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AB131680

Recombinant Human Syntaxin 4 protein (His tag N-Terminus)

Recombinant Human Syntaxin 4 protein (His tag N-Terminus)

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Recombinant Human Syntaxin 4 protein (His tag N-Terminus) is a Human Fragment protein, in the 1 to 275 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

別名を表示する

STX4A, STX4, Syntaxin-4, Renal carcinoma antigen NY-REN-31

1 Images
SDS-PAGE - Recombinant Human Syntaxin 4 protein (His tag N-Terminus) (AB131680)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Syntaxin 4 protein (His tag N-Terminus) (AB131680)

15% SDS-PAGE analysis of 3 µg ab131680.

Key facts

精製度

>90% SDS-PAGE

発現系

Escherichia coli

タグ

His tag N-Terminus

アプリケーション

Mass Spec, SDS-PAGE

applications

生物活性

No

アクセッション番号

Q12846

アニマルフリー

No

キャリアフリー

No

種

Human

バッファー組成

pH: 8 Constituents: 40% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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配列情報

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSHMMRDRTHELRQGDDSSDEEDKERVALVVHPGTARLGSPDEEFFHKVRTIRQTIVKLGNKVQELEKQQVTILATPLPEESMKQELQNLRDEIKQLGREIRLQLKAIEPQKEEADENYNSVNTRMRKTQHGVLSQQFVELINKCNSMQSEYREKNVERIRRQLKITNAGMVSDEELEQMLDSGQSEVFVSNILKDTQVTRQALNEISARHSEIQQLERSIRELHDIFTFLATEVEMQGEMINRIEKNILSSADYVERGQEHVKTALENQKKARKKKVL","proteinLength":"Fragment","predictedMolecularWeight":"34.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":275,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q12846","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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出荷温度及び保存条件

出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
False
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Syntaxin 4 also known as STX4 is a member of the soluble NSF attachment protein receptor (SNARE) family. It has a molecular mass of approximately 33 kDa. Syntaxin 4 is integral to membrane fusion processes specifically docking and fusion of vesicles with target membranes. It expresses in a wide range of tissues but high levels are found in skeletal muscle and adipose tissue. Its function is necessary in cellular trafficking systems and exocytosis.
Biological function summary

Syntaxin 4 facilitates key transport events at the plasma membrane. It forms part of the SNARE complex which is essential for vesicle targeting and fusion. This complex includes other SNARE proteins such as VAMP2 and SNAP23 ensuring that vesicles deliver their content accurately within cells. This function is critical in processes such as insulin-stimulated glucose uptake and neurotransmitter release.

Pathways

Syntaxin 4 involves itself in the regulation of vesicular transport and insulin signaling. It plays an essential role in the GLUT4 translocation pathway where Syntaxin 4 interacts with proteins like Munc18c which is critical for the regulation of glucose transport in response to insulin. Another important pathway includes its involvement in neurotransmitter release where Syntaxin 4 interfaces with synaptotagmin highlighting its influence within signal transmission processes.

Dysfunctions related to Syntaxin 4 have implications in conditions such as type 2 diabetes and certain neuropathies. In the context of type 2 diabetes impaired Syntaxin 4-mediated uptake of glucose into cells can lead to insulin resistance. Syntaxin 4 also relates to neuromuscular disorders where its malfunction can alter neurotransmitter release. These conditions oftentimes involve Munc18c which stabilizes Syntaxin 4 and modulates its activity within the cell.
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製品の性状

製品の状態

Liquid

補足情報

ab131680 was purified using conventional chromatography techniques.

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一般的な情報

機能

Plasma membrane t-SNARE that mediates docking of transport vesicles (By similarity). Necessary for the translocation of SLC2A4 from intracellular vesicles to the plasma membrane (By similarity). In neurons, recruited at neurite tips to membrane domains rich in the phospholipid 1-oleoyl-2-palmitoyl-PC (OPPC) which promotes neurite tip surface expression of the dopamine transporter SLC6A3/DAT by facilitating fusion of SLC6A3-containing transport vesicles with the plasma membrane (By similarity). Together with STXB3 and VAMP2, may also play a role in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes and in docking of synaptic vesicles at presynaptic active zones (By similarity). Required for normal hearing (PubMed : 36355422).

配列の類似性

Belongs to the syntaxin family.

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製品プロトコール

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ターゲットの情報

Plasma membrane t-SNARE that mediates docking of transport vesicles (By similarity). Necessary for the translocation of SLC2A4 from intracellular vesicles to the plasma membrane (By similarity). In neurons, recruited at neurite tips to membrane domains rich in the phospholipid 1-oleoyl-2-palmitoyl-PC (OPPC) which promotes neurite tip surface expression of the dopamine transporter SLC6A3/DAT by facilitating fusion of SLC6A3-containing transport vesicles with the plasma membrane (By similarity). Together with STXB3 and VAMP2, may also play a role in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes and in docking of synaptic vesicles at presynaptic active zones (By similarity). Required for normal hearing (PubMed : 36355422).
See full target information STX4
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組合せ製品

Primary Antibodies

AB184545

Anti-Syntaxin 4 antibody [EPR15473]

RabMAb|Recombinant|KO Validated

Western blot - Anti-Syntaxin 4 antibody [EPR15473] (AB184545)

  • 0
  • 0
Primary Antibodies

AB88056

Anti-Glucokinase antibody

Immunocytochemistry/ Immunofluorescence - Anti-Glucokinase antibody (AB88056)

  • 5
  • 1
Primary Antibodies

AB133673

Anti-Alpha SNAP antibody [EPR7372]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha SNAP antibody [EPR7372] (AB133673)

  • 0
  • 0
Primary Antibodies

AB272736

Anti-Syntaxin 1a antibody [EPR23457-15]

BOND RX™ Validated|RabMAb|Recombinant

Immunoprecipitation - Anti-Syntaxin 1a antibody [EPR23457-15] (AB272736)

  • 0
  • 0

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