Recombinant human RSPO1 protein (Active) (ab246420)
Key features and details
- Expression system: CHO cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant human RSPO1 protein (Active)
RSPO1 タンパク質・ペプチド 製品一覧 -
生理活性
Luciferase induction in HEK-293 STF cells in the presence of Murine Wnt-3a. ED50 ≤ 50 ng/mL (≥ 2.0 x 104 units/mg).
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精製度
> 95 % SDS-PAGE. -
エンドトキシン・レベル
< 1.000 Eu/µg -
発現系
CHO cells -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
RISAEGSQACAKGCELCSEVNGCLKCSPKLFILLERNDIRQVGVCLPSCP PGYFDARNPDMNKCIKCKIEHCEACFSHNFCTKCKEGLYLHKGRCYPACP EGSSAANGTMECSSPAQCEMSEWSPWGPCSKKQQLCGFRRGSEERTRRVL HAPVGDHAACSDTKETRRCTVRRVPCPEGQKRRKGGQGRRENANRNLARK ESKEAGAGSRRRKGQQQQQQQGTVGPLTSAGPA -
予測される分子量
26 kDa -
領域
31 to 263 -
配列の追加情報
Source: Genetically modified CHO cells.
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特性
Our Abpromise guarantee covers the use of ab246420 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon reconsitution add a carrier protein (0.1% BSA). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituent: PBS
0.2 micron filteredThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Sterile water at 0.1 mg/mL. Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C, and avoid repeat freeze thaws.
関連情報
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別名
- CRISTIN3
- FLJ40906
- hRspo1
see all -
機能
Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway, possibly via a direct interaction with Wnt proteins, and in a Wnt-independent beta catenin pathway through a receptor signaling pathway that may not use frizzled/LRP receptors. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination. -
組織特異性
Abundantly expressed in adrenal glands, ovary, testis, thyroid and trachea but not in bone marrow, spinal cord, stomach, leukocytes colon, small intestine, prostate, thymus and spleen. -
関連疾患
Defects in RSPO1 are the cause of palmoplantar keratoderma with squamous cell carcinoma of skin and sex reversal (PKKSCC) [MIM:610644]. This recessive syndrome is characterized by XX (female to male) SRY-independent sex reversal, palmoplantar hyperkeratosis and predisposition to squamous cell carcinoma of the skin. -
配列類似性
Belongs to the R-spondin family.
Contains 2 FU (furin-like) repeats.
Contains 1 TSP type-1 domain. -
ドメイン
The FU repeats are required for activation and stabilization of beta-catenin. -
細胞内局在
Secreted. - Information by UniProt
画像
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SDS-PAGE - Recombinant human RSPO1 protein (Active) (ab246420)
SDS-PAGE abalysis of ab246420 (1 μg per lane) under reducing (Lane 1) and non-reducing (Lane 2) conditions.
ab246420 runs at a higher molecular weight than predicted (25.6 kDa) due to glycosylation.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab246420 は論文での使用が確認できていません。
