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AB74916

Recombinant human Superoxide Dismutase 1 protein (Tag Free)

Recombinant human Superoxide Dismutase 1 protein (Tag Free)

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Recombinant human Superoxide Dismutase 1 protein (Tag Free) is a Human Full Length protein, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, FuncS.

別名を表示する

Superoxide dismutase [Cu-Zn], Hydrogen sulfide oxidase, Superoxide dismutase 1, hSod1, SOD1

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SDS-PAGE - Recombinant human Superoxide Dismutase 1 protein (Tag Free) (AB74916)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human Superoxide Dismutase 1 protein (Tag Free) (AB74916)

15% SDS-PAGE of ab74916 (3μg).

Key facts

精製度

>95% SDS-PAGE

ab74916 is purified by conventional chromatography techniques.

発現系

Escherichia coli

タグ

Tag free

アプリケーション

FuncS, SDS-PAGE

applications

生物活性

Yes

生物学的活性

Specific activity is > 500unit/mg, in which one unit will inhibit the rate of reduction of cytochrome c by 50% in a coupled system, using xanthine and Xanthine oxidase at pH 7.5 at 25°C.

Activity Assay

  1. Prepare a 1.5 ml reaction mix into a suitable container and pre-chill on ice before use: The final concentrations are 50mM potassium phosphate, 0.1mM ethylendiaminetetraacetic acid, 0.01mM cytochrome C 0.05mM xanthine, 0.005 units xanthine oxidase.
  2. Equilibrate to 25°C and monitor at A550nm until the value is constant using a spectrophotometer.
  3. Add 50 ul of recombinant SOD protein in various concentrations (0.5ug, 1ug) in assay buffer.
  4. Mix by inversion and record the increase at A550nm for 5 minutes.
アクセッション番号

P00441

アニマルフリー

No

キャリアフリー

No

種

Human

バッファー組成

pH: 7.5 Constituents: 10% Glycerol (glycerin, glycerine), 0.242% Tris

storage-buffer

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組合せ製品

Reagents, Controls & Accessories

AB325994

Prestained Protein Ladder - Mid-range molecular weight (11-180 kDa, 4-color)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (11-180 kDa, 4-color) (AB325994)

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Assay Kits

AB65354

Superoxide Dismutase Activity Assay Kit (Colorimetric)

Functional Studies - Superoxide Dismutase Activity Assay Kit (Colorimetric) (AB65354)

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Proteins & Peptides

AB89359

Recombinant Human Carbonic Anhydrase 1/CA1 protein (His tag N-Terminus)

Western blot - Recombinant Human Carbonic Anhydrase 1/CA1 protein (His tag N-Terminus) (AB89359)

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Proteins & Peptides

AB139234

Recombinant Human MST protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human MST protein (His tag N-Terminus) (AB139234)

  • 0
  • 0
Proteins & Peptides

AB153778

Recombinant Human Cysteine Dioxygenase CDO1 protein

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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配列情報

[{"linker":null,"sequence":"MATKAVCVLKGDGPVQGIINFEQKESNGPVKVWGSIKGLTEGLHGFHVHEFGDNTAGCTSAGPHFNPLSRKHGGPKDEERHVGDLGNVTADKDGVADVSIEDSVISLSGDHCIIGRTLVVHEKADDLGKGGNEESTKTGNAGSRLACGVIGIAQ","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P00441","tags":[]}]
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出荷温度及び保存条件

製品の状態
Liquid
出荷温度
Blue Ice
短期保存温度
-20°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
True
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Superoxide Dismutase 1 (SOD1) also known as Cu/Zn SOD or simply dismutase is an enzyme important for the detoxification of superoxide radicals. SOD1 catalyzes the conversion of two superoxide molecules into oxygen and hydrogen peroxide maintaining cellular redox balance. This enzyme typically exhibits a mass of approximately 32 kDa. SOD1 is expressed in the cytoplasm of cells throughout the body including tissues like liver kidney and brain.
Biological function summary

The enzyme functions as a homodimer with each subunit containing a copper and zinc ion. These metal ions are essential for the catalytic activity of SOD1 as the copper ion participates in electron transfer while the zinc ion provides structural stability. The enzyme protects cells from oxidative stress by neutralizing excess reactive oxygen species ensuring cellular health and functioning.

Pathways

SOD1 plays an important role in the cellular antioxidant defense system and is a part of the reactive oxygen species (ROS) metabolic pathway. It works in conjunction with catalase and glutathione peroxidase to limit oxidative damage within cells. The close interaction between these enzymes highlights the interdependence within the antioxidant defense network emphasizing their role in maintaining cellular homeostasis.

Mutations in the SOD1 gene are linked to familial Amyotrophic Lateral Sclerosis (ALS) a neurodegenerative condition. In ALS aberrant SOD1 proteins may lead to increased oxidative stress and motor neuron damage. Research also connects SOD1 to the pathogenesis of Alzheimer's disease where oxidative stress is a contributing factor. These associations underline SOD1's significance in neurodegenerative diseases highlighting potential therapeutic targets for interventions focused on oxidative stress management.
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一般的な情報

機能

Destroys radicals which are normally produced within the cells and which are toxic to biological systems (PubMed : 24140062). Catalyzes the oxidation of hydrogen sulfide (H2S) to sulfate, playing an important role in detoxifying H2S and limiting the accumulation of reactive sulfur species (RSS) such as persulfides and polysulfides (PubMed : 36630448).

配列の類似性

Belongs to the Cu-Zn superoxide dismutase family.

翻訳後修飾

Unlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation.. The ditryptophan cross-link at Trp-33 is responsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required.. Palmitoylation helps nuclear targeting and decreases catalytic activity.. Succinylation, adjacent to copper catalytic site, probably inhibits activity. Desuccinylation by SIRT5 enhances activity.

細胞内局在性

Nucleus

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製品プロトコール

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ターゲットの情報

Destroys radicals which are normally produced within the cells and which are toxic to biological systems (PubMed : 24140062). Catalyzes the oxidation of hydrogen sulfide (H2S) to sulfate, playing an important role in detoxifying H2S and limiting the accumulation of reactive sulfur species (RSS) such as persulfides and polysulfides (PubMed : 36630448).
See full target information SOD1
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