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AB236192

Recombinant Human SMARCA2 / BRM protein (Tagged)

Recombinant Human SMARCA2 / BRM protein (Tagged)

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Recombinant Human SMARCA2 / BRM protein (Tagged) is a Human Fragment protein, in the 700 to 1216 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

別名を表示する

BAF190B, BRM, SNF2A, SNF2L2, SMARCA2, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 2, SAMRCA2, BRG1-associated factor 190B, Probable global transcription activator SNF2L2, Protein brahma homolog, SNF2-alpha, hBRM

1 Images
SDS-PAGE - Recombinant Human SMARCA2 / BRM protein (Tagged) (AB236192)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human SMARCA2 / BRM protein (Tagged) (AB236192)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis with 5% enrichment gel and 15% separation gel of ab236192.

Key facts

精製度

>90% SDS-PAGE

発現系

Escherichia coli

タグ

6x His tag N-Terminus SUMO tag N-Terminus

アプリケーション

SDS-PAGE

applications

生物活性

No

アクセッション番号

P51531

アニマルフリー

No

キャリアフリー

No

種

Human

バッファー組成

pH: 7.2 - 7.4 Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

storage-buffer

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組合せ製品

Reagents, Controls & Accessories

AB325995

Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands)

SDS-PAGE - Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands) (AB325995)

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Proteins & Peptides

AB84769

Recombinant Human PI 3 Kinase p85 alpha protein (His tag N-Terminus)

Western blot - Recombinant Human PI 3 Kinase p85 alpha protein (AB84769)

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Primary Antibodies

AB110641

Anti-BRG1 antibody [EPNCIR111A]

20ul selling size|BOND RX™ Validated|Recombinant|KO Validated|Advanced Validation|RabMAb

ChIC/CUT&RUN sequencing - Anti-BRG1 antibody [EPNCIR111A] (AB110641)

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Primary Antibodies

AB240648

Anti-SMARCA2 / BRM antibody [EPR23103-44]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCA2 / BRM antibody [EPR23103-44] (AB240648)

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Primary Antibodies

AB57461

Anti-ARID1B antibody [2D2]

KO Validated

Western blot - Anti-ARID1B antibody [2D2] (AB57461)

  • 5
  • 2
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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配列情報

[{"linker":null,"sequence":"SYYTVAHAISERVEKQSALLINGTLKHYQLQGLEWMVSLYNNNLNGILADEMGLGKTIQTIALITYLMEHKRLNGPYLIIVPLSTLSNWTYEFDKWAPSVVKISYKGTPAMRRSLVPQLRSGKFNVLLTTYEYIIKDKHILAKIRWKYMIVDEGHRMKNHHCKLTQVLNTHYVAPRRILLTGTPLQNKLPELWALLNFLLPTIFKSCSTFEQWFNAPFAMTGERVDLNEEETILIIRRLHKVLRPFLLRRLKKEVESQLPEKVEYVIKCDMSALQKILYRHMQAKGILLTDGSEKDKKGKGGAKTLMNTIMQLRKICNHPYMFQHIEESFAEHLGYSNGVINGAELYRASGKFELLDRILPKLRATNHRVLLFCQMTSLMTIMEDYFAFRNFLYLRLDGTTKSEDRAALLKKFNEPGSQYFIFLLSTRAGGLGLNLQAADTVVIFDSDWNPHQDLQAQDRAHRIGQQNEVRVLRLCTVNSVEEKILAAAKYKLNVDQKVIQAGMFDQKSSSHERRAF","proteinLength":"Fragment","predictedMolecularWeight":"75.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":1216,"aminoAcidStart":700,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P51531","tags":[{"tag":"6x His","terminus":"N-Terminus"},{"tag":"SUMO","terminus":"N-Terminus"}]}]
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出荷温度及び保存条件

製品の状態
Liquid
出荷温度
Blue Ice
短期保存温度
-20°C
長期保存温度
-20°C
保管に関する情報
Avoid freeze / thaw cycle
False
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The SMARCA2 protein also known as BRM (Brahma-related gene 1) is a component of the SWI/SNF chromatin remodeling complex. This protein has a molecular mass of approximately 185 kDa. Scientists observe that SMARCA2/BRM is expressed in various tissues including the brain and liver. It acts as an ATPase which moves histone octamers in chromatin and facilitates access to DNA. SMARCA2/BRM plays an important role in transcriptional activation by modifying chromatin structure.
Biological function summary

SMARCA2/BRM interacts with other subunits to form the SWI/SNF complex important for regulating gene expression. This complex helps control the transcription of specific genes by repositioning nucleosomes. SMARCA2/BRM modulates cell cycle progression and differentiation as it influences transcription factor access to DNA. Its activity is essential for normal cellular responses maintaining cell homeostasis and development.

Pathways

SMARCA2/BRM participates significantly in the cell cycle and Wnt signaling pathways. In the cell cycle it cooperates with proteins like p53 to regulate genes involved in cell growth and apoptosis. In the Wnt pathway SMARCA2/BRM interacts with beta-catenin to influence cellular responses to Wnt signals. These pathways are critical for cell proliferation and maintaining cellular integrity and SMARCA2/BRM serves as a pivotal modulator within these systems.

SMARCA2/BRM has a connection to cancer and Coffin-Siris syndrome. Altered expression of SMARCA2/BRM has been observed in several cancers where it frequently interacts with other oncogenic proteins disrupting normal regulatory functions and promoting tumorigenesis. Meanwhile mutations in SMARCA2 can lead to Coffin-Siris syndrome a genetic disorder affecting development and it is connected with other proteins like SOX2 which are also involved in developmental pathways. Understanding SMARCA2's role may uncover insights into therapeutic targets for these conditions.
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一般的な情報

機能

ATPase involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Binds DNA non-specifically (PubMed : 15075294, PubMed : 22952240, PubMed : 26601204). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity).

配列の類似性

Belongs to the SNF2/RAD54 helicase family.

翻訳後修飾

During apoptosis, cleaved by cathepsin CTSG to produce a 160 kDa cleavage product which localizes to the cytosol.. Ubiquitinated.

細胞内局在性

Nucleus

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製品プロトコール

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ターゲットの情報

ATPase involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Binds DNA non-specifically (PubMed : 15075294, PubMed : 22952240, PubMed : 26601204). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity).
See full target information SMARCA2
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