Recombinant Human TIMP2 Protein (Active) (ab291057)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus
製品の詳細
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製品名
Recombinant Human TIMP2 Protein (Active)
TIMP2 タンパク質・ペプチド 製品一覧 -
生理活性
Fully active determined by its ability to inhibit human MMP2 cleavage of a chromogenic peptide substrate (Mca-PLGLDpa-AR-NH2).
ED50 is ≤ 4.9 ng/ml, corresponding to a specific activity of 2.0 x 105 units/mg.
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精製度
>= 95 % SDS-PAGE. -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
Yes -
由来
Recombinant -
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生物種
Human -
配列
CSCSPVHPQQAFCNADVVIRAKAVSEKEVDSGNDIYGNPIKRIQYEIKQI KMFKGPEKDIEFIYTAPSSAVCGVSLDVGGKKEYLIAGKAEGDGKMHITL CDFIVPWDTLSTTQKKSLNHRYQMGCECKITRCPMIPCYISSPDECLWMD WVTEKNINGHQAKFFACIKRSDGSCAWYRGAAPPKQEFLDIEDP -
予測される分子量
24 kDa -
領域
27 to 220 -
タグ
His tag C-Terminus
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製品の詳細
Recombinant Human TIMP2 Protein
特性
Our Abpromise guarantee covers the use of ab291057 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at Room Temperature. Store at Room Temperature.
pH: 7.40
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Monobasic dihydrogen potassium phosphateThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Store lyophilized form at room temperature. Reconstitute in PBS, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
関連情報
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別名
- CSC 21K
- CSC-21K
- CSC21K
see all -
機能
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19. -
配列類似性
Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain. -
翻訳後修飾
The activity of TIMP2 is dependent on the presence of disulfide bonds. -
細胞内局在
Secreted. - Information by UniProt
画像
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Biological Activity - Recombinant Human TIMP2 Protein (Active) (ab291057)
Fully active determined by its ability to inhibit human MMP2 cleavage of a chromogenic peptide substrate (Mca-PLGLDpa-AR-NH2).
ED50 is ≤ 4.9 ng/ml, corresponding to a specific activity of 2.0 x 105 units/mg.
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SDS-PAGE - Recombinant Human TIMP2 Protein (Active) (ab291057)SDS-PAGE analysis of ab291057
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab291057 は論文での使用が確認できていません。
