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AB198639

Recombinant human Tankyrase protein (GST tag N-Terminus)

Recombinant human Tankyrase protein (GST tag N-Terminus)

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Recombinant human Tankyrase protein (GST tag N-Terminus) is a Human Fragment protein, in the 1001 to 1327 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS.

別名を表示する

PARP5A, PARPL, TIN1, TINF1, TNKS1, TNKS, Poly [ADP-ribose] polymerase tankyrase-1, ADP-ribosyltransferase diphtheria toxin-like 5, Poly [ADP-ribose] polymerase 5A, Protein poly-ADP-ribosyltransferase tankyrase-1, TNKS-1, TRF1-interacting ankyrin-related ADP-ribose polymerase, Tankyrase I, Tankyrase-1, ARTD5, TANK1

2 Images
Functional Studies - Recombinant human Tankyrase protein (GST tag N-Terminus) (AB198639)
  • FuncS

Supplier Data

Functional Studies - Recombinant human Tankyrase protein (GST tag N-Terminus) (AB198639)

Specific activity of ab198639.

SDS-PAGE - Recombinant human Tankyrase protein (GST tag N-Terminus) (AB198639)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human Tankyrase protein (GST tag N-Terminus) (AB198639)

4-20% SDS-PAGE with Coomassie staining

Lane 1 : 3.8 μg ab198639
Lane 2 : Protein marker

Key facts

精製度

>90% SDS-PAGE

発現系

Baculovirus infected Sf9 cells

タグ

GST tag N-Terminus

アプリケーション

SDS-PAGE, FuncS

applications

生物活性

Yes

生物学的活性

One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute from NAD into the acid-insoluble form.

アクセッション番号

O95271

アニマルフリー

No

キャリアフリー

No

種

Human

バッファー組成

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.48% Tris buffer, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.017% Potassium chloride

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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配列情報

[{"sequence":"ELAVGGASNAGDGAAGTERKEGEVAGLDMNISQFLKSLGLEHLRDIFETEQITLDVLADMGHEELKEIGINAYGHRHKLIKGVERLLGGQQGTNPYLTFHCVNQGTILLDLAPEDKEYQSVEEEMQSTIREHRDGGNAGGIFNRYNVIRIQKVVNKKLRERFCHRQKEVSEENHNHHNERMLFHGSPFINAIIHKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPTHKDRSCYICHRQMLFCRVTLGKSFLQFSTMKMAHAPPGHHSVIGRPSVNGLAYAEYVIYRGEQAYPEYLITYQIMKPEAPSQTATAAEQKT","proteinLength":"Fragment","predictedMolecularWeight":"62.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":1327,"aminoAcidStart":1001,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"O95271","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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出荷温度及び保存条件

出荷温度
Dry Ice
短期保存温度
-80°C
長期保存温度
-80°C
保管に関する情報
Avoid freeze / thaw cycle
True
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Tankyrase also known as TNKS and tankyrase-1 is an enzyme belonging to the poly(ADP-ribose) polymerase (PARP) family with a molecular mass of about 142 kDa. It is expressed in various tissues including brain heart and liver as well as in certain types of cancer cells. Mechanically tankyrase catalyzes the addition of ADP-ribose polymers to protein substrates a process known as poly(ADP-ribosyl)ation. This activity modulates the stability localization or interactions of target proteins.
Biological function summary

Tankyrase functions in diverse cellular processes. It participates in telomere maintenance by ADP-ribosylating TRF1 a telomere-binding protein leading to TRF1's disassembly from telomeres. Moreover tankyrase acts in the regulation of Wnt/β-catenin signaling where it associates with components like AXIN influencing the stability of the β-catenin destruction complex. Tankyrase can also be part of larger protein complexes such as the shelterin complex playing a role in safeguarding chromosome ends.

