AB92633

Recombinant human Tissue Plasminogen Activator protein, Single Chain (Active)

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(1 Publication)

Recombinant human Tissue Plasminogen Activator protein, Single Chain (Active) is a Human Full Length protein, in the 1 to 562 aa range, expressed in CHO cells, with >95%, suitable for SDS-PAGE, FuncS.

別名を表示する

Tissue-type plasminogen activator, t-PA, t-plasminogen activator, tPA, PLAT

1 Images

SDS-PAGE - Recombinant human Tissue Plasminogen Activator protein, Single Chain (Active) (AB92633)

SDS-PAGE

Unknown

SDS-PAGE - Recombinant human Tissue Plasminogen Activator protein, Single Chain (Active) (AB92633)

3 ug of ab92633 on SDS-PAGE.

Lane 1 : Non-reducing.

Lane 2 : Reducing.

Key facts

精製度

>95% SDS-PAGE

発現系

CHO cells

アプリケーション

FuncS, SDS-PAGE

applications

生物活性

Yes

生物学的活性

Specific activity: 593,600 IU/mg (Relative to WHO International Standard for Human Recombinant Tissue Plasminogen Activator, NIBSC 98/714).

アクセッション番号

P00750

アニマルフリー

キャリアフリー

種

Human

バッファー組成

pH: 7.4 Constituents: 9.52% HEPES, 0.58% Sodium chloride

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Human tissue-type plasminogen activator (tPA) is a 527 amino acid glycoprotein. Synthesized from cDNA from a human melanoma cell line.

>85% single chain.

Extinction coefficient: 1.9

Ultraviolet Absorbance (280nm) = 6.27

Fully complexable with human PAI-1.

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配列情報

[{"sequence":"MDAMKRGLCCVLLLCGAVFVSPSQEIHARFRRGARSYQVICRDEKTQMIYQQHQSWLRPVLRSNRVEYCWCNSGRAQCHSVPVKSCSEPRCFNGGTCQQALYFSDFVCQCPEGFAGKCCEIDTRATCYEDQGISYRGTWSTAESGAECTNWNSSALAQKPYSGRRPDAIRLGLGNHNYCRNPDRDSKPWCYVFKAGKYSSEFCSTPACSEGNSDCYFGNGSAYRGTHSLTESGASCLPWNSMILIGKVYTAQNPSAQALGLGKHNYCRNPDGDAKPWCHVLKNRRLTWEYCDVPSCSTCGLRQYSQPQFRIKGGLFADIASHPWQAAIFAKHRRSPGERFLCGGILISSCWILSAAHCFQERFPPHHLTVILGRTYRVVPGEEEQKFEVEKYIVHKEFDDDTYDNDIALLQLKSDSSRCAQESSVVRTVCLPPADLQLPDWTECELSGYGKHEALSPFYSERLKEAHVRLYPSSRCTSQHLLNRTVTDNMLCAGDTRSGGPQANLHDACQGDSGGPLVCLNDGRMTLVGIISWGLGCGQKDVPGVYTKVTNYLDWIRDNMRP","proteinLength":"Full Length","predictedMolecularWeight":"70 kDa","actualMolecularWeight":null,"aminoAcidEnd":562,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"CHO cells","accessionNumber":"P00750","tags":[]}]

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出荷温度及び保存条件

出荷温度

Dry Ice

短期保存温度

-80°C

長期保存温度

-80°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

True

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Tissue Plasminogen Activator (tPA) also known as a plasminogen activator acts mechanically to convert plasminogen to plasmin a serine protease. This reaction occurs at the surface of a fibrin clot leading to clot degradation a process known as fibrinolysis. tPA has a molecular mass of approximately 70 kDa. It is mainly expressed in vascular endothelial cells and is released into the bloodstream in response to stimuli such as circulatory stasis or endothelial damage.

Biological function summary

TPA plays a critical role in thrombolysis by breaking down blood clots into their soluble components. It regulates plasminogen function by cleaving this zymogen to yield the active protease plasmin. This function makes tPA integral in maintaining hemostasis and it does not form a part of a larger protein complex. The activity and the regulation of tPA are important for preventing pathologic clotting which can lead to cardiovascular complications.

Pathways

TPA is central to the fibrinolytic pathway. This pathway facilitates the conversion of plasminogen to plasmin enabling clot resolution. In addition tPA interacts with other proteins such as urokinase plasminogen activator (uPA) and inhibitors like plasminogen activator inhibitor-1 (PAI-1). The balance between tPA and its inhibitors is important for the regulation of fibrinolytic activity impacting hemostatic and thrombotic events.

TPA connects closely with conditions like stroke and myocardial infarction due to its thrombolytic properties. In ischemic stroke excessive or insufficient tPA activity can disrupt normal blood flow leading to tissue damage. Additionally in myocardial infarction tPA's role in breaking down clots proves important for restoring coronary blood flow. It is also linked with proteins like fibrinogen as they serve as substrates in the clot degradation process and with PAI-1 which modulates its activity and influences disease progression.

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製品の性状

製品の状態

Liquid

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一般的な情報

機能

Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. During oocyte activation, plays a role in cortical granule reaction in the zona reaction, which contributes to the block to polyspermy (By similarity).

配列の類似性

Belongs to the peptidase S1 family.

翻訳後修飾

The single chain, almost fully active enzyme, can be further processed into a two-chain fully active form by a cleavage after Arg-310 catalyzed by plasmin, tissue kallikrein or factor Xa.. Differential cell-specific N-linked glycosylation gives rise to two glycoforms, type I (glycosylated at Asn-219) and type II (not glycosylated at Asn-219). The single chain type I glycoform is less readily converted into the two-chain form by plasmin, and the two-chain type I glycoform has a lower activity than the two-chain type II glycoform in the presence of fibrin.. N-glycosylation of Asn-152; the bound oligomannosidic glycan is involved in the interaction with the mannose receptor.. Characterization of O-linked glycan was studied in Bowes melanoma cell line.

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information PLAT

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 9: PubMed38502232

2024

Dysregulated fibrinolysis and plasmin activation promote the pathogenesis of osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Wang,Guoqiang Shao,Xiaoyi Zhao,Heidi H Wong,Kate Chin,Mackenzie Zhao,Audrey Bai,Michelle S Bloom,Zelda Z Love,Constance R Chu,Zhen Cheng,William H Robinson

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Recombinant human Tissue Plasminogen Activator protein, Single Chain (Active)