Recombinant human TGF beta 1 protein (Active) (ab50036)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 98% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: HPLC, Cell Culture, SDS-PAGE, Functional Studies, WB
製品の詳細
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製品名
Recombinant human TGF beta 1 protein (Active)
TGF beta 1 タンパク質・ペプチド 製品一覧 -
生理活性
The ED50, as determined by TGF-beta1's ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells, is ≤ 0.05 ng/ml), corresponding to a specific activity of ≥ 2 x 107 units/mg.
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精製度
> 98 % SDS-PAGE.
> 98% by HPLC analyses. Endotoxin Level: < 0.1 ng/µg of protein (< 1 EU/µg). -
エンドトキシン・レベル
< 1.000 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS -
予測される分子量
25 kDa -
領域
279 to 390 -
配列の追加情報
TGF-beta1 is a 25.0 kDa protein with each subunit containing 112 aa, linked by a single disulfide bond. It is the mature protein after cleavage of the signal peptide and latency-associated peptide.
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特性
Our Abpromise guarantee covers the use of ab50036 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
HPLC
Cell Culture
SDS-PAGE
Functional Studies
Western blot
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製品の状態
Lyophilized -
備考
TGF-beta is secreted predominantly as latent complexes which are stored at the cell surface and in the extracellular matrix.
The release of biologically active TGF-β isoform from a latent complex involves proteolytic processing of the complex and /or induction of conformational changes by proteins such as thrombospondin-1.
TGF-β1 is the most abundant isoform secreted by almost every cell type.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Initially reconstitute in 10mM Citric Acid, pH 3.0 to 0.1-1.0 mg/ml. Store at 2°C to 8°C for up to 1 week or prepare for extended storage. After initial reconstitution, further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA). Store working aliquots at -20°C to -80°C.
関連情報
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別名
- Cartilage-inducing factor
- CED
- Differentiation inhibiting factor
see all -
機能
Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. -
組織特異性
Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage. -
関連疾患
Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision. -
配列類似性
Belongs to the TGF-beta family. -
翻訳後修飾
Glycosylated.
The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive. -
細胞内局在
Secreted > extracellular space > extracellular matrix. - Information by UniProt
画像
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All lanes : Anti-CTGF antibody [EPR20728] (ab209780) at 1/1000 dilution
Lane 1 : NIH/3T3 (mouse embryonic fibroblast) starved for 18 hours, whole cell lysate
Lane 2 : NIH/3T3 starved for 18 hours, then treated with 10 ng/ml transforming growth factor-ß (TGF-ß1, ab50036) and 50 µg/ml Heparin sodium salt for 24 hours, whole cell lysate
Lane 3 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilutionBlocking/Dilution buffer and concentration: 5% NFDM/TBST.
The level of CTGF expression can be induced by TGF beta treatment (PMID: 17786299).
CTGF is constitutively expressed in HepG2 cells (PMID:15886528).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (59)
ab50036 は 59 報の論文で使用されています。
- Li WJ et al. Cryptotanshinone Attenuated Pathological Cardiac Remodeling In Vivo and In Vitro Experiments. Oxid Med Cell Longev 2023:4015199 (2023). PubMed: 36743695
- Duran CL et al. Cooperative NF-κB and Notch1 signaling promotes macrophage-mediated MenaINV expression in breast cancer. Breast Cancer Res 25:37 (2023). PubMed: 37024946
- Yuan S et al. Integrating Network Pharmacology and Experimental Validation to Explore the Pharmacological Mechanism of Astragaloside IV in Treating Bleomycin-Induced Pulmonary Fibrosis. Drug Des Devel Ther 17:1289-1302 (2023). PubMed: 37138582
- Del Nero M et al. Advanced Cell Culture Models Illuminate the Interplay between Mammary Tumor Cells and Activated Fibroblasts. Cancers (Basel) 15:N/A (2023). PubMed: 37173963
- Kim HJ et al. A p-Tyr42 RhoA Inhibitor Promotes the Regeneration of Human Corneal Endothelial Cells by Ameliorating Cellular Senescence. Antioxidants (Basel) 12:N/A (2023). PubMed: 37371916