Recombinant Human SFRP4 protein (Active) (ab288790)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Endotoxin level: <= 0.005 Eu/µg
- Active: Yes
製品の詳細
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製品名
Recombinant Human SFRP4 protein (Active) -
生理活性
Fully biologically active determined by the dose dependent inhibition of WNT-3a induced Wnt/ß-catenin activation in HEK293 TCF/LEF luciferase reporter cell line. ED50 is ≤ 52.4 ng/ml, corresponding to a specific activity of 1.9 x 104 units/mg.
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精製度
>= 95 % SDS-PAGE. -
エンドトキシン・レベル
<=0.005 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
Yes -
由来
Recombinant -
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生物種
Human -
配列
VRGAPCEAVRIPMCRHMPWNITRMPNHLHHSTQENAILAIEQYEELVDVN CSAVLRFFLCAMYAPICTLEFLHDPIKPCKSVCQRARDDCEPLMKMYNHS WPESLACDELPVYDRGVCISPEAIVTDLPEDVKWIDITPDMMVQERPLDV DCKRLSPDRCKCKKVKPTLATYLSKNYSYVIHAKIKAVQRSGCNEVTTVV DVKEIFKSSSPIPRTQVPLITNSSCQCPHILPHQDVLIMCYEWRSRMMLL ENCLVEKWRDQLSKRSIQWEERLQEQRRTVQDKKKTAGRTSRSNPPKPKG KPPAPKPASPKKNIKTRSAQKRTNPKRV -
予測される分子量
40 kDa -
領域
19 to 346 -
配列の追加情報
N-terminal glycine
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特性
Our Abpromise guarantee covers the use of ab288790 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at Room Temperature. Store at Room Temperature.
pH: 7.40
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Monobasic dihydrogen potassium phosphateThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. Store lyophilized form at room temperature. Reconstitute in phosphate buffered saline, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw.
関連情報
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別名
- Frizzled protein
- Frizzled protein human endometrium
- FRP 4
see all -
機能
Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP4 may act as a regulator of adult uterine morphology and function. Increases apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling (By similarity). Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake. -
組織特異性
Expressed in mesenchymal cells. Highly expressed in the stroma of proliferative endometrium. Expressed in cardiomyocytes. Shows moderate to strong expression in ovarian tumors with expression increasing as the tumor stage increases. In ovarian tumors, expression levels are inversely correlated with expression of CTNNB1 (at protein level). -
配列類似性
Belongs to the secreted frizzled-related protein (sFRP) family.
Contains 1 FZ (frizzled) domain.
Contains 1 NTR domain. -
ドメイン
The FZ domain is involved in binding with Wnt ligands. -
細胞内局在
Secreted. Cytoplasmic in ovarian tumor cells. - Information by UniProt
画像
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Biological Activity - Recombinant Human SFRP4 protein (Active) (ab288790)
Fully biologically active determined by the dose dependent inhibition of WNT-3a induced Wnt/ß-catenin activation in HEK293 TCF/LEF luciferase reporter cell line. ED50 is ≤ 52.4 ng/ml, corresponding to a specific activity of 1.9 x 104 units/mg.
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SDS-PAGE - Recombinant Human SFRP4 protein (ab288790)SDS-PAGE analysis of ab288790 -
Mass Spectrometry - Recombinant Human SFRP4 protein (ab288790)Mass determination by ESI-TOF.
Predicted MW is 37888.18 Da. (+/- 10 Da by ESI-TOF). Observed MW is 37893.90 Da.
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HPLC - Recombinant Human SFRP4 protein (ab288790)HPLC analysis of ab288790
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab288790 は論文での使用が確認できていません。
