. May also play a role in preventing arterial calcification. May act as decoy receptor for TRAIL and protect against apoptosis. TRAIL binding blocks the inhibition of osteoclastogenesis. -
組織特異性
Highly expressed in adult lung, heart, kidney, liver, spleen, thymus, prostate, ovary, small intestine, thyroid, lymph node, trachea, adrenal gland, testis, and bone marrow. Detected at very low levels in brain, placenta and skeletal muscle. Highly expressed in fetal kidney, liver and lung. -
関連疾患
Defects in TNFRSF11B are the cause of juvenile Paget disease (JPD) [MIM:239000]; also known as hyperostosis corticalis deformans juvenilis or hereditary hyperphosphatasia or chronic congenital idiopathic hyperphosphatasia. JPD is a rare autosomal recessive osteopathy that presents in infancy or early childhood. The disorder is characterized by rapidly remodeling woven bone, osteopenia, debilitating fractures, and deformities due to a markedly accelerated rate of bone remodeling throughout the skeleton. Approximately 40 cases of JPD have been reported worldwide. Unless it is treated with drugs that block osteoclast-mediated skeletal resorption, the disease can be fatal. -
配列類似性
Contains 2 death domains.
Contains 4 TNFR-Cys repeats. -
翻訳後修飾
N-glycosylated. Contains sialic acid residues.
The N-terminus is blocked. -
細胞内局在
Secreted. - Information by UniProt
画像
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Mass Spectrometry - Recombinant Human Osteoprotegerin Protein (Active) (ab287918)
Mass determination by ESI-TOF. Predicted MW is 43674 Da (+/-10 Da by ESI-TOF). Observed MW is 43679.71 Da.
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Functional Studies - Recombinant Human Osteoprotegerin Protein (Active) (ab287918)Fully biologically active determined by dose-dependent inhibition of RANKL-mediated NF-kb activation in mouse RAW264.7 NF-kb luciferase reporter cell line.
ED50 is ≤ 56.66, corresponding to a specific activity of 1.8 X 104 units/mg.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot. Lot GR3432994-1.
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SDS-PAGE - Recombinant Human Osteoprotegerin Protein (ab287918)SDS-PAGE analysis of ab287918
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab287918 は論文での使用が確認できていません。
