Recombinant human SynCAM/CADM1 protein (ab167740)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE, ELISA
製品の詳細
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製品名
Recombinant human SynCAM/CADM1 protein
SynCAM/CADM1 タンパク質・ペプチド 製品一覧 -
生理活性
Measured by its binding ability in a functional ELISA. Immobilized rhCADM1 at 2 µg/ml (100 µl/well) can bind biotinylated Human CRTAM with a linear range of 12.5 – 500 ng/ml. -
精製度
> 95 % SDS-PAGE.
ab167740 was lyophilized from 0.22 µm filtered solution. -
エンドトキシン・レベル
< 1.000 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
QNLFTKDVTVIEGEVATISCQVNKSDDSVIQLLNPNRQTIYFRDFRPLKD SRFQLLNFSSSELKVSLTNVSISDEGRYFCQLYTDPPQESYTTITVLVPP RNLMIDIQKDTAVEGEEIEVNCTAMASKPATTIRWFKGNTELKGKSEVEE WSDMYTVTSQLMLKVHKEDDGVPVICQVEHPAVTGNLQTQRYLEVQYKPQ VHIQMTYPLQGLTREGDALELTCEAIGKPQPVMVTWVRVDDEMPQHAVLS GPNLFINNLNKTDNGTYRCEASNIVGKAHSDYMLYVYDPPTTIPPPTTTT TTTTTTTTTILTIITDSRAGEEGSIRAVDH -
予測される分子量
39 kDa including tags -
領域
45 to 374 -
タグ
His tag C-Terminus
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関連製品
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Positive Controls
特性
Our Abpromise guarantee covers the use of ab167740 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
ELISA
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製品の状態
Lyophilized -
備考
This product was previously labelled as SynCAM
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 94% PBS, 5% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成It is recommended to reconstitute the lyophilized protein in sterile deionized water to a final concentration of 100 ug/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.
関連情報
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別名
- BL2
- Cadm1
- CADM1_HUMAN
see all -
機能
Mediates homophilic cell-cell adhesion in a Ca(2+)-independent manner. Also mediates heterophilic cell-cell adhesion with CADM3 and PVRL3 in a Ca(2+)-independent manner. Acts as a tumor suppressor in non-small-cell lung cancer (NSCLC) cells. Interaction with CRTAM promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM3 in vivo. May contribute to the less invasive phenotypes of lepidic growth tumor cells. In mast cells, may mediate attachment to and promote communication with nerves. CADM1, together with MITF, is essential for development and survival of mast cells in vivo. May act as a synaptic cell adhesion molecule that drives synapse assembly. May be involved in neuronal migration, axon growth, pathfinding, and fasciculation on the axons of differentiating neurons. May play diverse roles in the spermatogenesis including in the adhesion of spermatocytes and spermatids to Sertoli cells and for their normal differentiation into mature spermatozoa. -
配列類似性
Belongs to the nectin family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
ドメイン
The cytoplasmic domain appears to play a critical role in proapoptosis and tumor suppressor activity in NSCLC. -
細胞内局在
Cell membrane. Associates with perinuclear and plasma membranes in vivo. Localized to the basolateral plasma membrane of epithelial cells in gall bladder. - Information by UniProt
画像
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SDS-PAGE - Recombinant human SynCAM/CADM1 protein (ab167740)Human CADM1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
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Functional Studies - Recombinant human SynCAM/CADM1 protein (ab167740)Immobilized Cynomolgus CRTAM, Fc Tag at 5 µg/mL (100 µL/well) can bind Human CADM1, His Tag with a linear range of 0.6-5 ng/mL. -
SDS-PAGE - Recombinant human SynCAM/CADM1 protein (ab167740)SDS-PAGE analysis of reduced ab167740 and staining overnight with Coomassie Blue. In DTT-reduced SDS-PAGE, SynCAM/CADM1 protein migrates as 38.5kDa, 50kDa and 55-85 kDa poly peptide due to different glycosylation.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab167740 は論文での使用が確認できていません。
