Recombinant human TGF alpha protein (Active) (ab283419)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% HPLC
- Endotoxin level: <= 0.005 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, HPLC, MS, Cell Culture, Biological Activity
製品の詳細
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製品名
Recombinant human TGF alpha protein (Active) -
生理活性
Fully biologically active determined by the dose dependent proliferationof Balb/c-3T3 cells. ED50 is ≤ 0.20 ng/ml, corresponding to a specific activity of 5 x 106 units/mg.
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精製度
>= 95 % HPLC. -
エンドトキシン・レベル
<=0.005 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
キャリア・フリー
はい -
由来
Recombinant -
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生物種
Human -
配列
ENSTSPLSADPPVAAAVVSHFNDCPDSHTQFCFHGTCRFLVQEDKPACVC HSGYVGARCEHADLLAVVAASQKKQ -
予測される分子量
17 kDa -
領域
24 to 98 -
配列の追加情報
N-terminal glycine.
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特性
Our Abpromise guarantee covers the use of ab283419 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
HPLC
Mass Spectrometry
Cell Culture
Biological Activity
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at Room Temperature. Store at Room Temperature.
pH: 7.4
Constituents: 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Monobasic dihydrogen potassium phosphate, 10.26% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Store lyophilized form at room temperature. Reconstitute in PBS, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
関連情報
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別名
- EGF like TGF
- EGF-like TGF
- ETGF
see all -
機能
TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar. -
組織特異性
Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines. -
配列類似性
Contains 1 EGF-like domain. -
細胞内局在
Cell membrane and Secreted > extracellular space. - Information by UniProt
画像
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Biological Activity - Recombinant human TGF alpha protein (Active) (ab283419)
Fully biologically active determined by the dose dependent proliferationof Balb/c-3T3 cells. ED50 is ≤ 0.20 ng/ml, corresponding to a specific activity of 5 x 106 units/mg.
Cell based assay testing is performed on the first lot of the protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.
Lot GR3429136-2
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SDS-PAGE - Recombinant human TGF alpha protein (ab283419)SDS-PAGE analysis of ab283419
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Mass Spectrometry - Recombinant human TGF alpha protein (ab283419)Mass determination by ESI-TOF.
Predicted MW is 8057 (+/- 10Da by ESI-TOF). MW observed is 8058.65.
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HPLC - Recombinant human TGF alpha protein (ab283419)HPLC analysis of ab283419
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab283419 は論文での使用が確認できていません。
