AB140736

Recombinant Human RPS10 protein (His tag N-Terminus)

Name: Recombinant Human RPS10 protein (ab140736) | Abcam
Brand: Abcam Limited
SKU: AB140736
Price: 88000 JPY
Availability: InStock

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Recombinant Human RPS10 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 165 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE, Mass Spec.

別名を表示する

Small ribosomal subunit protein eS10, 40S ribosomal protein S10, RPS10

1 Images

SDS-PAGE - Recombinant Human RPS10 protein (His tag N-Terminus) (AB140736)

SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human RPS10 protein (His tag N-Terminus) (AB140736)

15% SDS-PAGE analysis of ab140736 (3 µg).

Key facts

精製度

>85% SDS-PAGE

発現系

Escherichia coli

アプリケーション

SDS-PAGE, Mass Spec

applications

生物活性

アクセッション番号

P46783

アニマルフリー

キャリアフリー

種

Human

バッファー組成

pH: 8 Constituents: 30% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

This product was previously labelled as Ribosomal protein S10

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配列情報

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMLMPKKNRIAIYELLFKEGVMVAKKDVHMPKHPELADKNVPNLHVMKAMQSLKSRGYVKEQFAWRHFYWYLTNEGIQYLRDYLHLPPEIVPATLRRSRPETGRPRPKGLEGERPARLTRGEADRDTYRRSAVPPGADKKAEAGAGSATEFQFRGGFGRGRGQPPQ","proteinLength":"Full Length","predictedMolecularWeight":"21 kDa","actualMolecularWeight":null,"aminoAcidEnd":165,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P46783","tags":[{"tag":"His","terminus":"N-Terminus"}]}]

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

False

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

RPS10 also known as 40S ribosomal protein S10 is a component of the small ribosomal subunit essential for protein synthesis. It weighs approximately 18.7 kDa. The protein is expressed in various tissues including the liver kidney and brain. Its primary role involves participating in the assembly and function of ribosomes by interacting with rRNA and supporting the translation of mRNA into amino acids.

Biological function summary

RPS10 contributes to the formation and stability of the ribosomal 40S subunit. It works as part of the ribosome complex important for initiating and propelling protein synthesis. This ribosomal complex performs a central role in cellular growth and proliferation as it aids in assembling polypeptide chains that proteins need for diverse cellular processes. By doing so it supports a wide range of physiological functions.

Pathways

RPS10 contributes to protein synthesis through the translational initiation and elongation pathways. Its participation supports the accurate decoding and synthesis of proteins which are critical to cellular metabolism and growth. RPS10 interacts with other ribosomal proteins such as RPS12 and RPS14 to maintain these pathways' function and efficiency helping regulate cellular homeostasis and response to environmental stimuli.

RPS10 mutations can link to Diamond-Blackfan Anemia a condition marked by failed red blood cell production impacting patient health. Moreover aberrations in RPS10 link to various cancers such as breast cancer due to its role in protein synthesis and cell growth. RPS10 interacts with proteins like RPL5 another ribosomal protein within these disease contexts suggesting a broader contribution of ribosomal dysfunction in disease pathogenesis.

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製品の性状

製品の状態

Liquid

補足情報

ab140736 was purified using conventional chromatography techniques.

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一般的な情報

機能

Component of the 40S ribosomal subunit (PubMed : 23636399). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 23636399).

配列の類似性

Belongs to the eukaryotic ribosomal protein eS10 family.

翻訳後修飾

Methylated by PRMT5. Methylation is necessary for its interaction with NPS1, its localization in the granular component (GC) region of the nucleolus, for the proper assembly of ribosomes, protein synthesis and optimal cell proliferation.. Monoubiquitinated by ZNF598 when a ribosome has stalled during translation of poly(A) sequences, leading to preclude synthesis of a long poly-lysine tail and initiate the ribosome quality control (RQC) pathway to degrade the potentially detrimental aberrant nascent polypeptide (PubMed:28065601, PubMed:28132843, PubMed:28685749, PubMed:32011234, PubMed:36302773). Deubiquitinated by OTUD3 and USP21, antagonizing ZNF598 activity (PubMed:32011234). Deubiquitinated by OTUD1, antagonizing ZNF598 activity and stimulating formation of polysomes: deubiquitination by OTUD1 promotes stability and translation of a subset mRNAs with a high abundance of rare codons can limit the translation rate (PubMed:36445135). Deubiquitinated by USP10 (PubMed:31981475).

細胞内局在性

Nucleus

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製品プロトコール

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ターゲットの情報

Component of the 40S ribosomal subunit (PubMed : 23636399). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 23636399).

See full target information 40S ribosomal protein S10

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Abcam product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Recombinant Human RPS10 protein (His tag N-Terminus)