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AB190389

Recombinant ヒト Sps1/Ste20 related kinase 4 protein (GST tag N-Terminus)

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Recombinant human Sps1/Ste20 related kinase 4 protein (GST tag N-Terminus) is a Human Fragment protein, in the 941 to 1328 aa range, expressed in Baculovirus infected Sf9 cells, with >80%, suitable for SDS-PAGE, WB, FuncS.

別名を表示する

RCK, YSK4, MAP3K19, Mitogen-activated protein kinase kinase kinase 19, SPS1/STE20-related protein kinase YSK4

4 Images
Functional Studies - Recombinant human Sps1/Ste20 related kinase 4 protein (GST tag N-Terminus) (AB190389)
  • FuncS

Supplier Data

Functional Studies - Recombinant human Sps1/Ste20 related kinase 4 protein (GST tag N-Terminus) (AB190389)

Kinase assay showing the specific activity of ab190389 as 38 nmol/min/mg.

Functional Studies - Recombinant human Sps1/Ste20 related kinase 4 protein (GST tag N-Terminus) (AB190389)
  • FuncS

Supplier Data

Functional Studies - Recombinant human Sps1/Ste20 related kinase 4 protein (GST tag N-Terminus) (AB190389)

The specific activity of Sps1/Ste20 related kinase 4 (ab190389) was determined to be 44 nmol/min/mg as per activity assay protocol.

SDS-PAGE - Recombinant human Sps1/Ste20 related kinase 4 protein (GST tag N-Terminus) (AB190389)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human Sps1/Ste20 related kinase 4 protein (GST tag N-Terminus) (AB190389)

SDS-PAGE analysis of ab190389.

SDS-PAGE - Recombinant human Sps1/Ste20 related kinase 4 protein (GST tag N-Terminus) (AB190389)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human Sps1/Ste20 related kinase 4 protein (GST tag N-Terminus) (AB190389)

SDS PAGE analysis of ab190389

Key facts

精製度

>80% Densitometry

Affinity purified.

発現系

Baculovirus infected Sf9 cells

タグ

GST tag N-Terminus

アプリケーション

SDS-PAGE, FuncS, WB

applications

生物活性

Yes

生物学的活性

The specific activity of ab190389 was determined to be 38 nmol/min/mg.

アクセッション番号

Q56UN5

アニマルフリー

No

キャリアフリー

No

種

Human

バッファー組成

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.1% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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組合せ製品

Primary Antibodies

AB32520

Anti-STAT6 antibody [YE361]

20ul selling size|Recombinant|Advanced Validation|RabMAb|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 antibody [YE361] (AB32520)

  • 5
  • 1
Primary Antibodies

AB235418

Anti-KIAA0907 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIAA0907 antibody (AB235418)

  • 0
  • 0
Primary Antibodies

AB282458

Anti-PD-L1 antibody [RM1012]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [RM1012] (AB282458)

  • 0
  • 0
Primary Antibodies

AB263947

Anti-STAT6 (phospho Y641) antibody [EPR22599-78]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Flow Cytometry (Intracellular) - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] (AB263947)

  • 5
  • 1
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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製品の詳細

ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
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配列情報

[{"linker":null,"sequence":"MFGKTLSGTNSISQEIMDSVNNEELTDELLGCLAAELLALDEKDNNSCQKMANETDPENLNLVLRWRGSTPKEMGRETTKVKIQRHSSGLRIYDREEKFLISNEKKIFSENSLKSEEPILWTKGEILGKGAYGTVYCGLTSQGQLIAVKQVALDTSNKLAAEKEYRKLQEEVDLLKALKHVNIVAYLGTCLQENTVSIFMEFVPGGSISSIINRFGPLPEMVFCKYTKQILQGVAYLHENCVVHRDIKGNNVMLMPTGIIKLIDFGCARRLAWAGLNGTHSDMLKSMHGTPYWMAPEVINESGYGRKSDIWSIGCTVFEMATGKPPLASMDRMAAMFYIGAHRGLMPPLPDHFSENAADFVRMCLTRDQHERPSALQLLKHSFLERSH","proteinLength":"Fragment","predictedMolecularWeight":"83 kDa","actualMolecularWeight":null,"aminoAcidEnd":1328,"aminoAcidStart":941,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q56UN5","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification
出荷温度
Dry Ice
短期保存温度
-80°C
長期保存温度
-80°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
True
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Sps1/Ste20 related kinase 4 also known as STK4 or MST1 is a serine/threonine kinase with a molecular mass of approximately 56 kDa. This kinase belongs to the Germinal Center Kinase (GCK) group of the STE20-like kinase family and it plays a significant role in signal transduction processes. STK4 is broadly expressed with prominent presence in tissues like the liver and pancreas. The protein localizes both in the cytoplasm and nucleus and is known to translocate in response to stress signals.
Biological function summary

This kinase helps regulate apoptosis and cell proliferation. As a part of the Hippo signaling pathway STK4/MST1 operates within a cascade of interactions involving multiple cellular proteins. It forms a complex with other proteins such as SAV1 and LATS1/2 to execute control over organ size by modulating cell growth and apoptosis. Through these interactions STK4 maintains cellular homeostasis and prevents abnormal cellular developments such as overgrowth.

Pathways

STK4 plays an integral part in the Hippo signaling and MAPK pathways. Within the Hippo pathway it partners with MST2 to phosphorylate downstream effectors which impacts transcription regulation and ultimately cellular proliferation and apoptosis. In the MAPK pathway STK4’s activities interface with those of the YAP and TAZ proteins further linking it to cellular signaling processes that control cell fate decisions.

Aberrant STK4 activity connects to cancer and neurodegenerative disorders. Its dysfunctional signaling in the Hippo pathway can lead to tumorigenesis especially when downregulation occurs reducing its inhibitory control over cell growth. In the context of neurodegenerative disorders altered STK4 activity associates with conditions like Alzheimer's disease where its interaction with tau protein influences tau phosphorylation and aggregation both hallmarks of the disease.
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一般的な情報

配列の類似性

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

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製品プロトコール

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ターゲットの情報

See full target information MAP3K19
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