AB291057

Recombinant Human TIMP2 Protein (Active)

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Recombinant Human TIMP2 Protein (Active) is a Human Full Length protein, in the 27 to 220 aa range, with >95%, suitable for FuncS.

別名を表示する

Metalloproteinase inhibitor 2, CSC-21K, Tissue inhibitor of metalloproteinases 2, TIMP-2, TIMP2

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Mass Spectrometry - Recombinant Human TIMP2 Protein (Active) (AB291057)

Mass Spec

Supplier Data

Mass Spectrometry - Recombinant Human TIMP2 Protein (Active) (AB291057)

Biological Activity - Recombinant Human TIMP2 Protein (Active) (AB291057)

Biological Activity

Supplier Data

Biological Activity - Recombinant Human TIMP2 Protein (Active) (AB291057)

Fully active determined by its ability to inhibit human MMP2 cleavage of a chromogenic peptide substrate (Mca-PLGLDpa-AR-NH2). ED50 is ≤ 4.9 ng/ml, corresponding to a specific activity of 2.0 x 105 units/mg.

SDS-PAGE - Recombinant Human TIMP2 Protein (Active) (AB291057)

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human TIMP2 Protein (Active) (AB291057)

SDS-PAGE analysis of ab291057

Key facts

精製度

>95% SDS-PAGE

アプリケーション

FuncS

applications

生物活性

Yes

生物学的活性

Fully active determined by its ability to inhibit human MMP2 cleavage of a chromogenic peptide substrate (Mca-PLGLDpa-AR-NH2).

ED50 is ≤ 4.9 ng/ml, corresponding to a specific activity of 2.0 x 105 units/mg.

アクセッション番号

P16035

アニマルフリー

キャリアフリー

種

Human

再構成

Reconstitute in PBS

バッファー組成

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" } } }

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配列情報

[{"sequence":"CSCSPVHPQQAFCNADVVIRAKAVSEKEVDSGNDIYGNPIKRIQYEIKQIKMFKGPEKDIEFIYTAPSSAVCGVSLDVGGKKEYLIAGKAEGDGKMHITLCDFIVPWDTLSTTQKKSLNHRYQMGCECKITRCPMIPCYISSPDECLWMDWVTEKNINGHQAKFFACIKRSDGSCAWYRGAAPPKQEFLDIEDP","proteinLength":"Full Length","predictedMolecularWeight":"21.81 kDa","actualMolecularWeight":"21.81 kDa","aminoAcidEnd":220,"aminoAcidStart":27,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P16035","tags":[]}]

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出荷温度及び保存条件

出荷温度

Ambient - Can Ship with Ice

短期保存温度

Ambient

長期保存温度

Ambient

True

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

TIMP2 also known as Tissue Inhibitor of Metalloproteinases 2 mechanistically inhibits the activity of matrix metalloproteinases (MMPs). It binds to MMPs regulating the extracellular matrix's remodeling. The protein has a molecular mass of around 21 kDa. TIMP2 is expressed in various tissues including the brain liver and kidneys where it maintains a balance between matrix degradation and production.

Biological function summary

TIMP2 plays a significant role in tissue maintenance and repair by controlling extracellular matrix turnover. It forms complexes with MMPs such as MMP-2 inhibiting their proteolytic activities. The protein is also involved in cell proliferation and differentiation affecting processes like angiogenesis and wound healing. Its regulatory function aids in maintaining tissue homeostasis and preventing excessive matrix breakdown.

Pathways

TIMP2 is integral to the matrix metalloproteinase pathway and the regulation of endothelial cell migration. The protein interacts closely with other MMPs influencing their activation and modulation. Through these pathways TIMP2 holds an important position in cellular migration and signaling ensuring precise matrix remodeling in response to physiological needs.

TIMP2 has significant links with cancer and fibrosis. Dysregulation of TIMP2 expression or its imbalance with MMPs can contribute to tumor progression and metastasis due to uncontrolled matrix degradation. Additionally abnormal TIMP2 levels associate with fibrotic diseases where excessive tissue deposition occurs. TIMP2's interaction with proteins like MMP-9 and MMP-2 highlights its involvement in these pathological conditions.

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製品の性状

製品の状態

Lyophilized

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一般的な情報

機能

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.

配列の類似性

Belongs to the protease inhibitor I35 (TIMP) family.

翻訳後修飾

The activity of TIMP2 is dependent on the presence of disulfide bonds.

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information TIMP2

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Recombinant Human TIMP2 Protein (Active)