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AB123171

Recombinant Human RRAGC protein (His tag N-Terminus)

Recombinant Human RRAGC protein (His tag N-Terminus)

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Recombinant Human RRAGC protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 399 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE, Mass Spec.

別名を表示する

Ras-related GTP-binding protein C, Rag C, RagC, GTPase-interacting protein 2, TIB929, RRAGC

1 Images
SDS-PAGE - Recombinant Human RRAGC protein (His tag N-Terminus) (AB123171)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human RRAGC protein (His tag N-Terminus) (AB123171)

15% SDS-PAGE showing ab123171 (3µg).

Key facts

精製度

>85% SDS-PAGE

発現系

Escherichia coli

タグ

His tag N-Terminus

アプリケーション

Mass Spec, SDS-PAGE

applications

生物活性

No

アクセッション番号

Q9HB90

アニマルフリー

No

キャリアフリー

No

種

Human

バッファー組成

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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配列情報

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSHMSLQYGAEETPLAGSYGAADSFPKDFGYGVEEEEEEAAAAGGGVGAGAGGGCGPGGADSSKPRILLMGLRRSGKSSIQKVVFHKMSPNETLFLESTNKIYKDDISNSSFVNFQIWDFPGQMDFFDPTFDYEMIFRGTGALIYVIDAQDDYMEALTRLHITVSKAYKVNPDMNFEVFIHKVDGLSDDHKIETQRDIHQRANDDLADAGLEKLHLSFYLTSIYDHSIFEAFSKVVQKLIPQLPTLENLLNIFISNSGIEKAFLFDVVSKIYIATDSSPVDMQSYELCCDMIDVVIDVSCIYGLKEDGSGSAYDKESMAIIKLNNTTVLYLKEVTKFLALVCILREESFERKGLIDYNFHCFRKAIHEVFEVGVTSHRSCGHQTSASSLKALTHNGTPRNAI","proteinLength":"Full Length","predictedMolecularWeight":"46.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":399,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9HB90","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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出荷温度及び保存条件

出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
False
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The RRAGC protein also known as Ras-related GTP-binding protein C weighs approximately 44.5 kDa. It functions mechanically as a GTPase cycling between active GTP-bound and inactive GDP-bound states. This protein is expressed in various tissues with noticeable presence in the brain and liver. RRAGC plays a role in cellular signaling and growth mechanisms by interacting with other members of the Ras superfamily.
Biological function summary

RRAGC forms a part of the heterodimeric Rag GTPase complex often teaming with its partner RRAGB. This complex acts as an important regulator in the mTORC1 signaling pathway influencing nutrient sensing and energy balance within cells. RRAGC's ability to toggle between different energy states allows it to contribute to cellular metabolism and response to external stress.

Pathways

RRAGC engages directly in the mTOR signaling pathway an important driver of cell growth and proliferation. It interacts closely with related proteins such as Raptor and mTOR kinase forming a bridge between amino acid availability and cellular growth signals. Additionally RRAGC's role connects to the autophagy pathway affecting cellular degradation processes and nutrient recycling under nutrient-deprived conditions.

The dysregulation of RRAGC's activity associates with conditions like cancer and neurodegenerative diseases. Alterations in its function may link to aberrant mTOR signaling contributing to uncontrolled cellular proliferation seen in cancers. Furthermore RRAGC and its interactions with proteins such as TSC2 (Tuberous Sclerosis Complex 2) highlight potential influences in neurodegenerative disease pathways shedding light on its involvement in conditions linked to cellular growth dysregulation.
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製品の性状

製品の状態

Liquid

補足情報

ab123171 is purified using conventional chromatography techniques.

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一般的な情報

機能

Guanine nucleotide-binding protein that plays a crucial role in the cellular response to amino acid availability through regulation of the mTORC1 signaling cascade (PubMed : 20381137, PubMed : 24095279, PubMed : 27234373, PubMed : 31601708, PubMed : 31601764, PubMed : 32612235, PubMed : 34071043, PubMed : 36697823, PubMed : 37057673). Forms heterodimeric Rag complexes with RagA/RRAGA or RagB/RRAGB and cycles between an inactive GTP-bound and an active GDP-bound form : RagC/RRAGC is in its active form when GDP-bound RagC/RRAGC forms a complex with GTP-bound RagA/RRAGA (or RagB/RRAGB) and in an inactive form when GTP-bound RagC/RRAGC heterodimerizes with GDP-bound RagA/RRAGA (or RagB/RRAGB) (PubMed : 24095279, PubMed : 31601708, PubMed : 31601764, PubMed : 32868926). In its GDP-bound active form, promotes the recruitment of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB (PubMed : 20381137, PubMed : 24095279, PubMed : 27234373, PubMed : 32612235, PubMed : 36697823). This is a crucial step in the activation of the MTOR signaling cascade by amino acids (PubMed : 20381137, PubMed : 24095279, PubMed : 27234373). Also plays a central role in the non-canonical mTORC1 complex, which acts independently of RHEB and specifically mediates phosphorylation of MiT/TFE factors TFEB and TFE3 : GDP-bound RagC/RRAGC mediates recruitment of MiT/TFE factors TFEB and TFE3 (PubMed : 32612235, PubMed : 36697823).

配列の類似性

Belongs to the GTR/RAG GTP-binding protein family.

細胞内局在性

Nucleus

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製品プロトコール

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ターゲットの情報

Guanine nucleotide-binding protein that plays a crucial role in the cellular response to amino acid availability through regulation of the mTORC1 signaling cascade (PubMed : 20381137, PubMed : 24095279, PubMed : 27234373, PubMed : 31601708, PubMed : 31601764, PubMed : 32612235, PubMed : 34071043, PubMed : 36697823, PubMed : 37057673). Forms heterodimeric Rag complexes with RagA/RRAGA or RagB/RRAGB and cycles between an inactive GTP-bound and an active GDP-bound form : RagC/RRAGC is in its active form when GDP-bound RagC/RRAGC forms a complex with GTP-bound RagA/RRAGA (or RagB/RRAGB) and in an inactive form when GTP-bound RagC/RRAGC heterodimerizes with GDP-bound RagA/RRAGA (or RagB/RRAGB) (PubMed : 24095279, PubMed : 31601708, PubMed : 31601764, PubMed : 32868926). In its GDP-bound active form, promotes the recruitment of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB (PubMed : 20381137, PubMed : 24095279, PubMed : 27234373, PubMed : 32612235, PubMed : 36697823). This is a crucial step in the activation of the MTOR signaling cascade by amino acids (PubMed : 20381137, PubMed : 24095279, PubMed : 27234373). Also plays a central role in the non-canonical mTORC1 complex, which acts independently of RHEB and specifically mediates phosphorylation of MiT/TFE factors TFEB and TFE3 : GDP-bound RagC/RRAGC mediates recruitment of MiT/TFE factors TFEB and TFE3 (PubMed : 32612235, PubMed : 36697823).
See full target information Ras-related GTP-binding protein C
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