Recombinant human TNF alpha protein (ab52126)
Key features and details
- Expression system: Escherichia coli
- Purity: >= 98% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
Achieve higher consistency and quality standards with a premium grade bioactive protein
- High batch-to-batch consistency
- Optimal bioactivity
- Guaranteed identical to human native proteins
- >95% purity
- Ultra-low endotoxin levels: <0.005 Eu/µg
- Carrier and tag free
製品の詳細
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製品名
Recombinant human TNF alpha protein
TNF alpha タンパク質・ペプチド 製品一覧 -
生理活性
ED50 < 0.05 ng/ml as determined by the cytolysis of murine L929 cells in the presence of actinomycin D.
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精製度
>= 98 % SDS-PAGE.
>98% by SDS-PAGE and HPLC analyses -
発現系
Escherichia coli -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
Yes -
由来
Recombinant -
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生物種
Human -
配列
VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVV PSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSP CQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQV YFGIIAL -
予測される分子量
17 kDa -
実際の分子量
17 kDa
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特性
Our Abpromise guarantee covers the use of ab52126 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Functional Studies
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store under desiccating conditions.
pH: 8.50
Constituents: 0.606% Tris, 0.877% Sodium chloride
Lyophilized in 50 mM Tris, 150 mM NaCl pH 8.5This product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Reconstitute in H2O to a concentration of 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use.
関連情報
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別名
- APC1
- APC1 protein
- Cachectin
see all -
機能
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. -
関連疾患
Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). -
配列類似性
Belongs to the tumor necrosis factor family. -
翻訳後修飾
The soluble form derives from the membrane form by proteolytic processing.
The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
画像
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Functional Studies - Recombinant human TNF alpha protein (ab52126)
Fig. B. Gel filtration analysis of ab52126: SEC analysis of ab52126 on a Superose 6 Increase 5/150 column at 0.1mL/min in in 50 mM Na-phosphate, 300mM NaCl pH 7.2
Fig. C. ED50 of ab52126: ED50 < 0.05 ng/ml as determined by the cytolysis of murine L929 cells treated with actinomycin using Quick Cell Proliferation Colorimetric Assay Kit Plus
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SDS-PAGE - Recombinant human TNF alpha protein (ab52126)SDS-PAGE (4-20%) of Recombinant human TNF-α Protein loaded under reducing conditions and stained with Coomassie Blue. Lanes are as follows: M - MW markers, 1, 3 and 5 µg purified TNF-α. The protein shows a predicted MW of ~ 17.4 kDa
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab52126 は論文での使用が確認できていません。
