Recombinant human SPAK protein (ab107696)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
製品の詳細
-
製品名
Recombinant human SPAK protein -
生理活性
Specific activity: 22 nmol/min/mg
-
精製度
> 95 % SDS-PAGE.
Purification determined by densitometry. Affinity purified. -
発現系
Baculovirus infected Sf9 cells -
アクセッション番号
-
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
-
生物種
Human -
予測される分子量
63 kDa including tags -
領域
1 to 545 -
タグ
His tag N-Terminus
-
関連製品
-
Related Products
-
Substrate reagent
特性
Our Abpromise guarantee covers the use of ab107696 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
アプリケーション
SDS-PAGE
Functional Studies
-
製品の状態
Liquid -
備考
ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
This product was previously labelled as STK39
-
Concentration information loading...
前処理および保存
-
保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.00
Preservative: 1.02% Imidazole
Constituents: 0.00174% PMSF, 0.82% Sodium phosphate, 0.00385% DTT, 25% Glycerol (glycerin, glycerine), 1.74% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
-
別名
- DCHT
- DKFZp686K05124
- OTTHUMP00000165175
see all -
機能
May act as a mediator of stress-activated signals. -
組織特異性
Predominantly expressed in brain and pancreas followed by heart, lung, kidney, skeletal muscle, liver, placenta and testis. -
配列類似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 protein kinase domain. -
ドメイン
PAPA box (proline-alanine repeats) may target the kinase to a specific subcellular location by facilitating interaction with intracellular proteins such as actin or actin-like proteins. -
細胞内局在
Cytoplasm. Nucleus. Nucleus when caspase-cleaved. - Information by UniProt
画像
-
SDS-PAGE - Recombinant human SPAK protein (ab107696)SDS PAGE analysis of ab107696
-
Functional Studies - Recombinant human SPAK protein (ab107696)The specific activity of SPAK (ab107696) was determined to be 1.9 nmol/min/mg as per activity assay protocol.
-
SDS-PAGE - Recombinant human SPAK protein (ab107696)SDS Page analysis of ab107696 -
Functional Studies - Recombinant human SPAK protein (ab107696)ab107696 specific activity determined to be 22 nmol/min/mg
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab107696 は論文での使用が確認できていません。