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Recombinant Human SPAM1 protein (Tagged) (ab114725)

Submit a review Q&A (3)
SDS-PAGE - Recombinant Human SPAM1 protein (ab114725)

    Key features and details

    • Expression system: Wheat germ
    • Tags: GST tag N-Terminus
    • Suitable for: ELISA, SDS-PAGE, WB

    製品の詳細

    • 製品名

      Recombinant Human SPAM1 protein (Tagged)
      SPAM1 タンパク質・ペプチド 製品一覧

    • 発現系

      Wheat germ

    • アクセッション番号

      P38567

    • タンパク質長

      Protein fragment

    • Animal free

      No

    • 由来

      Recombinant

      • 生物種

        Human

      • 配列

        RSMKSCLLLDNYMETILNPYIINVTLAAKMCSQVLCQEQGVCIRKNWNSS DYLHLNPDNFAIQLEKGGKFTVRGKPTLEDLEQFSEKFYCSCYSTLSCKE

      • 予測される分子量

        37 kDa including tags

      • 領域

        346 to 445

      • タグ

        GST tag N-Terminus

    特性

    Our Abpromise guarantee covers the use of ab114725 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • アプリケーション

      ELISA

      SDS-PAGE

      Western blot

    • 製品の状態

      Liquid

    • 備考

      This product was previously labelled as Hyaluronidase PH20.

      Protein concentration is above or equal to 0.05 ug/ul.

       

    • Concentration information loading...

    前処理および保存

    • 保存方法および安定性

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.3% Glutathione, 0.79% Tris HCl

    関連情報

    • 別名

      • epididymis secretory sperm binding protein Li 96n
      • HEL-S-96n
      • HYA1
      • HYAL PH-20
      • HYAL PH20
      • Hyal-PH20
      • HYAL1
      • HYAL3
      • HYAL5
      • HYALP_HUMAN
      • Hyaluronidase PH-20
      • Hyaluronoglucosaminidase PH-20
      • MGC26532
      • PH-20 Hyaluronidase
      • PH20
      • PH20 Hyaluronidase
      • SPAG15
      • SPAM-1
      • Spam1
      • Sperm adhesion molecule 1
      • sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding)
      • Sperm surface protein PH-20
      • Sperm surface protein PH20
      • zona pellucida binding
      see all

    • 機能

      Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.

    • 組織特異性

      Testis.

    • 配列類似性

      Belongs to the glycosyl hydrolase 56 family.

    • 翻訳後修飾

      N-glycosylated.

    • 細胞内局在

      Cell membrane.
    • Information by UniProt

    画像

    • SDS-PAGE - Recombinant Human SPAM1 protein (ab114725)
      SDS-PAGE - Recombinant Human SPAM1 protein (ab114725)

      12.5% SDS-PAGE analysis of ab114725 stained with Coomassie Blue.

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab114725 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab114725 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-3 of 3 Abreviews or Q&A

    Question

    Product code: 112304
    Inquiry: I will like to know if is possible that you send me hyaluronidase PH-20 to use in a research project in my group

    Read More
    Answer

    Thank you for contacting us. Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.

    We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

    I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

    Read More

    Question

    concentration de ab114725 lot 1384718 GR98907-1 ?
    0.05 mg/ml comme indiqué sur la fiche technique ou 0.1 mg/ml comme indiqué sur le tube?

    Read More
    Answer

    Merci de nous avoir contactés.

    Le laboratoire vient de me confirmer que la concentration de la protéineHyaluronidase PH20, lot GR98907-1 est de 0.1 mg/ml.

    La concentration de cette protéine dépend du lot, la fiche technique va être mise à jour avec cette information dans les meilleurs délais.

    Nous sommes désolés pour la confusion et espérons que cette information vous sera utile. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

    Read More

    Question

    functional activity?

    Read More
    Answer

    Thank you for contacting us and sorry for the delay of our reply.

    I just received a reply from the source of the Hyaluronidase PH20 protein ab114725. They confirmed that this protein has not been tested for functional studies.
    All tested applications are specified on our datasheets, and these are updated as soon as any new information is brought to our attention.

    If you would like to test the protein in this untested application, please contact our Scientific Support team prior to the purchase by replying to this message as you may be eligible for our testing discount program.

    Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

    To find out more about our Abreview system, please see the following link: https://www.abcam.com/abreviews

    I hope this information is helpful. Please do not hesitate to contact me for any further advice or information in this regard.

    Read More

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