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Recombinant Human SPARC protein (ab92965)

Submit a review Q&A (1)
SDS-PAGE - Recombinant Human SPARC protein (ab92965)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 90% SDS-PAGE
    • Suitable for: WB, ELISA, SDS-PAGE, MS

    製品の詳細

    • 製品名

      Recombinant Human SPARC protein
      SPARC タンパク質・ペプチド 製品一覧

    • 精製度

      > 90 % SDS-PAGE.
      Purified by MBP tag / Dextrin Sepharose affinity chromatography.

    • 発現系

      Escherichia coli

    • タンパク質長

      Full length protein

    • Animal free

      No

    • 由来

      Recombinant

      • 生物種

        Human

    関連製品

    特性

    Our Abpromise guarantee covers the use of ab92965 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • アプリケーション

      Western blot

      ELISA

      SDS-PAGE

      Mass Spectrometry

    • 製品の状態

      Lyophilized

    • 備考

      After reconstitution, store in working aliquots at -80ºC.
    • Concentration information loading...

    前処理および保存

    • 保存方法および安定性

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 7.40
      Constituents: 0.316% Tris HCl, 0.0292% EDTA, 1.16% Sodium chloride, 0.342% Maltose

    関連情報

    • 別名

      • AA517111
      • Basement membrane protein 40
      • Basement-membrane protein 40
      • BM 40
      • BM-40
      • BM40
      • Cysteine rich protein
      • hm:zeh0062
      • MGC128090
      • OI17
      • ON
      • Osteonectin
      • Secreted acidic cystein rich glycoprotein
      • Secreted protein acidic and cysteine rich
      • Secreted protein acidic and rich in cysteine
      • Secreted protein acidic cysteine rich
      • Secreted protein acidic cysteine rich (osteonectin)
      • SPARC
      • SPRC
      • SPRC_HUMAN
      see all

    • 機能

      Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.

    • 配列類似性

      Belongs to the SPARC family.
      Contains 1 EF-hand domain.
      Contains 1 follistatin-like domain.
      Contains 1 Kazal-like domain.

    • 発生段階

      Expressed at high levels in tissues undergoing morphogenesis, remodeling and wound repair.

    • 細胞内局在

      Secreted > extracellular space > extracellular matrix > basement membrane. In or around the basement membrane.
    • Information by UniProt

    画像

    • SDS-PAGE - Recombinant Human SPARC protein (ab92965)
      SDS-PAGE - Recombinant Human SPARC protein (ab92965)
      ab92965 analysed by 10% SDS-PAGE stained with Coomassie Blue (CB) and immunoblotted with anti-MBP (WB).

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab92965 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab92965 は論文での使用が確認できていません。

    レビューと Q&A

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    レビューを送る 質問を送る

    Question

    Dear All,



    Are those proteins below injectable in Mice?




    1. SPARC protein (MBP) (ab92965)



    2. TGF beta 1 protein (Active) (ab50036)




    3. VEGF protein (Active) (ab9571)

    Read More
    Answer

    Thank you for contacting us and for your patience.

    I had to contact the laboratory in order to get more information about the use of these three proteins.

    I can confirm, that for the ab92965 SPARC protein (MBP) to our knowledge it has not been tested for injection in mice or other animals (besides it uses as immunogen for polyclonal antibodies production in rabbits intramuscular). It could be that it will work for injection in mice but we can not guarantee this and we have no information about different administration routes (intravenous, intramuscular etc.).

    The two other proteins have been used in mice before. See the following links:

    1. ab9571 VEGF protein (Active)

    http://www.ncbi.nlm.nih.gov/pubmed/19088027

    http://www.ncbi.nlm.nih.gov/pubmed/10784613

    http://www.ncbi.nlm.nih.gov/pubmed/11689883

    http://www.ncbi.nlm.nih.gov/pubmed/12036930

    http://www.ncbi.nlm.nih.gov/pubmed/9401980

    http://www.ncbi.nlm.nih.gov/pubmed/15358628

    http://www.ncbi.nlm.nih.gov/pubmed/16477043

    http://www.ncbi.nlm.nih.gov/pubmed/16888632

    http://www.pnas.org/content/104/8/2837.full

    http://www.ncbi.nlm.nih.gov/pubmed/18180379

    http://www.ncbi.nlm.nih.gov/pubmed/15067321

    http://www.ncbi.nlm.nih.gov/pubmed/20056792

    http://www.ncbi.nlm.nih.gov/pubmed/22025303

    http://www.pnas.org/content/early/2011/07/25/1016546108.abstract



    2. ab50036 TGF beta 1 protein (Active)

    http://www.ncbi.nlm.nih.gov/pubmed/20304823

    http://www.ncbi.nlm.nih.gov/pubmed/22761317

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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