Recombinant Human SFRP1 protein (Active) (ab288782)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Active: Yes
製品の詳細
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製品名
Recombinant Human SFRP1 protein (Active)
SFRP1 タンパク質・ペプチド 製品一覧 -
生理活性
Fully biologically active determined by the dose dependent inhibition of WNT-3a induced Wnt/ß-catenin activation in HEK293 TCF/LEF luciferase reporter cell line. ED50 is ≤ 330.5 ng/ml, corresponding to a specific activity of 3.0 x 103 units/mg.
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精製度
>= 95 % SDS-PAGE. -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
Yes -
由来
Recombinant -
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生物種
Human -
配列
SEYDYVSFQSDIGPYQSGRFYTKPPQCVDIPADLRLCHNVGYKKMVLPNL LEHETMAEVKQQASSWVPLLNKNCHAGTQVFLCSLFAPVCLDRPIYPCRW LCEAVRDSCEPVMQFFGFYWPEMLKCDKFPEGDVCIAMTPPNATEASKPQ GTTVCPPCDNELKSEAIIEHLCASEFALRMKIKEVKKENGDKKIVPKKKK PLKLGPIKKKDLKKLVLYLKNGADCPCHQLDNLSHHFLIMGRKVKSQYLL TAIHKWDKKNKEFKNFMKKMKNHECPTFQSVFK -
予測される分子量
35 kDa -
領域
32 to 314 -
配列の追加情報
N-terminal glycine
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特性
Our Abpromise guarantee covers the use of ab288782 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at Room Temperature. Store at Room Temperature.
pH: 7.40
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Monobasic dihydrogen potassium phosphateThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. Store lyophilized form at room temperature. Reconstitute in phosphate buffered saline, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw.
関連情報
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別名
- Frizzled related protein 1
- FRP
- FRP 1
see all -
機能
Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP1 decreases intracellular beta-catenin levels (By similarity). Has antiproliferative effects on vascular cells, in vitro and in vivo, and can induce, in vivo, an angiogenic response. In vascular cell cycle, delays the G1 phase and entry into the S phase (By similarity). In kidney development, inhibits tubule formation and bud growth in metanephroi (By similarity). Inhibits WNT1/WNT4-mediated TCF-dependent transcription. -
組織特異性
Widely expressed. Absent from lung, liver and peripheral blood leukocytes. Highest levels in heart and fetal kidney. Also expressed in testis, ovary, fetal brain and lung, leiomyomal cells, myometrial cells and vascular smooth muscle cells. Expressed in foreskin fibroblasts and in keratinocytes. -
配列類似性
Belongs to the secreted frizzled-related protein (sFRP) family.
Contains 1 FZ (frizzled) domain.
Contains 1 NTR domain. -
ドメイン
The FZ domain is involved in binding with Wnt ligands. -
細胞内局在
Secreted. Cell membrane or extracellular matrix-associated. Released by heparin-binding. - Information by UniProt
画像
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Biological Activity - Recombinant Human SFRP1 protein (ab288782)
Fully biologically active determined by the dose dependent inhibition of WNT-3a induced Wnt/ß-catenin activation in HEK293 TCF/LEF luciferase reporter cell line. ED50 is ≤ 330.5 ng/ml, corresponding to a specific activity of 3.0 x 103 units/mg.
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SDS-PAGE - Recombinant Human SFRP1 protein (ab288782)SDS-PAGE analysis of ab288782
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab288782 は論文での使用が確認できていません。
