Recombinant Human SAT1 protein (ab87666)

Thank you very much for contacting us with the concern of this customer. Indeed, the picture on the website is not a WEstern blot, however a SDS page where the 3ug of the protein have been loaded and than stained with coomassie blue. If the customer gets only a faint band with a coomassie blue staining, I think this is expected if only 0.1ug are loaded. It is 30 times less. If the customer however is running a Western blot, where the protein is detected with an antibody, I would expect a stronger band if the antibody is working well. Please let me know if the customer is running a Western blot, in that case I would recommend to fill the questionnaire so I can try to help to troubleshoot. If the customer is concerned that not enough protein is in the vial, I would recommend firstly to measure the concentration of the protein. This can either be done by a BSA assay, OD measurement or running a protein sample of known concentration (e.g. BSA) as a standard on the SDS Page. I hope this information is helpful. Please let me know if you have any further questions.