AB275556

Recombinant Human Thyroid Peroxidase/TPO protein (His tag)

Name: Recombinant Human Thyroid Peroxidase/TPO protein (His tag) (ab275556) | Abcam
Brand: Abcam Limited
SKU: AB275556
Price: 107000 JPY
Availability: InStock

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Recombinant Human Thyroid Peroxidase/TPO protein (His tag) is a Human Fragment protein, in the 7 to 220 aa range, expressed in Escherichia coli, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE.

別名を表示する

Thyroid peroxidase, TPO

1 Images

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Thyroid Peroxidase/TPO protein (His tag) (AB275556)

SDS-PAGE analysis of ab275556, 15% gel.

Key facts

精製度

>95% SDS-PAGE

エンドトキシンレベル

< 1 EU/µg

発現系

Escherichia coli

アプリケーション

SDS-PAGE

applications

生物活性

アクセッション番号

P07202

アニマルフリー

キャリアフリー

種

Human

再構成

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

バッファー組成

pH: 8 Constituents: PBS, 82.8% Trehalose, 0.17% Sodium-N-Lauroylsarcosinate

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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配列情報

[{"sequence":"LSVTLVMACTEAFFPFISRGKELLWGKPEESRVSSVLEESKRLVDTAMYATMQRNLKKRGILSPAQLLSFSKLPEPTSGVIARAAEIMETSIQAMKRKVNLKTQQSQHPTDALSEDLLSIIANMSGCLPYMLPPKCPNTCLANKYRPITGACNNRDHPRWGASNTALARWLPPVYEDGFSQPRGWNPGFLYNGFPLPPVREVTRHVIQVSNEVV","proteinLength":"Fragment","predictedMolecularWeight":"25.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":220,"aminoAcidStart":7,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P07202","tags":[{"tag":"His","terminus":"N-Terminus"}]}]

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-80°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

False

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Thyroid Peroxidase (TPO) also known as thyroperoxidase is an enzyme with a molecular mass of around 105 kDa. It is expressed in the thyroid gland's follicular cells. TPO plays a mechanical role in the synthesis of thyroid hormones by catalyzing iodination and coupling reactions needed to produce triiodothyronine (T3) and thyroxine (T4). These hormones are essential for regulating metabolism growth and development. The activity of TPO is an important step in thyroid hormone production.

Biological function summary

TPO participates in the thyroid's hormone biosynthesis pathway. It forms a part of the multi-enzyme complex that includes thyroglobulin and iodide. The enzyme facilitates the iodination of tyrosine residues on thyroglobulin and the coupling of iodotyrosines important for creating active thyroid hormones. TPO's enzymatic function is important for maintaining appropriate levels of circulating T3 and T4 impacting various physiological processes in the body.

Pathways

TPO contributes to the thyroid hormone synthesis pathway which is a critical pathway for metabolic regulation. It works in close association with other proteins such as thyroglobulin and sodium/iodide symporter (NIS) to ensure efficient thyroid hormone production. TPO's involvement in these pathways indicates its importance in the endocrine system's normal function and homeostasis.

TPO is often associated with autoimmune thyroid diseases like Hashimoto's thyroiditis and Graves' disease. High TPO antibodies such as anti-TPO antibodies are commonly detected in these conditions indicating an autoimmune response against the thyroid. The presence of high anti-thyroid peroxidase antibodies can lead to the destruction of thyroid tissue often resulting in hypothyroidism. TPO's connection to these diseases highlights its role in autoimmune conditions affecting thyroid function.

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製品の性状

製品の状態

Lyophilized

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一般的な情報

機能

Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4).

配列の類似性

Belongs to the peroxidase family. XPO subfamily.

翻訳後修飾

Glycosylated.. Heme is covalently bound through a H(2)O(2)-dependent autocatalytic process. Heme insertion is important for the delivery of protein at the cell surface.. Cleaved in its N-terminal part.

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4).

See full target information Thyroid peroxidase

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Abcam product promise

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Recombinant Human Thyroid Peroxidase/TPO protein (His tag)