Pathways

Tankyrase integrates into key signaling networks. In the Wnt/β-catenin pathway tankyrase's interaction with AXIN controls the availability of β-catenin an important transcription co-activator in cell proliferation and differentiation. This enzyme also connects with the telomere maintenance pathway engaging TRF1 and contributing to telomere elongation and stability. Through these pathways tankyrase engages in processes vital for cell growth and genome integrity.

Tankyrase is implicated in cancer and heritable disorders of telomere dysfunction. Abnormal tankyrase activity correlates with increased β-catenin levels promoting oncogenesis in colorectal and other cancers. Additionally disruptions in tankyrase-mediated telomere regulation can lead to conditions like dyskeratosis congenita. Tankyrase links with proteins such as β-catenin in cancer while in telomere-related disorders it connects with TRF1 indicating its significance in maintaining cellular homeostasis.
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製品の性状

製品の状態

Liquid

補足情報

Affinity purified.

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一般的な情報

機能

Poly-ADP-ribosyltransferase involved in various processes such as Wnt signaling pathway, telomere length and vesicle trafficking (PubMed : 10988299, PubMed : 11739745, PubMed : 16076287, PubMed : 19759537, PubMed : 21478859, PubMed : 22864114, PubMed : 23622245, PubMed : 25043379, PubMed : 28619731). Acts as an activator of the Wnt signaling pathway by mediating poly-ADP-ribosylation (PARsylation) of AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex : poly-ADP-ribosylated target proteins are recognized by RNF146, which mediates their ubiquitination and subsequent degradation (PubMed : 19759537, PubMed : 21478859). Also mediates PARsylation of BLZF1 and CASC3, followed by recruitment of RNF146 and subsequent ubiquitination (PubMed : 21478859). Mediates PARsylation of TERF1, thereby contributing to the regulation of telomere length (PubMed : 11739745). Involved in centrosome maturation during prometaphase by mediating PARsylation of HEPACAM2/MIKI (PubMed : 22864114). May also regulate vesicle trafficking and modulate the subcellular distribution of SLC2A4/GLUT4-vesicles (PubMed : 10988299). May be involved in spindle pole assembly through PARsylation of NUMA1 (PubMed : 16076287). Stimulates 26S proteasome activity (PubMed : 23622245).

配列の類似性

Belongs to the ARTD/PARP family.

翻訳後修飾

Phosphorylated on serine residues by MAPK kinases upon insulin stimulation. Phosphorylated during mitosis.. Ubiquitinated by RNF146 when auto-poly-ADP-ribosylated, leading to its degradation. Deubiquitinated by USP25; leading to stabilization (PubMed:28619731).. ADP-ribosylated (-auto). Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination.

細胞内局在性

Cytoskeleton

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製品プロトコール

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ターゲットの情報

Poly-ADP-ribosyltransferase involved in various processes such as Wnt signaling pathway, telomere length and vesicle trafficking (PubMed : 10988299, PubMed : 11739745, PubMed : 16076287, PubMed : 19759537, PubMed : 21478859, PubMed : 22864114, PubMed : 23622245, PubMed : 25043379, PubMed : 28619731). Acts as an activator of the Wnt signaling pathway by mediating poly-ADP-ribosylation (PARsylation) of AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex : poly-ADP-ribosylated target proteins are recognized by RNF146, which mediates their ubiquitination and subsequent degradation (PubMed : 19759537, PubMed : 21478859). Also mediates PARsylation of BLZF1 and CASC3, followed by recruitment of RNF146 and subsequent ubiquitination (PubMed : 21478859). Mediates PARsylation of TERF1, thereby contributing to the regulation of telomere length (PubMed : 11739745). Involved in centrosome maturation during prometaphase by mediating PARsylation of HEPACAM2/MIKI (PubMed : 22864114). May also regulate vesicle trafficking and modulate the subcellular distribution of SLC2A4/GLUT4-vesicles (PubMed : 10988299). May be involved in spindle pole assembly through PARsylation of NUMA1 (PubMed : 16076287). Stimulates 26S proteasome activity (PubMed : 23622245).
See full target information TNKS
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