AB198755

Recombinant human Tankyrase 2/TNKS2 protein (GST tag N-Terminus)

Name: Recombinant human Tankyrase 2/TNKS2 protein (ab198755) | Abcam
Brand: Abcam Limited
SKU: AB198755
Price: 325000 JPY
Availability: InStock

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Recombinant human Tankyrase 2/TNKS2 protein (GST tag N-Terminus) is a Human Fragment protein, in the 667 to 1166 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS.

別名を表示する

PARP5B, TANK2, TNKL, TNKS2, Poly [ADP-ribose] polymerase tankyrase-2, ADP-ribosyltransferase diphtheria toxin-like 6, Poly [ADP-ribose] polymerase 5B, Protein poly-ADP-ribosyltransferase tankyrase-2, TNKS-2, TRF1-interacting ankyrin-related ADP-ribose polymerase 2, Tankyrase II, Tankyrase-2, Tankyrase-like protein, Tankyrase-related protein, ARTD6

2 Images

Functional Studies - Recombinant human Tankyrase 2/TNKS2 protein (GST tag N-Terminus) (AB198755)

FuncS

Supplier Data

Functional Studies - Recombinant human Tankyrase 2/TNKS2 protein (GST tag N-Terminus) (AB198755)

Activity assay using ab198755.

SDS-PAGE - Recombinant human Tankyrase 2/TNKS2 protein (GST tag N-Terminus) (AB198755)

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human Tankyrase 2/TNKS2 protein (GST tag N-Terminus) (AB198755)

4-20% SDS-PAGE analysis of 2.5 μg ab198755 with Coomassie staining.

Key facts

精製度

>90% SDS-PAGE

発現系

Baculovirus infected Sf9 cells

アプリケーション

FuncS, SDS-PAGE

applications

生物活性

Yes

生物学的活性

Specific Activity: ≥16259 U/μg.

Unit Definition: One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute from NAD into the acid-insoluble form.

Assay Conditions: Enzyme reaction is conducted using a Tankyrase 2/TNKS2 Histone Ribosylation Assay Kit (Biotin-labeled NAD+).

アクセッション番号

Q9H2K2

アニマルフリー

キャリアフリー

種

Human

バッファー組成

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Previously labelled as Tankyrase 2.

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配列情報

[{"sequence":"PDNVNCRDTQGRHSTPLHLAAGYNNLEVAEYLLQHGADVNAQDKGGLIPLHNAASYGHVDVAALLIKYNACVNATDKWAFTPLHEAAQKGRTQLCALLLAHGADPTLKNQEGQTPLDLVSADDVSALLTAAMPPSALPSCYKPQVLNGVRSPGATADALSSGPSSPSSLSAASSLDNLSGSFSELSSVVSSSGTEGASSLEKKEVPGVDFSITQFVRNLGLEHLMDIFEREQITLDVLVEMGHKELKEIGINAYGHRHKLIKGVERLISGQQGLNPYLTLNTSGSGTILIDLSPDDKEFQSVEEEMQSTVREHRDGGHAGGIFNRYNILKIQKVCNKKLWERYTHRRKEVSEENHNHANERMLFHGSPFVNAIIHKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPVHKDRSCYICHRQLLFCRVTLGKSFLQFSAMKMAHSPPGHHSVTGRPSVNGLALAEYVIYRGEQAYPEYLITYQIMRPEGMVDG","proteinLength":"Fragment","predictedMolecularWeight":"80.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":1166,"aminoAcidStart":667,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q9H2K2","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]

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出荷温度及び保存条件

出荷温度

Dry Ice

短期保存温度

-80°C

長期保存温度

-80°C

保管に関する情報

Avoid freeze / thaw cycle

True

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Tankyrase 2 also known as TNKS2 functions as a poly(ADP-ribose) polymerase adding ADP-ribose polymers to target proteins. It has a molecular weight of approximately 135 kDa. Expression of TNKS2 occurs in various tissues including the brain heart and skeletal muscle. This protein consists of several domains such as an ankyrin repeat domain and a sterile alpha motif (SAM) domain allowing it to interact with different substrates and mediate protein-protein interactions.

Biological function summary

TNKS2 plays a significant role in telomere maintenance and Wnt signaling. It is a component of the telomere-associated complex which helps regulate telomere length and stability by interacting with TRF1 a telomere-binding factor. In the context of Wnt signaling it functions by modulating the stability of Axin a scaffold protein thereby affecting the degradation of β-catenin. By ensuring proper telomere function and Wnt signaling TNKS2 supports cellular processes such as proliferation and differentiation.

Pathways

TNKS2 contributes notably to telomere maintenance and Wnt/β-catenin signaling pathways. In telomere maintenance its activity influences telomerase access to telomeres cooperating with proteins like TRF1 and TIN2. Within the Wnt/β-catenin signaling pathway TNKS2 interacts with Axin promoting β-catenin accumulation which in turn stimulates gene transcription necessary for cell growth and development. These two pathways demonstrate how TNKS2 is involved in regulating fundamental aspects of cell biology and growth.

TNKS2 associates with conditions such as cancer and fibrosis. In various cancers aberrant TNKS2 activity results in dysregulation of Wnt signaling leading to improper cell proliferation and tumor growth. For example its interaction with β-catenin contributes to oncogenesis in colorectal cancer. In fibrosis altered TNKS2 function disrupts normal signaling processes which can lead to the excessive connective tissue accumulation characteristic of fibrotic diseases. Therefore TNKS2 appears as a promising target in therapeutic strategies aiming to modulate these pathways and control disease progression.

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製品の性状

製品の状態

Liquid

補足情報

Affinity purified.

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一般的な情報

機能

Poly-ADP-ribosyltransferase involved in various processes such as Wnt signaling pathway, telomere length and vesicle trafficking (PubMed : 11739745, PubMed : 11802774, PubMed : 19759537, PubMed : 21478859, PubMed : 23622245, PubMed : 25043379). Acts as an activator of the Wnt signaling pathway by mediating poly-ADP-ribosylation of AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex : poly-ADP-ribosylated target proteins are recognized by RNF146, which mediates their ubiquitination and subsequent degradation (PubMed : 19759537, PubMed : 21478859). Also mediates poly-ADP-ribosylation of BLZF1 and CASC3, followed by recruitment of RNF146 and subsequent ubiquitination (PubMed : 21478859). Mediates poly-ADP-ribosylation of TERF1, thereby contributing to the regulation of telomere length (PubMed : 11739745). Stimulates 26S proteasome activity (PubMed : 23622245).

配列の類似性

Belongs to the ARTD/PARP family.

翻訳後修飾

Ubiquitinated at 'Lys-48' and 'Lys-63' by RNF146 when auto-poly-ADP-ribosylated; this leads to degradation. Deubiquitinated by USP25; leading to stabilization (By similarity).. ADP-ribosylated (-auto). Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination.. The crystallographic evidence suggests that the 3-hydroxyhistidine may be the (3S) stereoisomer.

細胞内局在性

Nucleus

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information TNKS2

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Recombinant human Tankyrase 2/TNKS2 protein (GST tag N-Terminus)