Recombinant Human STUB1/CHIP protein (ab82791)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Suitable for: SDS-PAGE, WB
製品の詳細
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製品名
Recombinant Human STUB1/CHIP protein
STUB1/CHIP タンパク質・ペプチド 製品一覧 -
精製度
> 90 % SDS-PAGE. -
発現系
Escherichia coli -
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
MKGKEEKEGG ARLGAGGGSP EKSPSAQELK EQGNRLFVGR KYPEAAACYG RAITRNPLVA VYYTNRALCY LKMQQHEQAL ADCRRALELD GQSVKAHFFL GQCQLEMESY DEAIANLQRA YSLAKEQRLN FGDDIPSALR IAKKKRWNSI EERRIHQESE LHSYLSRLIA AERERELEEC QRNHEGDEDD SHVRAQQACI EAKHDKYMAD MDELFSQVDE KRKKRDIPDY LCGKISFELM REPCITPSGI TYDRKDIEEH LQRVGHFDPV TRSPLTQEQL IPNLAMKEVI DAFISENGWV EDY -
領域
1 to 303
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab82791 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Western blot
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製品の状態
Liquid -
備考
Previously labelled as STUB1.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.50
Constituents: 0.077% DTT, 0.316% Tris HCl, 10% Glycerol (glycerin, glycerine)
関連情報
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別名
- Antigen NY CO 7
- Antigen NY-CO-7
- C terminus of Hsp70-interacting protein
see all -
機能
E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. Ubiquitinates NOS1 in concert with Hsp70 and Hsp40. Modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. Mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. Mediates polyubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair: catalyzes polyubiquitination by amplifying the HUWE1/ARF-BP1-dependent monoubiquitination and leading to POLB-degradation by the proteasome. Mediates polyubiquitination of CYP3A4. -
組織特異性
Highly expressed in skeletal muscle, heart, pancreas, brain and placenta. Detected in kidney, liver and lung. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Contains 3 TPR repeats.
Contains 1 U-box domain. -
ドメイン
The TPR domain is essential for ubiquitination mediated by UBE2D1. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR.
Auto-ubiquitinated; mediated by UBE2D1 and UBE2D2. -
細胞内局在
Cytoplasm. - Information by UniProt
画像
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SDS-PAGE - Recombinant Human STUB1/CHIP protein (ab82791)15% SDS-PAGE showing ab82791 at approximately 34.8kDa (3µg).
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Western blot - Recombinant Human STUB1/CHIP protein (ab82791)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab82791 は 1 報の論文で使用されています。
- Labrador-Garrido A et al. Chaperome screening leads to identification of Grp94/Gp96 and FKBP4/52 as modulators of the a-synuclein-elicited immune response. FASEB J 30:564-77 (2016). PubMed: 26443817