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Recombinant Human Topoisomerase I (mutated Y723F) protein (ab82093)

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Key features and details

  • Expression system: Baculovirus infected insect cells
  • Purity: > 95% SDS-PAGE
  • Suitable for: SDS-PAGE

製品の詳細

  • 製品名

    Recombinant Human Topoisomerase I (mutated Y723F) protein
    Topoisomerase I タンパク質・ペプチド 製品一覧

  • 精製度

    > 95 % SDS-PAGE.
    Immunogen affinity purified, purity determined by SDS-PAGE

  • 発現系

    Baculovirus infected insect cells

  • タンパク質長

    Full length protein

  • Animal free

    No

  • 由来

    Recombinant

    • 生物種

      Human

    • 修飾

      mutated Y723F

特性

Our Abpromise guarantee covers the use of ab82093 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    SDS-PAGE

  • 製品の状態

    Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.9
    Constituents: 0.75% Potassium chloride, 0.0154% DTT, 0.316% Tris HCl, 0.00584% EDTA, 20% Glycerol (glycerin, glycerine)

関連情報

  • 別名

    • DNA topoisomerase 1
    • DNA topoisomerase I
    • NUP98 fusion gene
    • TOP 1
    • TOP I
    • TOP1
    • TOP1_HUMAN
    • TOPI
    • Topoisomerase (DNA) I
    • Topoisomerase 1
    • Topoisomerase1
    • TopoisomeraseI
    • Type I DNA topoisomerase
    see all

  • 機能

    The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another.

  • 関連疾患

    Note=A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with NUP98.

  • 配列類似性

    Belongs to the eukaryotic type I topoisomerase family.

  • 翻訳後修飾

    Sumoylated. Lys-117 is the main site of sumoylation. Sumoylation plays a role in partitioning TOP1 between nucleoli and nucleoplasm. Levels are dramatically increased on camptothecin (CPT) treatment.

  • 細胞内局在

    Nucleus > nucleolus. Nucleus > nucleoplasm. Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumolyated forms found in both nucleoplasm and nucleoli.
  • Information by UniProt

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab82093 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab82093 は論文での使用が確認できていません。

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