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AB282405

Recombinant Human SERPINE1 Protein (Active)

Recombinant Human SERPINE1 Protein (Active)

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Recombinant Human SERPINE1 Protein (Active) is a Human Full Length protein, in the 24 to 402 aa range, expressed in HEK 293 cells, with >95%, >0.005 EU/µg endotoxin level, suitable for SDS-PAGE, Mass Spec, HPLC, FuncS.

別名を表示する

PAI1, PLANH1, SERPINE1, Plasminogen activator inhibitor 1, PAI, PAI-1, Endothelial plasminogen activator inhibitor, Serpin E1

4 Images
Functional Studies - Recombinant Human SERPINE1 Protein (Active) (AB282405)
  • FuncS

Unknown

Functional Studies - Recombinant Human SERPINE1 Protein (Active) (AB282405)

Fully active determined by its ability to inhibit PLAU mediated cleavage of a peptide substrate N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). ED50 for this effect is ≤1.57 ug/ml corresponding to specific activity of 6.36x103 units/mg.

Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.

Lot GR3396380-2

Mass Spectrometry - Recombinant Human SERPINE1 Protein (Active) (AB282405)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant Human SERPINE1 Protein (Active) (AB282405)

Mass determination by ESI-TOF.

Predicted MW is 42826.25 Da (+/- 10 Da by ESI-TOF). Observed MW is 42828.92

HPLC - Recombinant Human SERPINE1 Protein (Active) (AB282405)
  • HPLC

Supplier Data

HPLC - Recombinant Human SERPINE1 Protein (Active) (AB282405)

HPLC analysis of ab282405.

SDS-PAGE - Recombinant Human SERPINE1 Protein (Active) (AB282405)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human SERPINE1 Protein (Active) (AB282405)

SDS-page analysis of ab282405

Key facts

精製度

>95% HPLC

エンドトキシンレベル

>0.005 EU/µg

発現系

HEK 293 cells

タグ

Tag free

アプリケーション

Mass Spec, HPLC, SDS-PAGE, FuncS

applications

生物活性

Yes

生物学的活性

Fully active determined by its ability to inhibit PLAU mediated cleavage of a peptide substrate N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). ED50 for this effect is ≤1.57 ug/ml corresponding to specific activity of 6.36x103 units/mg.

アクセッション番号

P05121

アニマルフリー

Yes

キャリアフリー

No

種

Human

再構成

Reconstitute in PBS

バッファー組成

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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組合せ製品

Reagents, Controls & Accessories

AB313995

Prestained Protein Ladder - Mid-range molecular weight (15 - 170 kDa)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (15 - 170 kDa) (AB313995)

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Primary Antibodies

AB317604

Anti-PAI1 antibody [EPR21850-262]

BOND RX™ Validated|20ul selling size|Recombinant|RabMAb|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAI1 antibody [EPR21850-262] (AB317604)

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Proteins & Peptides

AB280330

Recombinant human Apolipoprotein E3

Mass Spectrometry - Recombinant human Apolipoprotein E3 (AB280330)

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  • 0
Assay Kits

AB269373

Human PAI1 ELISA Kit (SERPINE1)

Recombinant|SimpleStep

ELISA - Human PAI1 ELISA Kit (SERPINE1) (AB269373)

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  • 1
Proteins & Peptides

AB59967

Recombinant Human RBP4 protein (Tag Free)

Western blot - Recombinant Human RBP4 protein (Tag Free) (AB59967)

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  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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配列情報

[{"linker":null,"sequence":"VHHPPSYVAHLASDFGVRVFQQVAQASKDRNVVFSPYGVASVLAMLQLTTGGETQQQIQAAMGFKIDDKGMAPALRHLYKELMGPWNKDEISTTDAIFVQRDLKLVQGFMPHFFRLFRSTVKQVDFSEVERARFIINDWVKTHTKGMISNLLGKGAVDQLTRLVLVNALYFNGQWKTPFPDSSTHRRLFHKSDGSTVSVPMMAQTNKFNYTEFTTPDGHYYDILELPYHGDTLSMFIAAPYEKEVPLSALTNILSAQLISHWKGNMTRLPRLLVLPKFSLETEVDLRKPLENLGMTDMFRQFQADFTSLSDQEPLHVAQALQKVKIEVNESGTVASSSTAVIVSARMAPEEIIMDRPFLFVVRHNPTGTVLFMGQVMEP","proteinLength":"Full Length","predictedMolecularWeight":"42.83 kDa","actualMolecularWeight":null,"aminoAcidEnd":402,"aminoAcidStart":24,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P05121","tags":[]}]
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出荷温度及び保存条件

製品の状態
Lyophilized
出荷温度
Ambient - Can Ship with Ice
短期保存温度
Ambient
長期保存温度
Ambient
True
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一般的な情報

機能

Serine protease inhibitor. Inhibits TMPRSS7 (PubMed : 15853774). Is a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). As PLAT inhibitor, it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots (PubMed : 17912461, PubMed : 8481516, PubMed : 9207454, PubMed : 21925150). As PLAU inhibitor, it is involved in the regulation of cell adhesion and spreading (PubMed : 9175705). Acts as a regulator of cell migration, independently of its role as protease inhibitor (PubMed : 15001579, PubMed : 9168821). It is required for stimulation of keratinocyte migration during cutaneous injury repair (PubMed : 18386027). It is involved in cellular and replicative senescence (PubMed : 16862142). Plays a role in alveolar type 2 cells senescence in the lung (By similarity). Is involved in the regulation of cementogenic differentiation of periodontal ligament stem cells, and regulates odontoblast differentiation and dentin formation during odontogenesis (PubMed : 25808697, PubMed : 27046084).

配列の類似性

Belongs to the serpin family.

翻訳後修飾

Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-|-Met-370 bond.

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製品プロトコール

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ターゲットの情報

Serine protease inhibitor. Inhibits TMPRSS7 (PubMed : 15853774). Is a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). As PLAT inhibitor, it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots (PubMed : 17912461, PubMed : 8481516, PubMed : 9207454, PubMed : 21925150). As PLAU inhibitor, it is involved in the regulation of cell adhesion and spreading (PubMed : 9175705). Acts as a regulator of cell migration, independently of its role as protease inhibitor (PubMed : 15001579, PubMed : 9168821). It is required for stimulation of keratinocyte migration during cutaneous injury repair (PubMed : 18386027). It is involved in cellular and replicative senescence (PubMed : 16862142). Plays a role in alveolar type 2 cells senescence in the lung (By similarity). Is involved in the regulation of cementogenic differentiation of periodontal ligament stem cells, and regulates odontoblast differentiation and dentin formation during odontogenesis (PubMed : 25808697, PubMed : 27046084).
See full target information SERPINE1
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