Recombinant Human RNF168 protein (ab153613)
Key features and details
- Expression system: Wheat germ
- Tags: GST tag N-Terminus
- Suitable for: ELISA, WB
製品の詳細
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製品名
Recombinant Human RNF168 protein -
発現系
Wheat germ -
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
MALPKDAIPSLSECQCGICMEILVEPVTLPCNHTLCKPCFQSTVEKASLC CPFCRRRVSSWTRYHTRRNSLVNVELWTIIQKHYPRECKLRASGQESEEV ADDYQPVRLLSKPGELRREYEEEISKVAAERRASEEEENKASEEYIQRLL AEEEEEEKRQAEKRRRAMEEQLKSDEELARKLSIDINNFCEGSISASPLN SRKSDPVTPKSEKKSKNKQRNTGDIQKYLTPKSQFGSASHSEAVQEVRKD SVSKDIDSSDRKSPTGQDTEIEDMPTLSPQISLGVGEQGADSSIESPMPW LCACGAEWYHEGNVKTRPSNHGKELCVLSHERPKTRVPYSKETAVMPCGR TESGCAPTSGVTQTNGNNTGETENEESCLLISKEISKRKNQESSFEAVKD PCFSAKRRKVSPESSPDQEETEINFTQKLIDLEHLLFERHKQEEQDRLLA LQLQKEVDKEQMVPNRQKGSPDEYHLRATSSPPDKVLNGQRKNPKDGNFK RQTHTKHPTPERGSRDKNRQVSLKMQLKQSVNRRKMPNSTRDHCKVSKSA HSLQPSISQKSVFQMFQRCTK -
領域
1 to 571 -
タグ
GST tag N-Terminus
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特性
Our Abpromise guarantee covers the use of ab153613 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
ELISA
Western blot
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00
Constituents: 0.31% Glutathione, 0.79% Tris HCl
関連情報
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別名
- E3 ubiquitin protein ligase RNF168
- E3 ubiquitin-protein ligase RNF168
- FLJ35794
see all -
機能
E3 ubiquitin-protein ligase required for accumulation of repair proteins to sites of DNA damage. Acts with UBE2N/UBC13 to amplify the RNF8-dependent histone ubiquitination. Recruited to sites of DNA damage at double-strand breaks (DSBs) by binding to ubiquitinated histone H2A and ubiquitinates histone H2A and H2AX, leading to amplify the RNF8-dependent H2A ubiquitination and promoting the formation of 'Lys-63'-linked ubiquitin conjugates. This leads to concentrate ubiquitinated histones H2A and H2AX at DNA lesions to the threshold required for recruitment of TP53BP1 and BRCA1. -
パスウェイ
Protein modification; protein ubiquitination. -
関連疾患
Defects in RNF168 are the cause of Riddle syndrome (RIDDLES) [MIM:611943]. Riddle syndrome is characterized by increased radiosensitivity, immunodeficiency, mild motor control and learning difficulties, facial dysmorphism, and short stature. Defects are probably due to impaired localization of TP53BP1 and BRCA1 at DNA lesions. -
配列類似性
Belongs to the RNF168 family.
Contains 1 RING-type zinc finger. -
ドメイン
The MIU motifs (motif interacting with ubiquitin) mediate the interaction with ubiquitin and the localization at sites of DNA damage. -
細胞内局在
Nucleus. Localizes to sites of DNA damage. - Information by UniProt
画像
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SDS-PAGE - Recombinant Human RNF168 protein (ab153613)ab153613 on a 12.5% SDS-PAGE stained with Coomassie Blue.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab153613 は論文での使用が確認できていません。
