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AB63169

Recombinant human TAOK1 protein

Recombinant human TAOK1 protein

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Recombinant human TAOK1 protein is a Human Fragment protein, in the 1 to 314 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS.

別名を表示する

KIAA1361, MAP3K16, MARKK, TAOK1, Serine/threonine-protein kinase TAO1, Kinase from chicken homolog B, MARK Kinase, Prostate-derived sterile 20-like kinase 2, Thousand and one amino acid protein kinase 1, hKFC-B, PSK-2, PSK2, Prostate-derived STE20-like kinase 2, hTAOK1

4 Images
Functional Studies - Recombinant human TAOK1 protein (AB63169)
  • FuncS

Unknown

Functional Studies - Recombinant human TAOK1 protein (AB63169)

Sample Kinase Activity Plot.

Functional Studies - Recombinant human TAOK1 protein (AB63169)
  • FuncS

Unknown

Functional Studies - Recombinant human TAOK1 protein (AB63169)

The specific activity of TAOK1 (ab63169) was determined to be 28 nmol/min/mg as per activity assay protocol and was equivalent to 1630 nmol/min/mg as per radiometric assay

SDS-PAGE - Recombinant human TAOK1 protein (AB63169)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human TAOK1 protein (AB63169)

SDS PAGE analysis of ab63169

SDS-PAGE - Recombinant human TAOK1 protein (AB63169)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human TAOK1 protein (AB63169)

ab63169 on SDS-PAGE. Molecular Weight : ~63 kDa.

Key facts

精製度

>90% Densitometry

発現系

Baculovirus infected Sf9 cells

タグ

Tag free

アプリケーション

SDS-PAGE, FuncS

applications

生物活性

Yes

生物学的活性

Specific activity: 1670 nmol/min/mg.

アクセッション番号

Q7L7X3

アニマルフリー

No

キャリアフリー

No

種

Human

バッファー組成

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0038% EGTA, 0.00292% EDTA, 0.00174% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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製品の詳細

ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
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配列情報

[{"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":314,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q7L7X3","tags":[]}]
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出荷温度及び保存条件

出荷温度
Dry Ice
短期保存温度
-80°C
長期保存温度
-80°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
True
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

TAOK1 also known as TAO kinase 1 or PSK2 is a serine/threonine-protein kinase with a mass of about 125 kDa. It is expressed in various tissues including the brain heart and liver. This protein primarily influences cellular processes through phosphorylation which affects the activity of downstream effectors and can alter cellular responses to different stimuli. The full-length protein facilitates signal transduction pathways within cells leveraging its kinase activity to influence cellular structures and communication.
Biological function summary

TAOK1 participates in important cellular mechanisms such as cytoskeletal organization and cell cycle regulation. It functions as an integral component of complexes that manage these processes working through phosphorylation to modulate activities of other proteins. TAOK1 has a significant role in mitotic events ensuring proper chromosome segregation and maintaining genomic stability. Its kinase activity is important for signaling pathways that govern cellular responses to environmental challenges and stress.

Pathways

The participation of TAOK1 can especially be observed in the MAPK and stress-activated protein kinase (SAPK) pathways. These pathways are essential for transmitting extracellular signals into intracellular responses regulating processes like apoptosis and cytoskeletal rearrangement. TAOK1 interacts with related proteins such as MAP3K7 (also known as TAK1) to propagate signals that adapt cellular states under stress conditions. This interaction suggests a cooperative role in managing cellular response and survival during varying conditions.

TAOK1 exhibits associations with neurological disorders and cancer. Abnormal expression or mutation of TAOK1 affects its kinase activity contributing to conditions like neurodevelopmental disorders and glioblastoma. It interacts with key proteins such as p53 a well-known tumor suppressor gene underlying its maybe involvement in cancer pathways. These associations highlight the importance of TAOK1 as a potential biomarker or therapeutic target in disease management and treatment strategies.
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製品の性状

製品の状態

Liquid

補足情報

Affinity purified.

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一般的な情報

機能

Serine/threonine-protein kinase involved in various processes such as p38/MAPK14 stress-activated MAPK cascade, DNA damage response and regulation of cytoskeleton stability. Phosphorylates MAP2K3, MAP2K6 and MARK2. Acts as an activator of the p38/MAPK14 stress-activated MAPK cascade by mediating phosphorylation and subsequent activation of the upstream MAP2K3 and MAP2K6 kinases. Involved in G-protein coupled receptor signaling to p38/MAPK14. In response to DNA damage, involved in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 stress-activated MAPK cascade, probably by mediating phosphorylation of MAP2K3 and MAP2K6. Acts as a regulator of cytoskeleton stability by phosphorylating 'Thr-208' of MARK2, leading to activate MARK2 kinase activity and subsequent phosphorylation and detachment of MAPT/TAU from microtubules. Also acts as a regulator of apoptosis : regulates apoptotic morphological changes, including cell contraction, membrane blebbing and apoptotic bodies formation via activation of the MAPK8/JNK cascade. Plays an essential role in the regulation of neuronal development in the central nervous system (PubMed : 33565190). Also plays a role in the regulation of neuronal migration to the cortical plate (By similarity).

配列の類似性

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

翻訳後修飾

Proteolytically processed by caspase-3 (CASP3).. Autophosphorylated (By similarity). Phosphorylated by ATM in response to DNA damage. Phosphorylated by LRRK2.

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製品プロトコール

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ターゲットの情報

Serine/threonine-protein kinase involved in various processes such as p38/MAPK14 stress-activated MAPK cascade, DNA damage response and regulation of cytoskeleton stability. Phosphorylates MAP2K3, MAP2K6 and MARK2. Acts as an activator of the p38/MAPK14 stress-activated MAPK cascade by mediating phosphorylation and subsequent activation of the upstream MAP2K3 and MAP2K6 kinases. Involved in G-protein coupled receptor signaling to p38/MAPK14. In response to DNA damage, involved in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 stress-activated MAPK cascade, probably by mediating phosphorylation of MAP2K3 and MAP2K6. Acts as a regulator of cytoskeleton stability by phosphorylating 'Thr-208' of MARK2, leading to activate MARK2 kinase activity and subsequent phosphorylation and detachment of MAPT/TAU from microtubules. Also acts as a regulator of apoptosis : regulates apoptotic morphological changes, including cell contraction, membrane blebbing and apoptotic bodies formation via activation of the MAPK8/JNK cascade. Plays an essential role in the regulation of neuronal development in the central nervous system (PubMed : 33565190). Also plays a role in the regulation of neuronal migration to the cortical plate (By similarity).
See full target information TAOK1
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組合せ製品

Primary Antibodies

AB248030

Anti-TAOK2 + TAOK1 + TAOK3 (phospho S177 + S181 + S181) antibody [EPR4883] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-TAOK2 + TAOK1 + TAOK3 (phospho S177 + S181 + S181) antibody [EPR4883] - BSA and Azide free (AB248030)

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