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AB205214

Recombinant Human RPLP0 protein

Recombinant Human RPLP0 protein

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Recombinant Human RPLP0 protein is a Human Full Length protein, in the 2 to 317 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

別名を表示する

Large ribosomal subunit protein uL10, 60S acidic ribosomal protein P0, 60S ribosomal protein L10E, RPLP0

Key facts

精製度

>90% SDS-PAGE

発現系

Escherichia coli

タグ

T7 tag N-Terminus His tag N-Terminus TEV tag N-Terminus

アプリケーション

SDS-PAGE

applications

生物活性

No

アクセッション番号

P05388

アニマルフリー

No

キャリアフリー

No

Human

バッファー組成

pH: 8 Constituents: 0.32% Tris HCl

storage-buffer

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

配列情報

[{"sequence":"MASMTGGQQMGRGHHHHHHENLYFQGGEFPREDRATWKSNYFLKIIQLLDDYPKCFIVGADNVGSKQMQQIRMSLRGKAVVLMGKNTMMRKAIRGHLENNPALEKLLPHIRGNVGFVFTKEDLTEIRDMLLANKVPAAARAGAIAPCEVTVPAQNTGLGPEKTSFFQALGITTKISRGTIEILSDVQLIKTGDKVGASEATLLNMLNISPFSFGLVIQQVFDNGSIYNPEVLDITEETLHSRFLEGVRNVASVCLQIGYPTVASVPHSIINGYKRVLALSVETDYTFPLAEKVKAFLADPSAFVAAAPVAAATTAAPAAAAAPAKVEAKEESEESDEDMGFGLFD","proteinLength":"Full Length","predictedMolecularWeight":"37.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":317,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P05388","tags":[{"tag":"T7","terminus":"N-Terminus"},{"tag":"His","terminus":"N-Terminus"},{"tag":"TEV","terminus":"N-Terminus"}]}]

出荷温度及び保存条件

出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-80°C
False

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

RPLP0 also known as 60S acidic ribosomal protein P0 is an integral component of the ribosomal machinery. It acts as a scaffold within the large 60S subunit of the ribosome which plays a fundamental role in protein synthesis. The protein has an approximate mass of 34.4 kDa. RPLP0 is ubiquitously expressed across various tissues indicating its essential role in cellular biology. Its expression is well-documented in highly proliferative tissues and organs reflecting the ribosome's central role in maintaining cellular growth and division.
Biological function summary

RPLP0 participates actively in ribosome structure and function. It forms part of the ribosomal stalk together with the P1 and P2 proteins facilitating interactions with GTPase translation factors during protein synthesis. This ribosomal complex is important for elongation phase of protein translation where it actively assists in the accurate and efficient elongation of polypeptide chains. By participating in the ribosome's structural framework RPLP0 drives the translation process influencing protein output in cells. This makes it essential for overall protein homeostasis.

Pathways

RPLP0 is deeply embedded within the processes of translation and ribosome biogenesis. It associates with the major pathway of ribosome assembly wherein new ribosomes are synthesized and configured. It functions alongside other ribosomal proteins like RPLP1 and RPLP2 which work together to maintain efficient protein synthesis. The interaction among these proteins within the translation pathway highlights the integrated nature of RPLP0 in facilitating cellular protein production.

RPLP0 has notable implications in cancer and Diamond-Blackfan anemia (DBA). Aberrations in ribosomal proteins including RPLP0 have been linked to oncogenesis where disrupted protein synthesis contributes to uncontrolled cellular proliferation. Additionally alterations in RPLP0 have associations with DBA a ribosomopathy characterized by defective red blood cell production. Within these disease contexts RPLP0's interaction with other ribosomal proteins like RPL11 can influence disease progression and cellular anomalies seen in such conditions.

製品の性状

製品の状態

Liquid

補足情報

ab205214 was expressed in E.coli as inclusion bodies, refolded using a unique “temperature shift inclusion body refolding” technology, chromatographically purified and sterile-filtered.

一般的な情報

機能

Ribosomal protein P0 is the functional equivalent of E.coli protein L10.

配列の類似性

Belongs to the universal ribosomal protein uL10 family.

翻訳後修飾

Ubiquitinated at Lys-264 by RNF14 and RNF25 in response to ribosome collisions (ribosome stalling).

細胞内局在性

Nucleus

製品プロトコール

ターゲットの情報

Ribosomal protein P0 is the functional equivalent of E.coli protein L10.
See full target information 60S acidic ribosomal protein P0

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