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AB125563

Recombinant ヒト TLK1 protein (GST tag N-Terminus)

Recombinant human TLK1 protein (GST tag N-Terminus)

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Recombinant human TLK1 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 766 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, WB, FuncS.

別名を表示する

KIAA0137, TLK1, Serine/threonine-protein kinase tousled-like 1, PKU-beta, Tousled-like kinase 1

4 Images
Functional Studies - Recombinant human TLK1 protein (GST tag N-Terminus) (AB125563)
  • FuncS

Unknown

Functional Studies - Recombinant human TLK1 protein (GST tag N-Terminus) (AB125563)

Sample Kinase Assay showing the specific activity of ab125563 to be 6.2 nmol/min/mg.

Functional Studies - Recombinant human TLK1 protein (GST tag N-Terminus) (AB125563)
  • FuncS

Unknown

Functional Studies - Recombinant human TLK1 protein (GST tag N-Terminus) (AB125563)

The specific activity of TLK1 (ab125563) was determined to be 7.1 nmol/min/mg as per activity assay protocol

SDS-PAGE - Recombinant human TLK1 protein (GST tag N-Terminus) (AB125563)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human TLK1 protein (GST tag N-Terminus) (AB125563)

SDS PAGE analysis of ab125563

SDS-PAGE - Recombinant human TLK1 protein (GST tag N-Terminus) (AB125563)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human TLK1 protein (GST tag N-Terminus) (AB125563)

SDS-PAGE analysis of ab125563.

Key facts

精製度

>90% Densitometry

Purity determined to be >90% by densitometry.

発現系

Baculovirus infected Sf9 cells

タグ

GST tag N-Terminus

アプリケーション

SDS-PAGE, FuncS, WB

applications

生物活性

Yes

生物学的活性

The specific activity of ab125563 was determined to be 6.2 - 7.1 nmol/min/mg.

アクセッション番号

Q9UKI8

アニマルフリー

No

キャリアフリー

No

種

Human

バッファー組成

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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組合せ製品

Proteins & Peptides

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Recombinant Human KAP1 protein (denatured) (His tag N-Terminus)

SDS-PAGE - Recombinant Human KAP1 protein (denatured) (His tag N-Terminus) (AB171679)

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Mass Spectrometry - Recombinant Human CXCL1/GRO alpha protein (AB283439)

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SDS-PAGE - Recombinant Human SIRPB1 protein (His tag C-Terminus) (AB235876)

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Proteins & Peptides

AB97948

Recombinant E. coli Nitroreductase protein (His tag N-Terminus)

SDS-PAGE - Recombinant E. coli Nitroreductase protein (His tag N-Terminus) (AB97948)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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配列情報

[{"linker":null,"sequence":"MSVQSSSGSLEGPPSWSQLSTSPTPGSAAAARSLLNHTPPSGRPREGAMDELHSLDPRRQELLEARFTGVASGSTGSTGSCSVGAKASTNNESSNHSFGSLGSLSDKESETPEKKQSESSRGRKRKAENQNESSQGKSIGGRGHKISDYFEYQGGNGSSPVRGIPPAIRSPQNSHSHSTPSSSVRPNSPSPTALAFGDHPIVQPKQLSFKIIQTDLTMLKLAALESNKIQDLEKKEGRIDDLLRANCDLRRQIDEQQKLLEKYKERLNKCISMSKKLLIEKSTQEKLSSREKSMQDRLRLGHFTTVRHGASFTEQWTDGFAFQNLVKQQEWVNQQREDIERQRKLLAKRKPPTANNSQAPSTNSEPKQRKNKAVNGAENDPFVRPNLPQLLTLAEYHEQEEIFKLRLGHLKKEEAEIQAELERLERVRNLHIRELKRINNEDNSQFKDHPTLNERYLLLHLLGRGGFSEVYKAFDLYEQRYAAVKIHQLNKSWRDEKKENYHKHACREYRIHKELDHPRIVKLYDYFSLDTDTFCTVLEYCEGNDLDFYLKQHKLMSEKEARSIVMQIVNALRYLNEIKPPIIHYDLKPGNILLVDGTACGEIKITDFGLSKIMDDDSYGVDGMDLTSQGAGTYWYLPPECFVVGKEPPKISNKVDVWSVGVIFFQCLYGRKPFGHNQSQQDILQENTILKATEVQFPVKPVVSSEAKAFIRRCLAYRKEDRFDVHQLANDPYLLPHMRRSNSSGNLHMAGLTASPTPPSSSIITY","proteinLength":"Full Length","predictedMolecularWeight":"135 kDa","actualMolecularWeight":null,"aminoAcidEnd":766,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q9UKI8","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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出荷温度及び保存条件

製品の状態
Liquid
出荷温度
Dry Ice
短期保存温度
-80°C
長期保存温度
-80°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
True
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Toutatis-like kinase 1 (TLK1) also known as serine/threonine-protein kinase tousled-like 1 is a kinase that plays a role in DNA repair and chromatin assembly. Its molecular mass is approximately 85 kDa. It is widely expressed in various human tissues with notable expression in the testis and brain. TLK1 primarily functions during DNA replication where it phosphorylates substrates that are vital for maintaining genomic stability.
Biological function summary

TLK1 contributes significantly to chromatin remodeling and DNA repair by influencing histone dynamics. TLK1 often acts in concert with other proteins as part of regulatory complexes modulating DNA accessibility during replication and repair processes. As a kinase it phosphorylates and activates targets like Asf1 a histone chaperone impacting nucleosome assembly.

Pathways

TLK1 plays a significant role in the DNA damage response and cell cycle regulation pathways. It is closely related to the ATM/ATR pathway which is central to the cellular response to DNA damage. TLK1 also interacts with proteins like Asf1 influencing replication-coupled chromatin assembly and cell cycle progression by modifying histones and other structural proteins.

TLK1 has been linked to certain cancers and neurodegenerative disorders. In cancer disruptions in TLK1 activity can lead to genomic instability contributing to tumorigenesis. TLK1 also connects with proteins like ASF1A in these contexts affecting cell proliferation and survival. In neurodegenerative diseases alterations in TLK1 function might impact neurological pathways related to DNA repair though specific mechanisms remain under investigation.
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一般的な情報

機能

Rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly. Isoform 3 phosphorylates and enhances the stability of the t-SNARE SNAP23, augmenting its assembly with syntaxin. Isoform 3 protects the cells from the ionizing radiation by facilitating the repair of DSBs. In vitro, phosphorylates histone H3 at 'Ser-10'.

配列の類似性

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

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製品プロトコール

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ターゲットの情報

Rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly. Isoform 3 phosphorylates and enhances the stability of the t-SNARE SNAP23, augmenting its assembly with syntaxin. Isoform 3 protects the cells from the ionizing radiation by facilitating the repair of DSBs. In vitro, phosphorylates histone H3 at 'Ser-10'.
See full target information TLK1
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