AB268899

Recombinant human SHP2 protein (Active)

Name: Recombinant human SHP2 protein (Active) (ab268899) | Abcam
Brand: Abcam Limited
SKU: AB268899
Price: 49000 JPY
Availability: InStock

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Recombinant human SHP2 protein (Active) is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS.

別名を表示する

PTP2C, SHPTP2, PTPN11, Tyrosine-protein phosphatase non-receptor type 11, Protein-tyrosine phosphatase 1D, Protein-tyrosine phosphatase 2C, SH-PTP2, SH-PTP3, PTP-1D, PTP-2C, SHP-2, Shp2

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Functional Studies - Recombinant human SHP2 protein (Active) (AB268899)

FuncS

Supplier Data

Functional Studies - Recombinant human SHP2 protein (Active) (AB268899)

The specific activity of ab268899 was 7,800 nmol/min/mg in a phosphatase assay using tyrosine phosphopeptide-2 (DADE(pY)LIPQQG) as substrate.

SDS-PAGE - Recombinant human SHP2 protein (Active) (AB268899)

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human SHP2 protein (Active) (AB268899)

SDS-PAGE analysis of ab268899.

Key facts

精製度

>90% SDS-PAGE

発現系

Baculovirus infected Sf9 cells

アプリケーション

SDS-PAGE, FuncS

applications

生物活性

Yes

生物学的活性

The specific activity of ab268899 was 7,800 nmol/min/mg in a phosphatase assay using tyrosine phosphopeptide-2 (DADE(pY)LIPQQG) as substrate..

アクセッション番号

Q06124

アニマルフリー

キャリアフリー

種

Human

バッファー組成

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.42% MOPS, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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配列情報

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q06124","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]

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出荷温度及び保存条件

出荷温度

Dry Ice

短期保存温度

-80°C

長期保存温度

-80°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

True

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

SHP2 also known as PTPN11 is a protein tyrosine phosphatase with a molecular mass of approximately 68 kDa. It is expressed in various tissues including the heart liver and immune cells. SHP2 belongs to the non-receptor class of protein tyrosine phosphatases and plays a critical role in cell signaling by acting as a regulator of signal transduction processes. SHP2 mediates these processes by dephosphorylating specific phosphotyrosine residues on target proteins influencing various cellular functions like proliferation differentiation and survival.

Biological function summary

The role of SHP2 extends to involvement in several signaling cascades such as the Ras/MAPK and PI3K/AKT pathways. It functions as an essential component within protein complexes that facilitate cell communication and response to external signals. The protein modulates growth factor signaling and cytokine signaling highlighting its significance in normal cell function and development. SHP2's statement in signaling processes makes it an important regulator of cellular dynamics.

Pathways

SHP2 participates in the Ras/MAPK and PI3K/AKT signaling pathways which are important for regulating cell growth survival and differentiation. Within these pathways SHP2 interacts with various signaling molecules including Grb2 Sos and Gab family adaptors. These interactions coordinate cellular responses to growth factors and other extracellular cues ensuring proper pathway activation and control. By serving as a critical mediator SHP2 integrates signals that are necessary for appropriate cellular outcomes.

SHP2 is associated with several conditions such as Noonan syndrome and various cancers. Mutations in the PTPN11 gene which encodes SHP2 often result in aberrant signaling that leads to developmental anomalies or tumorigenesis. In Noonan syndrome the mutated SHP2 protein results in disrupted Ras/MAPK pathway signaling. As for cancers SHP2 is often found to be overactive leading to enhanced cell proliferation and survival. In these contexts SHP2 is interconnected with other proteins like RAS and RAF which also contribute to oncogenic pathway activation and disease progression.

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製品の性状

製品の状態

Liquid

補足情報

Affinity purified.

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一般的な情報

機能

Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus (PubMed : 10655584, PubMed : 14739280, PubMed : 18559669, PubMed : 18829466, PubMed : 26742426, PubMed : 28074573, PubMed : 32184441). Positively regulates MAPK signal transduction pathway (PubMed : 28074573). Dephosphorylates GAB1, ARHGAP35 and EGFR (PubMed : 28074573). Dephosphorylates ROCK2 at 'Tyr-722' resulting in stimulation of its RhoA binding activity (PubMed : 18559669). Dephosphorylates CDC73 (PubMed : 26742426). Dephosphorylates SOX9 on tyrosine residues, leading to inactivate SOX9 and promote ossification (By similarity). Dephosphorylates tyrosine-phosphorylated NEDD9/CAS-L (PubMed : 19275884). Acts as an effector of PDCD1-mediated inhibition of T-cell response : recruited by phosphorylated PDCD1, mediating dephosphorylation of key T-cell receptor (TCR) proximal signaling molecules, leading to TCR signaling inhibition (PubMed : 32184441).

配列の類似性

Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.

翻訳後修飾

Phosphorylated on Tyr-542 and Tyr-580 upon receptor protein tyrosine kinase activation; which creates a binding site for GRB2 and other SH2-containing proteins. Phosphorylated upon activation of the receptor-type kinase FLT3. Phosphorylated upon activation of the receptor-type kinase PDGFRA (By similarity). Phosphorylated by activated PDGFRB.

細胞内局在性

Nucleus

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information PTPN11

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Recombinant human SHP2 protein (Active)