Recombinant Human Survivin protein (ab87202)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: Calmodulin tag N-Terminus
- Suitable for: SDS-PAGE, Sandwich ELISA
製品の詳細
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製品名
Recombinant Human Survivin protein
Survivin タンパク質・ペプチド 製品一覧 -
精製度
> 90 % SDS-PAGE.
ab87202 is purified using conventional chromatography techniques. -
発現系
Escherichia coli -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
MGAPTLPPAWQPFLKDHRISTFKNWPFLEGCACTPERMAEAGFIHCPTEN EPDLAQCFFCFKELEGWEPDDDPIEEHKKHSSGCAFLSVKKQFEELTLGE FLKLDRERAKNKIAKETNNKKKEFEETAKKVRRAIEQLAAMD -
予測される分子量
33 kDa -
領域
1 to 142 -
タグ
Calmodulin tag N-Terminus
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab87202 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Sandwich ELISA
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.242% Tris, 0.58% Sodium chloride
関連情報
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別名
- API4
- Apoptosis inhibitor 4
- Apoptosis inhibitor survivin
see all -
機能
Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May play a role in neoplasia. May counteract a default induction of apoptosis in G2/M phase. Inhibitor of caspase-3 and caspase-7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform. -
組織特異性
Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines. -
配列類似性
Belongs to the IAP family.
Contains 1 BIR repeat. -
発生段階
Expression is cell cycle-dependent and peaks at mitosis. -
ドメイン
The BIR repeat is necessary and sufficient for HBXIP binding. -
翻訳後修飾
Ubiquitination is required for centrosomal targeting.
In vitro phosphorylation at Thr-117 by AURKB/STK12 prevents interaction with INCENP and localization to mitotic chromosomes. -
細胞内局在
Cytoplasm. Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with AURKB at mitotic chromosomes. - Information by UniProt
画像
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Sandwich ELISA - Recombinant Human Survivin protein (ab87202)
Standard curve for Survivin (Analyte: ab87202); dilution range 1pg/ml to 1µg/ml using Capture Antibody ab27468 at 1µg/ml and Detector Antibody Rabbit monoclonal [EP2880Y] to Survivin (ab76424) at 0.5µg/ml. Concentration of Rabbit monoclonal [EP2880Y] to Survivin (ab76424) may vary from lot to lot; please use this curve as guideline.
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SDS-PAGE - Recombinant Human Survivin protein (ab87202)15% SDS-PAGE showing ab87202 at approximately 33kDa (3µg).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (3)
ab87202 は 3 報の論文で使用されています。
- Liu J et al. Probing low-copy-number proteins in single living cells using single-cell plasmonic immunosandwich assays. Nat Protoc 16:3522-3546 (2021). PubMed: 34089021
- Galbo PM et al. Circulating CD9+/GFAP+/survivin+ exosomes in malignant glioma patients following survivin vaccination. Oncotarget 8:114722-114735 (2017). PubMed: 29383115
- Zeh HJ et al. First-in-man study of western reserve strain oncolytic vaccinia virus: safety, systemic spread, and antitumor activity. Mol Ther 23:202-14 (2015). PubMed: 25292189
