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Recombinant Human STAT5a protein (ab84627)

Submit a review Q&A (1)References (1)
Western blot - Recombinant Human STAT5a protein (ab84627)
  • SDS-PAGE - Recombinant Human STAT5a protein (ab84627)

Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Purity: > 90% SDS-PAGE
  • Tags: His tag N-Terminus
  • Suitable for: WB, SDS-PAGE

製品の詳細

  • 製品名

    Recombinant Human STAT5a protein

  • 精製度

    > 90 % SDS-PAGE.
    Purity >90% as determined by densitometry. Affinity purified.

  • 発現系

    Baculovirus infected Sf9 cells

  • タンパク質長

    Full length protein

  • Animal free

    No

  • 由来

    Recombinant

    • 生物種

      Human

    • タグ

      His tag N-Terminus

特性

Our Abpromise guarantee covers the use of ab84627 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Western blot

    SDS-PAGE

  • 製品の状態

    Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.00
    Preservative: 1.02% Imidazole
    Constituents: 0.00174% PMSF, 0.82% Sodium phosphate, 0.00308% DTT, 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride

関連情報

  • 別名

    • Mammary gland factor
    • MGF
    • Signal transducer and activator of transcription 5A
    • STA5A_HUMAN
    • STAT 5
    • STAT 5A
    • STAT5
    • STAT5A
    see all

  • 機能

    Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.

  • 配列類似性

    Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.

  • 翻訳後修飾

    Tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity.

  • 細胞内局在

    Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
  • Information by UniProt

画像

  • Western blot - Recombinant Human STAT5a protein (ab84627)
    Western blot - Recombinant Human STAT5a protein (ab84627)
    Anti-STAT5a antibody [E289] (ab32043) at 1/1000 dilution (unpurified) + Recombinant Human STAT5a protein (ab84627) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 92 kDa


    Exposure time: 20 seconds
  • SDS-PAGE - Recombinant Human STAT5a protein (ab84627)
    SDS-PAGE - Recombinant Human STAT5a protein (ab84627)
    SDS Page analysis of ab84627

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (1)

ab84627 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab84627 は 1 報の論文で使用されています。

  • Zeng Y  et al. Inhibition of STAT5a by Naa10p contributes to decreased breast cancer metastasis. Carcinogenesis 35:2244-53 (2014). PubMed: 24925029

レビューと Q&A

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レビューを送る 質問を送る

Question

Does the protein have biological activity and could it be used in that way?

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Answer

Thank you for contacting us.

The lab sent me the following information:

The protein was expressed using the SF9 cells which has post-translational modification capabilities. Although we have not conducted any activity assays for the STAT protein, it highly likely that there is some basal activity for the protein.

If you like to try the protein that way, we have a discount program you can use in that regard.
For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:

The testing discount program is for customers who like to use an antibody/protein/kit on an untested species/application. You would purchase the antibody/protein/kit at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody/protein at the full price (100%) of the antibody/protein you have tested, or a full price discount for the amount paid for the kit. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.
This programapplies to ELISA kits and other kits we offer.

Please let me know if you would be interested in receiving a discount code.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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