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Recombinant Human SNAP25 protein (ab74529)

Submit a review Q&A (3)References (2)
SDS-PAGE - Recombinant Human SNAP25 protein (ab74529)
  • Western blot - Recombinant Human SNAP25 protein (ab74529)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 90% SDS-PAGE
  • Suitable for: SDS-PAGE, WB

製品の詳細

  • 製品名

    Recombinant Human SNAP25 protein
    SNAP25 タンパク質・ペプチド 製品一覧

  • 精製度

    > 90 % SDS-PAGE.
    ab74529 was purified by using conventional chromatography techniques.

  • 発現系

    Escherichia coli

  • タンパク質長

    Full length protein

  • Animal free

    No

  • 由来

    Recombinant

    • 生物種

      Human

    • 配列

      MAEDADMRNE LEEMQRRADQ LADESLESTR RMLQLVEESK DAGIRTLVML DEQGEQLERI EEGMDQINKD MKEAEKNLTD LGKFCGLCVC PCNKLKSSDA YKKAWGNNQD GVVASQPARV VDEREQMAIS GGFIRRVTND ARENEMDENL EQVSGIIGNL RHMALDMGNE IDTQNRQIDR IMEKADSNKT RIDEANQRAT KMLGSG

特性

Our Abpromise guarantee covers the use of ab74529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    SDS-PAGE

    Western blot

  • 製品の状態

    Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.0154% DTT, 0.3025% Tris, 10% Glycerol

関連情報

  • 別名

    • bA416N4.2
    • Bdr
    • CMS18
    • dJ1068F16.2
    • FLJ23079
    • HGNC:11132
    • MGC105414
    • MGC139754
    • Resistance to inhibitors of cholinesterase 4 homolog
    • RIC 4
    • RIC-4
    • RIC4
    • SEC 9
    • SEC9
    • SNAP
    • SNAP 25
    • SNAP-25
    • SNAP-25B
    • SNAP25
    • SNP 25
    • SNP25
    • SNP25_HUMAN
    • sp
    • SUP
    • Super protein
    • Synaptosomal associated 25 kDa protein
    • Synaptosomal associated protein
    • Synaptosomal associated protein 25
    • Synaptosomal associated protein 25kDa
    • Synaptosomal-associated 25 kDa protein
    • Synaptosomal-associated protein
    • Synaptosomal-associated protein 25
    • Synaptosomal-associated protein, 25-KD
    see all

  • 機能

    t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF.

  • 組織特異性

    Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.

  • 配列類似性

    Belongs to the SNAP-25 family.
    Contains 2 t-SNARE coiled-coil homology domains.

  • 翻訳後修飾

    Palmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association.

  • 細胞内局在

    Cytoplasm > perinuclear region. Cell membrane. Cell junction > synapse > synaptosome. Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region.
  • Information by UniProt

画像

  • SDS-PAGE - Recombinant Human SNAP25 protein (ab74529)
    SDS-PAGE - Recombinant Human SNAP25 protein (ab74529)
    15% SDS-PAGE showing ab74529 (3μg)
  • Western blot - Recombinant Human SNAP25 protein (ab74529)
    Western blot - Recombinant Human SNAP25 protein (ab74529)
    Anti-SNAP25 antibody (ab41455) at 1 µg/ml + Recombinant Human SNAP25 protein (ab74529) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 1 minute

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (2)

ab74529 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab74529 は 2 報の論文で使用されています。

  • Sinclair LI & Love S Effect of APOE Genotype on Synaptic Proteins in Earlier Adult Life. J Alzheimers Dis 59:1123-1137 (2017). PubMed: 28731446
  • Stura EA  et al. Structural Framework for Covalent Inhibition of Clostridium botulinum Neurotoxin A by Targeting Cys165. J Biol Chem 287:33607-14 (2012). SDS-PAGE . PubMed: 22869371

レビューと Q&A

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1-3 of 3 Abreviews or Q&A

Question

Could you please help me to find the difference between the following four recombinant proteins SNAP25 you are selling?


Catalog numbers are: ab51048, ab87335, ab155885 and ab74529.

Read More
Answer

I have been in contact with our lab and products team and indeed there is a duplication here which we are currently trying to fix.

I can confirm that ab74529 and ab155885 are identical and ab87335 and ab51048 are also identical.

The difference between ab74529/ab155885 and ab87335/ab51048 is that ab87335/ab51048 is His-tagged whereas ab74529/ab155885 are untagged proteins.

ab87335/ab51048- SNAP25 (Synaptosomal-associated protein) Human, His- tag, Recombinant, E.coli, 25.4 kDa (226 aa), confirmed by MALDI-TOF.
The synaptosomal-associated protein (SNAP-25) is an essential component of the core complex that mediates presynaptic vesicle trafficking. Thus, SNAP-25 is directly involved in the release of neurotransmitters. Recombinant human SNAP25, fused to His tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

ab74529/ab155885 - SNAP25,1-206aa, Human, Recombinant, E.coli, no tag, 23 kDa (206 aa), confirmed by MALDI-TOF.

Read More

Question

Customer kindly contacted us regarding ab74529 and the presence of EDTA in the buffer. The customer is attempting to cleave the SNAP25 protein with a zinc-dependent protease, and has attributed a lack of cleavage to EDTA being in the buffer. The customer is wondering if there is a recommended method for removing the EDTA while minimizing loss of the protein.

Read More
Answer


The laboratory said they would remove the EDTA from ab74529 via dialysis.
Alternatively, we have now added the same SNAP25 protein with EDTA removed:
ab155885: https://www.abcam.com/snap25-protein-edta-free-ab155885.html

Read More

Question

Customer kindly contacted us as this product is showing extra bands when run on western.

Read More
Answer

Thank you for contacting us.

I have been in touch with the lab. While they have not run this protein in Western blot, it has been checked in SDS-PAGE and no additional bands have been seen. Would you be willing to provide and image of the western blot and details of your protocol so that we might review these. We do not believe that the addition of more DTT of BME will adversely affect the protein but are unsure whether this will resolve the issue you are facing.

This product is covered by our Abpromise guarantee. We are happy provide scientific support at any time. If you are using the product in species and applications listed on the datasheet and contact us within six months of purchase, we are also happy to replace or refund the product should we not be able to help you to resolve the issue. More information on our Abpromise may be found at the following link:

https://www.abcam.com/index.html?pageconfig=abpromise

I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

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