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AB89855

Recombinant human TLK2 protein

Recombinant human TLK2 protein

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Recombinant human TLK2 protein is a Human Fragment protein, in the 388 to 772 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, WB, FuncS.

別名を表示する

Serine/threonine-protein kinase tousled-like 2, HsHPK, PKU-alpha, Tousled-like kinase 2, TLK2

4 Images
Functional Studies - Recombinant human TLK2 protein (AB89855)
  • FuncS

Unknown

Functional Studies - Recombinant human TLK2 protein (AB89855)

Kinase Assay demonstrating specific activity of ab89855

Functional Studies - Recombinant human TLK2 protein (AB89855)
  • FuncS

Unknown

Functional Studies - Recombinant human TLK2 protein (AB89855)

The specific activity of TLK2 (ab89855) was determined to be 19 nmol/min/mg as per activity assay protocol and was equivalent to 85 nmol/min/mg as per radiometric assay

SDS-PAGE - Recombinant human TLK2 protein (AB89855)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human TLK2 protein (AB89855)

SDS-PAGE showing ab89855 at approximately 67kDa

SDS-PAGE - Recombinant human TLK2 protein (AB89855)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human TLK2 protein (AB89855)

SDS PAGE analysis of ab89855

Key facts

精製度

>90% SDS-PAGE

発現系

Baculovirus infected Sf9 cells

タグ

GST tag N-Terminus

アプリケーション

SDS-PAGE, WB, FuncS

applications

生物活性

Yes

生物学的活性

The specific activity of ab89855 was determined to be 79 nmol/min/mg

アクセッション番号

Q86UE8

アニマルフリー

No

キャリアフリー

No

種

Human

バッファー組成

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0038% EGTA, 0.00292% EDTA, 0.00174% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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製品の詳細

ab91092 (Cow Casein full length protein) can be utilized as a substrate for assessing Kinase activity
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配列情報

[{"linker":null,"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":772,"aminoAcidStart":388,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q86UE8","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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出荷温度及び保存条件

出荷温度
Dry Ice
短期保存温度
-80°C
長期保存温度
-80°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
True
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

TLK2 also known as Tousled-like kinase 2 plays a role as a serine/threonine kinase responsible for phosphorylating proteins involved in chromatin remodeling. It has a molecular mass of approximately 90 kDa. TLK2 expresses mainly in proliferative tissues like testes and ovaries highlighting its involvement in cell proliferation processes. This kinase also shows up in other tissues where cell division is active assisting in DNA repair and cell cycle control mechanisms.
Biological function summary

TLK2 acts as a modulator of chromatin assembly by interacting with the ASF1 proteins forming a critical complex in cellular processes. Its activity is essential for the proper progression of the S-phase during the cell cycle. This function connects TLK2 to the maintenance of genomic stability largely through regulating histone incorporation. Such interactions are necessary for the preservation of the genomic integrity during DNA replication.

Pathways

TLK2 significantly impacts cell cycle regulation and DNA damage response pathways. Its participation in the cell cycle pathway intersecting with other kinases is evident as it influences the activity of proteins like CHK1 impacting the G2/M checkpoint. TLK2's involvement in the DNA damage response closely related to the ATM/ATR signaling pathway positions it as an essential player in responding to DNA insults and maintaining cell survival during stress.

TLK2 links closely with cancer especially involving ovarian and testicular tumors where aberrant expression or mutations contribute to uncontrolled cell proliferation. Mutations in TLK2 also have associations with developmental disorders influencing proteins like ASF1B with potential impacts on cognitive development. Understanding TLK2's role in these contexts provides essential insights into potential therapeutic strategies targeting dysregulated cellular processes.
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製品の性状

製品の状態

Liquid

補足情報

Affinity purified.

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一般的な情報

機能

Serine/threonine-protein kinase involved in the process of chromatin assembly and probably also DNA replication, transcription, repair, and chromosome segregation (PubMed : 10523312, PubMed : 11470414, PubMed : 12660173, PubMed : 12955071, PubMed : 29955062, PubMed : 33323470, PubMed : 9427565). Phosphorylates the chromatin assembly factors ASF1A and ASF1B (PubMed : 11470414, PubMed : 20016786, PubMed : 29955062, PubMed : 35136069). Phosphorylation of ASF1A prevents its proteasome-mediated degradation, thereby enhancing chromatin assembly (PubMed : 20016786). Negative regulator of amino acid starvation-induced autophagy (PubMed : 22354037).

配列の類似性

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

翻訳後修飾

Phosphorylated at Ser-750, probably by CHEK1.. Autophosphorylated; phosphorylation promotes the assembly of higher order oligomers and enzymatic activity.

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製品プロトコール

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ターゲットの情報

Serine/threonine-protein kinase involved in the process of chromatin assembly and probably also DNA replication, transcription, repair, and chromosome segregation (PubMed : 10523312, PubMed : 11470414, PubMed : 12660173, PubMed : 12955071, PubMed : 29955062, PubMed : 33323470, PubMed : 9427565). Phosphorylates the chromatin assembly factors ASF1A and ASF1B (PubMed : 11470414, PubMed : 20016786, PubMed : 29955062, PubMed : 35136069). Phosphorylation of ASF1A prevents its proteasome-mediated degradation, thereby enhancing chromatin assembly (PubMed : 20016786). Negative regulator of amino acid starvation-induced autophagy (PubMed : 22354037).
See full target information TLK2
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