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Recombinant Human SPAM1 protein (ab132258)

Submit a review Q&A (1)References (1)
SDS-PAGE - Recombinant Human SPAM1 protein (ab132258)

    Key features and details

    • Expression system: Wheat germ
    • Suitable for: WB, ELISA, SDS-PAGE

    製品の詳細

    • 製品名

      Recombinant Human SPAM1 protein
      SPAM1 タンパク質・ペプチド 製品一覧

    • 発現系

      Wheat germ

    • アクセッション番号

      P38567

    • タンパク質長

      Full length protein

    • Animal free

      No

    • 由来

      Recombinant

      • 生物種

        Human

      • 配列

        MGVLKFKHIFFRSFVKSSGVSQIVFTFLLIPCCLTLNFRAPPVIPNVPFL WAWNAPSEFCLGKFDEPLDMSLFSFIGSPRINATGQGVTIFYVDRLGYYP YIDSITGVTVNGGIPQKISLQDHLDKAKKDITFYMPVDNLGMAVIDWEEW RPTWARNWKPKDVYKNRSIELVQQQNVQLSLTEATEKAKQEFEKAGKDFL VETIKLGKLLRPNHLWGYYLFPDCYNHHYKKPGYNGSCFNVEIKRNDDLS WLWNESTALYPSIYLNTQQSPVAATLYVRNRVREAIRVSKIPDAKSPLPV FAYTRIVFTDQVLKFLSQDELVYTFGETVALGASGIVIWGTLSIMRSMKS CLLLDNYMETILNPYIINVTLAAKMCSQVLCQEQGVCIRKNWNSSDYLHL NPDNFAIQLEKGGKFTVRGKPTLEDLEQFSEKFYCSCYSTLSCKEKADVK DTDAVDVCIADGVCIDAFLKPPMETEEPQIFYNASPSTLSATMFIVSILF LIISSVASL

      • 予測される分子量

        84 kDa

      • 領域

        1 to 509

    特性

    Our Abpromise guarantee covers the use of ab132258 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • アプリケーション

      Western blot

      ELISA

      SDS-PAGE

    • 製品の状態

      Liquid

    • 備考

      This product was previously labelled as Hyaluronidase PH20.

       

    • Concentration information loading...

    前処理および保存

    • 保存方法および安定性

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.31% Glutathione, 0.79% Tris HCl

    関連情報

    • 別名

      • epididymis secretory sperm binding protein Li 96n
      • HEL-S-96n
      • HYA1
      • HYAL PH-20
      • HYAL PH20
      • Hyal-PH20
      • HYAL1
      • HYAL3
      • HYAL5
      • HYALP_HUMAN
      • Hyaluronidase PH-20
      • Hyaluronoglucosaminidase PH-20
      • MGC26532
      • PH-20 Hyaluronidase
      • PH20
      • PH20 Hyaluronidase
      • SPAG15
      • SPAM-1
      • Spam1
      • Sperm adhesion molecule 1
      • sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding)
      • Sperm surface protein PH-20
      • Sperm surface protein PH20
      • zona pellucida binding
      see all

    • 機能

      Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.

    • 組織特異性

      Testis.

    • 配列類似性

      Belongs to the glycosyl hydrolase 56 family.

    • 翻訳後修飾

      N-glycosylated.

    • 細胞内局在

      Cell membrane.
    • Information by UniProt

    画像

    • SDS-PAGE - Recombinant Human SPAM1 protein (ab132258)
      SDS-PAGE - Recombinant Human SPAM1 protein (ab132258)
      12.5% SDS-PAGE analysis of ab132258 stained with Coomassie Blue.

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (1)

    ab132258 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab132258 は 1 報の論文で使用されています。

    • Ma R  et al. PH20 Inhibits TGFß1-Induced Differentiation of Perimysial Orbital Fibroblasts via Hyaluronan-CD44 Pathway in Thyroid-Associated Ophthalmopathy. Invest Ophthalmol Vis Sci 60:1431-1441 (2019). PubMed: 30947333

    レビューと Q&A

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    レビューを送る 質問を送る

    Question

    Product code: 112304
    Inquiry: I will like to know if is possible that you send me hyaluronidase PH-20 to use in a research project in my group

    Read More
    Answer

    Thank you for contacting us. Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.

    We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

    I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com