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Recombinant Human SMAD5 protein (ab84651)

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SDS-PAGE - Recombinant Human SMAD5 protein (ab84651)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 85% Densitometry
    • Suitable for: WB, SDS-PAGE

    製品の詳細

    • 製品名

      Recombinant Human SMAD5 protein

    • 精製度

      > 85 % Densitometry.

    • 発現系

      Escherichia coli

    • タンパク質長

      Full length protein

    • Animal free

      No

    • 由来

      Recombinant

      • 生物種

        Human

    特性

    Our Abpromise guarantee covers the use of ab84651 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • アプリケーション

      Western blot

      SDS-PAGE

    • 製品の状態

      Liquid
    • Concentration information loading...

    前処理および保存

    • 保存方法および安定性

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 7.50
      Constituents: 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

    関連情報

    • 別名

      • DKFZp781C1895
      • DKFZp781O1323
      • Dwfc
      • hSmad5
      • JV5 1
      • JV5-1
      • MAD homolog 5
      • MAD, mothers against decapentaplegic homolog 5
      • MADH 5
      • MADH5
      • Mothers against decapentaplegic homolog 5
      • mothers against decapentaplegic, drosophila, homolog of, 5
      • Mothers against DPP homolog 5
      • MusMLP
      • SMA and MAD related protein 5
      • SMAD 5
      • SMAD family member 5
      • SMAD, mothers against DPP homolog 5
      • Smad5
      • SMAD5_HUMAN
      see all

    • 機能

      Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).

    • 組織特異性

      Ubiquitous.

    • 配列類似性

      Belongs to the dwarfin/SMAD family.
      Contains 1 MH1 (MAD homology 1) domain.
      Contains 1 MH2 (MAD homology 2) domain.

    • 翻訳後修飾

      Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase.
      Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.

    • 細胞内局在

      Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.
    • Information by UniProt

    画像

    • SDS-PAGE - Recombinant Human SMAD5 protein (ab84651)
      SDS-PAGE - Recombinant Human SMAD5 protein (ab84651)
      SDS-PAGE showing ab84651 at approximately 82kDa.

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab84651 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab84651 は論文での使用が確認できていません。

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